MiR-29c Inhibits The Metastasis of Oral Squamous Cell Carcinoma And Promotes Its Cell Cycle Arrest By Targeting SERPINH1

Abstract

Background: A large number of studies have shown that the imbalance of miRNA and its target genes can promote the development of tumors. The purpose of this study was to investigate the biological role and molecular mechanism of SERPINH1 and its upstream regulator miR-29c in oral squamous cell carcinoma (OSCC).Material and methods: The expression levels of SERPINH1 and miR-29c were detected by RT-qPCR and Western blotting. The proliferation, apoptosis, metastasis and cell cycle were detected by MTT assay, would healing assay, transwell assay, flow cytometry and dual luciferase reporter assay.Results: SERPINH1 is highly expressed in patients with OSCC and can be used as a prognostic biomarker for OSCC. Cell function experiments showed that silencing the expression of SERPINH1 inhibited the proliferation and migration of OSCC cells and caused cell cycle arrest at S phase. Bioinformatics analysis showed that there is a binding site between miR-29c and SERPINH1, indicating that miR-29c may regulate the expression of SERPINH1. In addition, miR-29c overexpression inhibited the proliferation and metastasis of OSCC cells, and subsequent rescue experiment showed that SERPINH1 overexpression can reverse the inhibitory effect of miR-29c in OSCC cell proliferation, migration, apoptosis and cell cycle arrest.Conclusions: MiR-29c can regulate the proliferation, migration, invasion and cell cycle of OSCC cells by targeting SERPINH1.