miR-301a Deficiency Attenuates the Macrophage Migration and Phagocytosis through YY1/CXCR4 Pathway

Abstract

(1) Background: the miR-301a is well known involving the proliferation and migration of tumor cells. However, the role of miR-301a in the migration and phagocytosis of macrophages is still unclear. (2) Methods: sciatic nerve injury, liver injury models, as well as primary macrophage cultures were prepared from the miR-301a knockout (KO) and wild type (WT) mice to assess the macrophage’s migration and phagocytosis capabilities. Targetscan database analysis, Western blotting, siRNA transfection, and CXCR4 inhibition or activation were performed to reveal miR301a’s potential mechanism. (3) Results: the macrophage’s migration and phagocytosis were significantly attenuated by the miR-301a KO both in vivo and in vitro. MiR-301a can target Yin-Yang 1 (YY1), and miR-301a KO resulted in YY1 up-regulation and CXCR4 (YY1′s down-stream molecule) down-regulation. siYY1 increased the expression of CXCR4 and enhanced migration and phagocytosis in KO macrophages. Meanwhile, a CXCR4 inhibitor or agonist could attenuate or accelerate, respectively, the macrophage migration and phagocytosis. (4) Conclusions: current findings indicated that miR-301a plays important roles in a macrophage’s capabilities of migration and phagocytosis through the YY1/CXCR4 pathway. Hence, miR-301a might be a promising therapeutic candidate for inflammatory diseases by adjusting macrophage bio-functions.

miR-329b-5p Affects Sheep Intestinal Epithelial Cells against Escherichia coli F17 Infection

“…When damage occurs in the body, cells migrate quickly to repair the damage [76]. The results of Xu et al found that miR-301a knockout mice had reduced macrophage migration capacity, which affected subsequent damage repair in damaged tissues [77]. Liu et al discovered that miR-874-3p upregulation diminishes the migration capability of osteosarcoma cells [78].…”

Dissecting the roles and clinical potential of YY1 in the tumor microenvironment

“…Indicated that it may be a competitive relationship between YY1 and COX-2 promoter, p300 and HDAC1/2 after lipopolysaccharide treatment (88). In addition, Xu et al indicates that miR-301a/YY1/CXCR4 signaling pathway plays an important role in macrophage migration and phagocytosis (87). Hence, these results strongly suggest that YY1 is critically important for the tumor immune microenvironment.…”

“…In addition, Xu et al. indicates that miR-301a/YY1/CXCR4 signaling pathway plays an important role in macrophage migration and phagocytosis ( 87 ). Hence, these results strongly suggest that YY1 is critically important for the tumor immune microenvironment.…”

Regulation of immune cells by miR-451 and its potential as a biomarker in immune-related disorders: a mini review

“…Additionally, miRNAs exert a considerable influence on the migration and phagocytic functions of macrophages. As an illustration, the deletion of miR-301a diminishes macrophage migration and phagocytosis through the YY1/CXCR4 signaling pathway ( 61 ). Despite the abundance of research, the complex interactions between miRNAs and macrophages demand further comprehensive exploration.…”