miR‑30a regulates the proliferation and invasion of breast cancer cells by targeting Snail

Abstract

The present study aims to investigate the effect of miR-30a on the proliferative and invasive abilities of breast cancer cells, and to observe the role of miR-30a in the pathogenesis of breast cancer. With the increase of pathological grade and malignant degree of breast cancer cells, the miR-30a expression level gradually decreased (P<0.01). Transfection with miR-30a mimic significantly inhibited the proliferative and invasive ability of SK-BR-3 cells (P<0.01), while transfection with anti-miR-30a significantly improved the proliferative and invasive ability of these cells (P<0.01). It was revealed using bioinformatic methods that Snail was the functional target gene of miR-30a, and this was verified by the results of a luciferase reporter gene assay. The results of analysis of Snail expression in breast cancer tissues and breast cancer cells revealed that with the increase in pathological grade and malignant degree of breast cancer cells, Snail expression levels gradually increased (P<0.01). Western blotting revealed that miR-30a significantly inhibited Snail expression in SK-BR-3 cells, upregulated the expression of EMT-associated E-cadherin, and downregulated the expression of EMT-associated N-cadherin and Vimentin. MiR-30a was able to affect the proliferation and invasion of breast cancer cells by regulating Snail expression, and therefore has a regulatory effect on the occurrence and development of breast cancer.

Induction of Apoptosis and Regulation of MicroRNA Expression by (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) Treatment on MCF-7 Breast Cancer Cells

“…Real-time quantitative PCR was performed on SNAIL and VEGF, which were the target of miR-30a [ 48 ] and miR-3195 [ 31 ]. One microgram (μg) of total RNA was reverse transcripted using the QuantiTect Reverse Transcription Kit (Qiagen, USA) according to the manufacturer protocol.…”

Interplay among SNAIL Transcription Factor, MicroRNAs, Long Non-Coding RNAs, and Circular RNAs in the Regulation of Tumor Growth and Metastasis

“…One of the crucial regulators of SNAIL expression widely described in the literature is the miR-30 family. Members of this family target the 3 UTR of SNAIL mRNA in non-small cell lung carcinoma [62], breast cancer [63], pancreatic cancer stem cells [64], melanoma [65], esophageal squamous cell carcinoma [66], rhabdomyosarcoma [14], or in hepatocytes [67,68]. This inhibition usually regulates EMT in epithelial tumor types, but in mesenchymal tumors, such as rhabdomyosarcoma, it may be responsible for non-canonical SNAIL action [14]; it might also be important in different processes, such as atherosclerosis [69].…”

“…The results described above are summarized in Table 1. bladder cancer [82] melanoma [83] gastric cancer [84] miR-30 family non-small cell lung carcinoma [62] breast cancer [63] pancreatic cancer [64] melanoma [65] esophageal squamous cell carcinoma [66] rhabdomyosarcoma [14] hepatocytes [67,68] breast carcinoma [71] lung carcinoma [71] ovarian cancer [72] pancreatic cancer [74] miR-122 hepatocellular carcinoma [95] miR-125b breast cancer [85] miR-130b diabetic nephropathy [100] miR-133 fibroblasts [99] miR-137 ovarian cancer [72] miR-153…”

Potential miRNAs for miRNA-Based Therapeutics in Breast Cancer

“… Roles of miRNAs. Summary of all the articles published from 2018 to 2020 in PubMed Database [ 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , …”