MiR-30c-5p/ROCK2 axis regulates cell proliferation, apoptosis and EMT via the PI3K/AKT signaling pathway in HG-induced HK-2 cells

Cui, Lianshun; Yu, M; Cui, Xinglei

doi:10.1515/biol-2020-0089

Cited by 12 publications

(7 citation statements)

References 35 publications

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“…Another target of miR-30c is the regulatory subunit of phosphoinositide-3-kinase (PI3K), which phosphorylates phosphatidylinositol 4,5-bisphosphate to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) which, in turn, allows the recruitment of AKT1 to the membrane [ 36 ]. This enzyme plays a key role in glucose tolerance improvement by facilitating the effect of insulin.…”

Section: Discussionmentioning

confidence: 99%

Potential Relationship between the Changes in Circulating microRNAs and the Improvement in Glycaemic Control Induced by Grape Pomace Supplementation

Léniz

Martínez-Maqueda

Quintela

et al. 2021

Foods

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MicroRNAs (miRNAs) represent important tools in medicine and nutrition as new biomarkers, and can act as mediators of nutritional and pharmacological interventions. The aim of the present study was to analyse the effect of grape pomace supplementation on the expression of seven selected miRNAs and their potential relationship with the observed positive effect on glycaemic control, in order to shed light on the mechanism underlying the beneficial effect of this dietary intervention. For this purpose, plasma samples were obtained from 49 subjects with metabolic syndrome. After supplementation with grape pomace (6 weeks), these subjects were categorised as responders (n = 23) or non-responders (n = 26) according to the changes in their fasting insulin rate. MiRNA expression at baseline and at the end of the supplementation was analysed by RT-PCR, and the MiRecords Database was used to identify potential target genes for the studied miRNAs. The increase observed in miR-23a in the whole cohort was present in both subgroups of participants. The increase in miR-181a was significant among non-responders but not responders. The decrease in miR-30c and miR-222 was found in the responders, but not in the non-responders. No changes were observed in miR-10a, miR-151a, miR-181a, and miR-let-7a expressions. After analysing these results, a potential involvement of the reduced expression of miR-30c and miR-222, two microRNAs associated with insulin resistance and diabetes, in the improvement of glycaemic control produced by grape pomace administration, can be proposed. Further research is needed to confirm the involvement of glycolytic enzymes, PI3K, AMPK, and IRS-1 in the effect of grape pomace, as suggested by the changes induced in microRNAs.

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Section: Discussionmentioning

confidence: 99%

Potential Relationship between the Changes in Circulating microRNAs and the Improvement in Glycaemic Control Induced by Grape Pomace Supplementation

Léniz

Martínez-Maqueda

Quintela

et al. 2021

Foods

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“…For in vitro transfection, 100 nM miR-30c-5p mimic (sequence: 5'-UGUAAACAUCCUACACUCUCAGC-3'), miR-30c-5p inhibitor (sequence: 5'-GCUGAGAGUGUAGGAUGUUUACU-3'), miR-30c-5p mimics control (sequence: 5'-CUAACGCAUGCACAGUCGUACG-3') ( 35 ), miR-30c-5p inhibitor control (sequence: 5'-CAGUACUUUUGUGUAGUACAA-3') and, which were from Nanjing Synthgene Medical Technology Co., Ltd, were transfected into RPM cells using Lipofectamine 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) for 48 h at 37˚C according to the manufacturer's instructions. miR-30c mimic was co-transfected with 5 µg plasmid pcDNA3.1-SOX9-Flag (Nanjing Synthgene Medical Technology, Co., Ltd.).…”

Section: Methodsmentioning

confidence: 99%

miR‑30c reduces myocardial ischemia/reperfusion injury by targeting SOX9 and suppressing pyroptosis

Nie

Zhou

et al. 2023

Exp Ther Med

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MicroRNAs (miRNAs or miRs) are commonly involved in regulating myocardial ischemia/reperfusion (I/R) injury by binding and silencing their target genes. However, whether miRNAs regulate myocardial I/R-induced pyroptosis remains unclear. The present study established an in vivo rat model of myocardial I/R injury and in vitro hypoxia/reoxygenation (H/R) injury model in rat primary cardiomyocytes to investigate the function and the underlying mechanisms of miRNAs on I/R injury-induced pyroptosis. RNA sequencing was utilized to select the candidate miRNAs between normal and I/R group. Reverse transcription-quantitative PCR and western blotting were performed to detect candidate miRNAs (miR-30c-5p, also known as miR-30c) and SRY-related high mobility group-box gene 9 (SOX9) expression, as well as expression of pyroptosis-associated proteins (NF-κB, ASC, caspase-1, NLRP3) in the myocardial I/R model. ELISA was used to measure pyroptosis-associated inflammatory markers IL-18 and IL-1β. Moreover, the link between miR-30c and SOX9 was predicted using bioinformatics and luciferase reporter assay. In myocardial I/R injured rats, miR-30c was downregulated, while the expression of SOX9 was upregulated. Overexpression of miR-30c inhibited pyroptosis both in vivo and in vitro . Furthermore, miR-30c negatively regulated SOX9 expression by binding its 3'untranslated region. In conclusion, the miR-30c/SOX9 axis decreased myocardial I/R injury by suppressing pyroptosis, which may be a potential therapeutic target.

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“…Existing evidence suggests that miR-30c attenuates cardiovascular dysfunctions [51][52][53], hyperglycemia [54][55][56], obesity [57], atherosclerosis [58][59][60], dyslipidemia [17], and hepatic lipoprotein production [61,62], which constitute major risk factors in stroke and other neurological disorders. MiR-30c potentiates prosurvival anti-apoptotic pathways [63][64][65]. Experimental findings indicated that in the initial stages of ischemia suppression of autophagy by miR-30c may worsen neurodegeneration in the spinal cord I/R injury model [66].…”

Section: Role Of Mir-30c In Neurological Disordersmentioning

confidence: 99%

Emerging Role of MicroRNA-30c in Neurological Disorders

Kumar

Li²

2022

IJMS

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MicroRNAs (miRNAs or miRs) are a class of small non-coding RNAs that negatively regulate the expression of target genes by interacting with 3′ untranslated regions of target mRNAs to induce mRNA degradation and translational repression. The miR-30 family members are involved in the development of many tissues and organs and participate in the pathogenesis of human diseases. As a key member of the miR-30 family, miR-30c has been implicated in neurological disorders such as Alzheimer’s disease, Parkinson’s disease, multiple sclerosis, and stroke. Mechanistically, miR-30c may act as a multi-functional regulator of different pathogenic processes such as autophagy, apoptosis, endoplasmic reticulum stress, inflammation, oxidative stress, thrombosis, and neurovascular function, thereby contributing to different disease states. Here, we review and discuss the biogenesis, gene regulation, and the role and mechanisms of action of miR-30c in several neurological disorders and therapeutic potential in clinics.

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MiR-30c-5p/ROCK2 axis regulates cell proliferation, apoptosis and EMT via the PI3K/AKT signaling pathway in HG-induced HK-2 cells

Cited by 12 publications

References 35 publications

Potential Relationship between the Changes in Circulating microRNAs and the Improvement in Glycaemic Control Induced by Grape Pomace Supplementation

Potential Relationship between the Changes in Circulating microRNAs and the Improvement in Glycaemic Control Induced by Grape Pomace Supplementation

miR‑30c reduces myocardial ischemia/reperfusion injury by targeting SOX9 and suppressing pyroptosis

Emerging Role of MicroRNA-30c in Neurological Disorders

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