miR-31 promotes proliferation of colon cancer cells by targeting E2F2

Li, Tong; Luo, Wenjing; Liu, Kunmei; Lv, Xiaobo; Xi, Tao

doi:10.1007/s10529-014-1715-y

Cited by 52 publications

(44 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Thus, the spatial relationship between SAHF and γH2AX made us ask whether miR‐31a‐5p is able to regulate SAHF formation during this mechanism. According to a previous study (Li, Luo, Liu, Lv, & Xi, 2015) and bioinformatics analysis, we found E2F2, one of the E2Fs family, is the target gene of miR‐31a‐5p. Our results also confirmed that miR‐31a‐5p bound to the 3′UTR of E2F2 and resulted in the induction of SAHF.…”

Section: Discussionmentioning

confidence: 89%

MicroRNA‐31a‐5p from aging BMSCs links bone formation and resorption in the aged bone marrow microenvironment

et al. 2018

View full text Add to dashboard Cite

The alteration of age‐related molecules in the bone marrow microenvironment is one of the driving forces in osteoporosis. These molecules inhibit bone formation and promote bone resorption by regulating osteoblastic and osteoclastic activity, contributing to age‐related bone loss. Here, we observed that the level of microRNA‐31a‐5p (miR‐31a‐5p) was significantly increased in bone marrow stromal cells (BMSCs) from aged rats, and these BMSCs demonstrated increased adipogenesis and aging phenotypes as well as decreased osteogenesis and stemness. We used the gain‐of‐function and knockdown approach to delineate the roles of miR‐31a‐5p in osteogenic differentiation by assessing the decrease of special AT‐rich sequence‐binding protein 2 (SATB2) levels and the aging of BMSCs by regulating the decline of E2F2 and recruiting senescence‐associated heterochromatin foci (SAHF). Notably, expression of miR‐31a‐5p, which promotes osteoclastogenesis and bone resorption, was markedly higher in BMSCs‐derived exosomes from aged rats compared to those from young rats, and suppression of exosomal miR‐31a‐5p inhibited the differentiation and function of osteoclasts, as shown by elevated RhoA activity. Moreover, using antagomiR‐31a‐5p, we observed that, in the bone marrow microenvironment, inhibition of miR‐31a‐5p prevented bone loss and decreased the osteoclastic activity of aged rats. Collectively, our results reveal that miR‐31a‐5p acts as a key modulator in the age‐related bone marrow microenvironment by influencing osteoblastic and osteoclastic differentiation and that it may be a potential therapeutic target for age‐related osteoporosis.

show abstract

Section: Discussionmentioning

confidence: 89%

MicroRNA‐31a‐5p from aging BMSCs links bone formation and resorption in the aged bone marrow microenvironment

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Li T, et al . showed that E2F2 acted as a tumor suppressor in colon cancer by repressing the expression of survivin and regulating the expression of CCNA2, C-MYC, MCM4 and CDK2 [25]. Therefore, E2F2 may act as either a tumor suppressor or an activator in different cancer type.…”

Section: Discussionmentioning

confidence: 99%

Clinical performance of E2Fs 1-3 in kidney clear cell renal cancer, evidence from bioinformatics analysis

2017

View full text Add to dashboard Cite

Extensive research on the E2F transcription factor family has led to numerous insights that E2Fs were involved not only in proliferation and tumorigenesis but also in apoptosis and differentiation. In the present study, we analyzed the differential expression of E2Fs1-3 genes, and also evaluated the impact of E2Fs 1-3 genes expression on clinical outcome from the Cancer Genome Atlas (TCGA) database. The results showed that E2F1, E2F2 and E2F3 expression was increased in KIRC tissues than matched normal tissues (E2F1, P < 0.001; E2F2, P < 0.001, E2F3, P = 0.001), respectively. E2F1, E2F2 and E2F3 were significantly different in metastasis status, lymph node status, stage, and T stage in KIRC patients (all P < 0.01). E2F1 and E2F2 had the sensitivity of 96.1% and 93.1% and the specificity of 87.2% and 91.7% in discriminating KIRC from normal controls. High E2F1, E2F2 and E2F3 expression were correlated to worsen overall survival (all P < 0.01), and high E2F3 expression had worse disease free survival (P = 0.0404). Multivariate Cox regression analysis revealed that E2F1 and E2F3 were independent prognostic factors for overall survival. Taken together, E2F1 and E2F2 may serve as valuable diagnostic markers for KIRC. Moreover, E2F1, E2F2 and E2F3 could provide valuable prognostic information for KIRC patients.

show abstract

“…Increasing number of studies suggested that miRNAs affect the progression, pathogenesis classification, diagnosis, and prognosis of cancer 8, 9, 10, 11.Recently, increased miRNAs have been implicated in the digestive diseases 12, 13, 14.Several studies have found that miR‐21, miR‐17, miR‐31, miR‐15, and others were critically involved in the pathogenesis of colorectal cancer 15, 16, 17, 18. miR‐17‐92 cluster consisted of six miRNAs (miR‐17, miR‐18a, miR‐19a, miR‐20a, miR‐19b, miR‐92a) and two paralogs (miR‐106b and miR‐106a).…”

Section: Introductionmentioning

confidence: 99%

A miRNAs panel promotes the proliferation and invasion of colorectal cancer cells by targeting GABBR1

et al. 2016

View full text Add to dashboard Cite

MicroRNAs (miRNAs) have been implicated in the regulation of colorectal cancer. Despite the expression of miR‐17‐92 cluster in cancer has been gradually revealed, the role of each individual miRNAs in colorectal cancer still remains unclear. We studied the impact of miR‐106a/b, miR‐20a/b, and miR‐17 of miR‐17‐92 cluster on colorectal cancer cells. Real‐time quantitative polymerase chain reactions (RT‐PCR) were used to test these five miRNAs expression in colorectal cancer cell line HCT116. 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2‐H‐tetrazolium bromide (MTT) assays, Bromodeoxyuridine (BrdU), and Transwell invasion assays were used to explore the effects of these five miRNAs in colorectal cancer cells. Luciferase reporter assay, RT‐PCR, and western blotting were performed to validate the interaction of these five miRNAs with the gamma‐amino‐butyric acid type B receptor 1(GABBR1). We found that these five miRNAs were significantly upregulated in colorectal cancer samples compared with normal tissues. Forced expression of these five miRNAs significantly promoted HCT116 and HT‐29 cells proliferation and invasion. We further found that these five miRNAs function as oncogenes in colorectal cancer by specifically binding to the 3‐untranslated regions (3′UTR) of GABBR1.Furthermore, inhibition of GABBR1 could mimic the function of miRNAs in HCT116 cells, while overexpression of GABBR1 blocked the function of miRNAs‐promoted proliferation and invasion. In conclusion, miR‐106a/b, miR‐20a/b, and miR‐17 contribute to the proliferation and invasion of colorectal cancer by targeting their common target gene, GABBR1, and played a critical role in the proliferation and invasion of colorectal cancer.

show abstract

miR-31 promotes proliferation of colon cancer cells by targeting E2F2

Cited by 52 publications

References 17 publications

MicroRNA‐31a‐5p from aging BMSCs links bone formation and resorption in the aged bone marrow microenvironment

MicroRNA‐31a‐5p from aging BMSCs links bone formation and resorption in the aged bone marrow microenvironment

Clinical performance of E2Fs 1-3 in kidney clear cell renal cancer, evidence from bioinformatics analysis

A miRNAs panel promotes the proliferation and invasion of colorectal cancer cells by targeting GABBR1

Contact Info

Product

Resources

About