miR-3189-3p Mimics Enhance the Effects of S100A4 siRNA on the Inhibition of Proliferation and Migration of Gastric Cancer Cells by Targeting CFL2

Bian, Yue; Guo, Junfu; Qiao, Linlin; Sun, Xiuju

doi:10.3390/ijms19010236

Cited by 24 publications

(17 citation statements)

References 39 publications

(50 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RLIM knockdown significantly inhibits the proliferative and migratory capacities of prostate cancer cells 31 . High CFL2 expression is associated with poor overall survival in gastric cancer 32 . Therefore, miR-145-5p and miR-29b-3p play an important role in tumorigenesis and development of OSCC.…”

Section: Discussionmentioning

confidence: 98%

Upregulation of circ_0000199 in circulating exosomes is associated with survival outcome in OSCC

Luo

Liu

Guo

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Studies have found that circRnA in exosomes is associated with oral squamous cell carcinoma (oScc) progression. In this study, we examined the expression of circ_0000199 in circulating exosomes from patients with oScc and its role in the evaluation of relapse and prognosis. Real-time quantitative reverse transcription-polymerase chain reaction was performed to assess circ_0000199 expression in circulating exosomes from 108 patients with OSCC and 50 healthy people. Gain-and loss-functional experiments were performed to assess the role of circ_0000199 on cell proliferation and apoptosis in OSCC cells. Our results showed that the high level of circ_0000199 in circulating exosomes was significantly associated with betel quid chewing, tumor size, lymphatic metastasis, and TNM stage in patients with OSCC. In addition, the patients with high exosomal circ_0000199 had higher tumor recurrence rate and higher mortality rate than the patients with low exosomal circ_0000199. Overexpression of circ_0000199 promoted, while knockdown of circ_0000199 inhibited OSCC cell growth. Bioinformatics analysis predicted that circ_0000199 interacted with miR-145-5p and miR-29b-3p simultaneously, which were involved in multiple tumor-related signaling pathways. in conclusion, upregulation of circ_0000199 in circulating exosomes from patients with OSCC is positively associated with poor survival outcome. Circulating exosomal circ_0000199 can be used as a biomarker and potential therapeutic target for oScc. Oral squamous cell carcinoma (OSCC) is one of the most common malignant tumors in the world, accounting for 3% of malignant tumors 1. Although the treatment of OSCC has made breakthroughs in the past decades, the 5-year mortality rate is still less than 50% 2. Early diagnosis and early treatment are the keys to improve the survival rate of patients with OSCC. Exosomes have phospholipid bilayer membrane with the markers TSG101 and CD63. The size of exosomes ranges from 30 to 100 nm. These vesicles exist in almost all body fluids of the human body, including blood, serum, saliva, cerebrospinal fluid, and urine 3. Exosomes contain various substances, such as proteins, DNA, microRNA (miRNA), circular RNA (circRNA). These molecules can be transported to regulate the signaling pathways of the recipient cells 4. CircRNAs are covalently formed from the ends of a single RNA molecule, without 5′ and 3′ ribonucleotide end, and circRNAs are expressed abundantly, conserved and stably in cells with temporal and spatial specificity 5. CircRNAs can bind to miRNAs to inhibit their functions. Therefore, circRNA is also called miRNA sponge. With the continuous understanding of circRNA, researchers have found that circRNAs are widely involved in the occurrence and development of physiology and disease as well as tumors 6. As a new type of regulatory endogenous RNA, circRNAs are expected to become a tumor diagnostic marker and therapeutic target due to its characteristics such as stability, conservation, and tissue-specific expression 7. For example, circRNA_...

show abstract

Section: Discussionmentioning

confidence: 98%

Upregulation of circ_0000199 in circulating exosomes is associated with survival outcome in OSCC

Luo

Liu

Guo

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Furthermore, previous studies indicated that miR-149 is downregulated in breast cancer and colonic carcinoma cells and functions as a tumor suppressor by controlling breast epithelial cell migration and invasion, which is in agreement with the present results ( 26 , 27 ). miR-3189 has been previously reported to exert antitumor effects in glioblastoma and gastric cancer ( 28 , 29 ), miR-3677 may be used as a potential molecular marker to predict the prognosis of hepatic carcinoma patients ( 30 , 31 ), and miR-3917 may be useful for the early screening of high-risk populations and early diagnosis of lung cancer ( 32 ). Furthermore, miR-4999 and miR-6854, which have not been previously reported in tumors, to the best of our knowledge, may improve the accuracy of colon carcinoma prognostication.…”

Section: Discussionmentioning

confidence: 99%

MicroRNAs as biomarkers for the progression and prognosis of colon carcinoma

Hui

Wang

et al. 2018

Int J Mol Med

View full text Add to dashboard Cite

Early detection is critical for the treatment of colon carcinoma. However, current biomarkers for its diagnosis and prognosis are insufficient and improvement is required. Aberrantly expressed microRNAs (miRNAs/miRs) in colon carcinoma have been identified to function as potential diagnostic and prognostic biomarkers. In the present study, 245 differentially expressed miRNAs between colon carcinoma and normal tissues were identified by a bioinformatics analysis of a dataset from The Cancer Genome Atlas. A six-miRNA (miR-149, miR-3189, miR-3677, miR-3917, miR-4999 and miR-6854) prognostic prediction system was established, which is able to independently and effectively predict the prognosis of colon carcinoma patients [P<0.001, area under the receiver operating characteristic curve (AUC)=0.763]. Furthermore, the six miRNAs were highly correlated with the tumor-nodes-metastasis (TNM) stage and were able to distinguish between different stages (high vs. low TNM stage, P<0.001). Of note, combination of the six-miRNA signature and TNM stage provides an improved prediction of the patient's prognosis (AUC=0.797). Functional enrichment analysis revealed the possible mechanistic involvement of these predictive miRNAs in cancer-associated biological processes and pathways. Taken together, the present study demonstrated the promising potential of the novel six-miRNA model as an independent factor for the prediction of the progression and prognosis of colon carcinoma.

show abstract

“…QPCR of dcKO uteri showed Indian hedgehog signaling molecule (Ihh) 20-fold upregulated and patched 1 (Ptch1) and GLI family zinc finger 1 and 2 (Gli1 and Gli2) were 5-fold upregulated ( Figure 5C). High CFL2 expression was associated with poor survival in gastric cancer (40). Both high CFL2 and EPHA2 have been associated with increased proliferation in cancers (34,35,40).…”

Section: Unique Transcriptomic Profile With Loss Of Two Alleles Of DImentioning

confidence: 93%

“…High CFL2 expression was associated with poor survival in gastric cancer (40). Both high CFL2 and EPHA2 have been associated with increased proliferation in cancers (34,35,40).…”

Section: Unique Transcriptomic Profile With Loss Of Two Alleles Of DImentioning

confidence: 93%

PtenandDicer1loss causes poorly-differentiated endometrial adenocarcinoma in mice

Wang

Wendel

Emerson

et al. 2020

Preprint

View full text Add to dashboard Cite

Endometrial cancer remains the most common gynecological malignancy in the UnitedStates. While the loss of the tumor suppressor, PTEN (phosphatase and tensin homolog), is well studied in endometrial cancer, recent studies suggest that DICER1, the endoribonuclease responsible for miRNA genesis, also plays a significant role in endometrial adenocarcinoma. In an endometrial adenocarcinoma mouse model, which has a conditional uterine deletion of Pten, Dicer1 was also conditionally deleted.Conditional uterine deletion of Dicer1 and Pten resulted in high-penetrance, poorlydifferentiated endometrial adenocarcinomas. Poorly-differentiated endometrial adenocarcinomas expressed known markers of clear-cell adenocarcinoma, including Napsin A and HNF1B (hepatocyte nuclear factor 1 homeobox B). Adenocarcinomas were hormone-independent, and treatment with long-term progesterone did not mitigate poorly-differentiated adenocarcinoma, nor did it affect adnexal metastasis.Transcriptomic analyses of uteri or Ishikawa cells with deletion of DICER1 revealed unique transcriptomic profiles and global downregulation of miRNAs. Integration of downregulated miRNAs with upregulated mRNA targets revealed deregulated let-7 and miR-16 target genes, similar to published human DICER1-mutant endometrial cancers from TCGA (The Cancer Genome Atlas). Importantly, these miRNA-target genes, involved in ephrin-receptor and transforming growth factor-beta signaling, represent potential clinical targets for rare, yet deadly, poorly-differentiated endometrial adenocarcinomas in women. This mouse model represents poorly-differentiated endometrial adenocarcinoma and will allow for the discovery of novel mechanisms of hormone-independent endometrial adenocarcinoma from atrophic endometrium. Significance StatementEndometrial cancer is one of the few cancers with an increasing death rate in the United States. The most significant risk factor associated with death is high tumor grade, which occurs most frequently in postmenopausal women, where it develops within an atrophic endometrium. Here, we present a mouse model with conditional deletion of Dicer1, a key enzyme in miRNA genesis, and Pten, a tumor suppressor, that develops poorlydifferentiated, steroid hormone-independent, endometrial adenocarcinoma with adnexal metastasis. These high-grade adenocarcinomas develop from an atrophic endometrium and share molecular features with DICER1-mutant human endometrial adenocarcinomas. We anticipate that this preclinical model represents a move toward the discovery of novel mechanisms of hormone-independent development of endometrial adenocarcinoma from atrophic endometrium.

show abstract

miR-3189-3p Mimics Enhance the Effects of S100A4 siRNA on the Inhibition of Proliferation and Migration of Gastric Cancer Cells by Targeting CFL2

Cited by 24 publications

References 39 publications

Upregulation of circ_0000199 in circulating exosomes is associated with survival outcome in OSCC

Upregulation of circ_0000199 in circulating exosomes is associated with survival outcome in OSCC

MicroRNAs as biomarkers for the progression and prognosis of colon carcinoma

PtenandDicer1loss causes poorly-differentiated endometrial adenocarcinoma in mice

Contact Info

Product

Resources

About