MiR-3194-3p Inhibits Breast Cancer Progression by Targeting Aquaporin1

Abstract

Increasing evidence indicates that the Aquaporin1 (AQP1) aberrant expression may be related to a wide variety of human cancers, including breast cancer (BC). In the present study, we explore the effects and possible mechanism of miR-3194-3p on the biological behaviors of BC. At first, miR-3194-3p is found to modulate AQP1 expression targeting the 3-UTR using miRNA target prediction algorithms. MiR-3194-3p expression is markedly downregulated, and AQP1 expression is upregulated in BC tissues compared with adjacent normal breast tissues. Moreover, the differential expression of miR-3194-3p and AQP1 are observed in four BC cells with different malignancy degree. Meanwhile, a significant negative correlation between AQP1 and miR-3194-3p expressions in tumor tissues from 30 BC patients is revealed. miR-3194-3p mimic remarkably inhibits cell proliferation, migration, and invasion as well as promotes apoptosis in MDA-MB-231 cells while miR-3194-3p inhibitors exert an opposite role in MCF-7 cells. Dual-luciferase reporter system demonstrates that AQP1 is a direct target gene of miR-3194-3p. Overexpression of AQP1 by pBABE-puro-AQP1 vector partially abrogates the effect of miR-3194-3p mimic in MDA-MB-231 cells. In short, our results suggest that miR-3194-3p suppresses BC cell proliferation, migration, and invasion by targeting AQP1, providing a novel insight into BC tumorigenesis and treatment.

S-Adenosylmethionine Inhibits Cell Growth and Migration of Triple Negative Breast Cancer Cells through Upregulating MiRNA-34c and MiRNA-449a

“…Emerging evidence suggests that miRNAs play important roles in the pathogenesis of many types of human cancers by modulating different genes in the context of signaling pathways involved in tumor promotion or suppression [ 32 , 33 , 34 , 35 , 36 , 37 , 38 ].…”

“…Several studies have demonstrated the important role of miRNAs in the regulation of all essential cellular bioprocesses, including proliferation, differentiation, apoptosis, stress response, and migration [ 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 ]. MiRNA research has become increasingly attractive as evidence is emerging that miRNAs act as key regulators in the pathogenesis of diseases, including cancer.…”

“…Among non-coding RNAs, microRNAs (miRNA or miR) have emerged, in the last decade, as key players in tumorigenesis, through the post-transcriptional regulation of the main modulators involved in cell cycle progression, apoptosis, autophagy, as well as migration and invasion [ 32 , 33 , 34 , 35 ]. Many studies have demonstrated that the aberrant expression of miRNAs plays a causal role in breast cancer progression and directly contributes to cell proliferation and metastasis [ 33 , 35 , 36 , 37 , 38 ]. Due to their fundamental role in tumorigenic and metastatic processes miRNAs have been indicated as key targets in the diagnosis, prognosis and therapy of cancer [ 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 ].…”

Inhibition of aquaporins as a potential adjunct to breast cancer cryotherapy

“…In contrast, MCF-7 cells express ER, PR and low levels of HER2 and are used as an in vitro model of hormone-responsive breast cancer types (48). The utilization of these 2 cell lines in experiments allows for the comparison of both breast cancer types (49)(50)(51)(52).…”

Long noncoding RNA KCNMB2-AS1 promotes the development of esophageal cancer by modulating the miR-3194-3p/PYGL axis

“…For example, miR-3194-3p is significantly downregulated in hepatocellular carcinoma (HCC) tissues and cells, and miR-3194-3p inhibits the migration, invasion and epithelial-mesenchymal transition (EMT) of HCC cells via suppressing Wnt/β-catenin signaling through targeting BCL9 transcription coactivator (BCL9) [ 25 ]. MiR-3194-3p suppresses cell proliferation, migration, and invasion as well as promotes cell apoptosis in bladder cancer by targeting Aquaporin 1 (AQP1) [ 26 ]. However, the precise mechanisms of miR-3194-3p involvement in tumorigenesis of ERSC remain unclear.…”