MiR-322/424 and -503 Are Induced during Muscle Differentiation and Promote Cell Cycle Quiescence and Differentiation by Down-Regulation of Cdc25A

Sarkar, Sukumar; Dey, Bijan K.; Dutta, Anindya

doi:10.1091/mbc.e10-01-0062

Cited by 189 publications

(166 citation statements)

References 50 publications

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“…4E). In line with these results, the expression levels of cyclin D1 (Ccnd1), cyclin E (Ccne1), and Cdc25a cell cycle genes (45,46) resulted in downregulation (Fig. 4F).…”

Section: Figsupporting

confidence: 77%

Novel Long Noncoding RNAs (lncRNAs) in Myogenesis: a miR-31 Overlapping lncRNA Transcript Controls Myoblast Differentiation

Ballarino

Cazzella

D’Andrea

et al. 2015

Molecular and Cellular Biology

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Transcriptome analysis allowed the identification of new long noncoding RNAs differentially expressed during murine myoblast differentiation. These transcripts were classified on the basis of their expression under proliferating versus differentiated conditions, muscle-restricted activation, and subcellular localization. Several species displayed preferential expression in dystrophic (mdx) versus wild-type muscles, indicating their possible link with regenerative processes. One of the identified transcripts, lnc-31, even if originating from the same nuclear precursor of miR-31, is produced by a pathway mutually exclusive. We show that lnc-31 and its human homologue hsa-lnc-31 are expressed in proliferating myoblasts, where they counteract differentiation. In line with this, both species are more abundant in mdx muscles and in human Duchenne muscular dystrophy (DMD) myoblasts, than in their normal counterparts. Altogether, these data suggest a crucial role for lnc-31 in controlling the differentiation commitment of precursor myoblasts and indicate that its function is maintained in evolution despite the poor sequence conservation with the human counterpart.

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“…4E). In line with these results, the expression levels of cyclin D1 (Ccnd1), cyclin E (Ccne1), and Cdc25a cell cycle genes (45,46) resulted in downregulation (Fig. 4F).…”

Section: Figsupporting

confidence: 77%

Novel Long Noncoding RNAs (lncRNAs) in Myogenesis: a miR-31 Overlapping lncRNA Transcript Controls Myoblast Differentiation

Ballarino

Cazzella

D’Andrea

et al. 2015

Molecular and Cellular Biology

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“…We also note that Cdc25 and Ccnd messenger RNAs that are targets of miR-195/497 likely also play a role in cell cycle withdrawal before terminal myogenic differentiation. The downregulation of Cdc25 in differentiating C2C12 myoblasts in the presence of miR-322/424 and miR-503 promotes cell cycle arrest necessary for terminal differentiation, while the downregulation of Ccnd2 in neonatal myoblasts enhances muscle cell fusion 32,33 . It remains to be seen whether miR-195/497 are also involved in cell cycle arrest during the terminal differentiation of myoblasts, and whether MuSCs require similar miRNA regulation of Cdc25/Ccnd mRNAs to form skeletal muscle fibres.…”

Section: Resultsmentioning

confidence: 99%

miR-195/497 induce postnatal quiescence of skeletal muscle stem cells

2014

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Skeletal muscle stem cells (MuSCs), the major source for skeletal muscle regeneration in vertebrates, are in a state of cell cycle arrest in adult skeletal muscles. Prior evidence suggests that embryonic muscle progenitors proliferate and differentiate to form myofibres and also self-renew, implying that MuSCs, derived from these cells, acquire quiescence later during development. Depletion of Dicer in adult MuSCs promoted their exit from quiescence, suggesting microRNAs are involved in the maintenance of quiescence. Here we identified miR-195 and miR-497 that induce cell cycle arrest by targeting cell cycle genes, Cdc25 and Ccnd. Reduced expression of MyoD in juvenile MuSCs, as a result of overexpressed miR-195/ 497 or attenuated Cdc25/Ccnd, revealed an intimate link between quiescence and suppression of myogenesis in MuSCs. Transplantation of cultured MuSCs treated with miR-195/497 contributed more efficiently to regenerating muscles of dystrophin-deficient mice, indicating the potential utility of miR-195/497 for stem cell therapies.

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“…They are thought to play negative regulatory roles posttranscriptionally, causing mRNA cleavage or translation repression, by targeting the imperfect complementary sequences, usually located in the 3 0 -untranslated regions (UTRs) of mRNAs [1]. It has been reported that miRNAs are involved in various biological processes, including the cell cycle, differentiation and tumorigenesis [2][3][4]. Additionally, the temporal and spatial specificity that is frequently associated with development and differentiation is another characteristic of miRNA expression [5,6].…”

Section: Introductionmentioning

confidence: 99%

Cyclin T2: A novel miR‐15a target gene involved in early spermatogenesis

Teng

Wang

et al. 2011

FEBS Letters

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Edited by Tamas DalmayKeywords: miR-15a Cyclin T2 Cell differentiation Spermatogenesis MicroRNA microarray a b s t r a c t MicroRNAs (miRNAs) are posttranscriptional modulators of gene expression that play important roles in various biological processes. Spermatogenesis is a highly regulated process in which diploid spermatogonia eventually differentiate into haploid spermatozoa. In this study, we identified four differentially expressed miRNAs between two premeiotic male germ cells, made predictions about their putative targets, and confirmed cyclin T2 (Ccnt2) as a direct target of miR-15a. We also report that miR-15a inhibited muscle differentiation at least in part by targeting Ccnt2, which represents a novel interaction. Subsequently, miR-15a and Ccnt2 were profiled in developing mice testes to observe their inverse correlations in the postnatal 3-week period to understand their roles in spermatogenesis.

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MiR-322/424 and -503 Are Induced during Muscle Differentiation and Promote Cell Cycle Quiescence and Differentiation by Down-Regulation of Cdc25A

Abstract: This article describes a novel role of Cdc25A down-regulation during differentiation of proliferating myoblasts.

Cited by 189 publications

References 50 publications

Novel Long Noncoding RNAs (lncRNAs) in Myogenesis: a miR-31 Overlapping lncRNA Transcript Controls Myoblast Differentiation

Novel Long Noncoding RNAs (lncRNAs) in Myogenesis: a miR-31 Overlapping lncRNA Transcript Controls Myoblast Differentiation

miR-195/497 induce postnatal quiescence of skeletal muscle stem cells

Cyclin T2: A novel miR‐15a target gene involved in early spermatogenesis

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