MiR-325-3p Alleviates Acute Pancreatitis via Targeting RIPK3

Jia, Ao; Yang, Zhenwei; Shi, Ji-Yu; Liu, Jiaming; Zhang, Kun; Y, Cui

doi:10.1007/s10620-021-07322-6

Cited by 6 publications

(5 citation statements)

References 40 publications

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“…MiRNAs participate in the regulation of AP via posttranscriptional repressor target genes. For example, miR‐325‐3p reduces inflammation, edema, bleeding, and necrosis in AP by targeting RIPK3 28 . MiR‐20b‐5p regulates autophagy by directly targeting AKT3, inhibiting inflammation and apoptosis, and promoting angiogenesis, thereby attenuating severe AP 29 .…”

Section: Discussionmentioning

confidence: 99%

“…For example, miR‐325‐3p reduces inflammation, edema, bleeding, and necrosis in AP by targeting RIPK3. 28 MiR‐20b‐5p regulates autophagy by directly targeting AKT3, inhibiting inflammation and apoptosis, and promoting angiogenesis, thereby attenuating severe AP. 29 MiR‐339‐3p overexpression could regulate TRAF3 expression through the p38 pathway and inhibit cerulein‐induced AP cell inflammation and apoptosis.…”

Section: Discussionmentioning

confidence: 99%

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Phospholipase D2 targeted by miR‐5132‐5p alleviates cerulein‐induced acute pancreatitis via the Nrf2/NFκB pathway

Chen

Zhou

2023

Immunity Inflam & Disease

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Background: Acute pancreatitis (AP) is an inflammatory process unexpectedly occurring in the pancreas, imposing a substantial burden on healthcare systems. Herein, we aimed to clarify the mechanism of action of phospholipase D2 (PLD2) in cerulein-treated AR42J cells, affording valuable insights into the treatment of AP. Methods: The levels of PLD2, miR-5132-5p, inflammatory factors (interleukin [IL]−10, IL-6, and tumor necrosis factor-α), caspase-3 activity, and apoptosisrelated proteins (Bax and Bcl-2) in cerulein-treated AR42J cells were detected using reverse transcription-quantitative polymerase chain, caspase-3 activity, and Western blot analysis. Protein levels of nuclear Factor erythroid 2-Related Factor 2 (Nrf2) and nuclear factor-k-gene binding (NF-κB) were detected by Western blot analysis. TargetScan predicted upstream microRNAs (miRNAs) of PLD2, and the interaction between miR-5132-5p and PLD2 was verified using a luciferase assay.Results: In cerulein-treated AR42J cells, PLD2 levels were downregulated, while miR-5132-5p expression was upregulated. Overexpression of PLD2 attenuated the cerulein-mediated facilitatory effect on inflammation and apoptosis in AR42J cells by regulating the Nrf2/NFκB pathway. Luciferase reporter analysis revealed that miR-5132-5p targeted PLD2, and miR-5132-5p negatively regulated PLD2. Upregulation of miR-5132-5p expression exacerbated inflammation and apoptosis and reversed the protective effect of PLD2 overexpression on AP. Conclusion: PLD2 targeted by miR-5132-5p can attenuate cerulein-induced AP in AR42J cells via the Nrf2/NFκB pathway, providing therapeutic targets for patients with AP.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Phospholipase D2 targeted by miR‐5132‐5p alleviates cerulein‐induced acute pancreatitis via the Nrf2/NFκB pathway

Chen

Zhou

2023

Immunity Inflam & Disease

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“…A study using samples from AP patients and in vivo mouse models of AP showed that miR-325-3p levels were significantly reduced in the serum of AP patients and mice treated with cerulein [ 79 ]. Overexpression of miR-325-3p in pancreatic acinar cancer cells treated with cerulein reduced the activity of caspase-3 and attenuated apoptosis compared to controls.…”

Section: Acute Pancreatitismentioning

confidence: 99%

The Role of MicroRNAs in Pancreatitis Development and Progression

Patel

Almanzar

LaComb

et al. 2023

IJMS

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Pancreatitis (acute and chronic) is an inflammatory disease associated with significant morbidity, including a high rate of hospitalization and mortality. MicroRNAs (miRs) are essential post-transcriptional modulators of gene expression. They are crucial in many diseases’ development and progression. Recent studies have demonstrated aberrant miRs expression patterns in pancreatic tissues obtained from patients experiencing acute and chronic pancreatitis compared to tissues from unaffected individuals. Increasing evidence showed that miRs regulate multiple aspects of pancreatic acinar biology, such as autophagy, mitophagy, and migration, impact local and systemic inflammation and, thus, are involved in the disease development and progression. Notably, multiple miRs act on pancreatic acinar cells and regulate the transduction of signals between pancreatic acinar cells, pancreatic stellate cells, and immune cells, and provide a complex interaction network between these cells. Importantly, recent studies from various animal models and patients’ data combined with advanced detection techniques support their importance in diagnosing and treating pancreatitis. In this review, we plan to provide an up-to-date summary of the role of miRs in the development and progression of pancreatitis.

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“…Earlier studies have confirmed that its inhibition in wild-type mice alleviated the severity of AP and protected against tissue damage and necroptosis induced by TNF-α, which supports the fact that miR-21 is a miRNA that promotes necroptosis [ 99 ]. Jia et al [ 100 ] first verified that miR-325-3p was downregulated in AP patients and mouse models and decreased with an increase in the disease severity. It was then demonstrated that miR-325-3p inhibited the RIPK3/MLKL signaling pathway in acinar cells to slow down the development of AP.…”

Section: Pancreatitismentioning

confidence: 99%

Understanding Necroptosis in Pancreatic Diseases

Wang

Dong

et al. 2022

Biomolecules

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Intermediate between apoptosis and necrosis, necroptosis is a regulated caspase-independent programmed cell death that induces an inflammatory response and mediates cancer development. As our understanding improves, its role in the physiopathology of numerous diseases, including pancreatic diseases, has been reconsidered, and especially in pancreatitis and pancreatic cancer. However, the exact pathogenesis remains elusive, even though some studies have been conducted on these diseases. Its unique mechanisms of action in diseases are expected to bring prospects for the treatment of pancreatic diseases. Therefore, it is imperative to further explore its molecular mechanism in pancreatic diseases in order to identify novel therapeutic options. This article introduces recent related research on necroptosis and pancreatic diseases, explores necroptosis-related molecular pathways, and provides a theoretical foundation for new therapeutic targets for pancreatic diseases.

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MiR-325-3p Alleviates Acute Pancreatitis via Targeting RIPK3

Cited by 6 publications

References 40 publications

Phospholipase D2 targeted by miR‐5132‐5p alleviates cerulein‐induced acute pancreatitis via the Nrf2/NFκB pathway

Phospholipase D2 targeted by miR‐5132‐5p alleviates cerulein‐induced acute pancreatitis via the Nrf2/NFκB pathway

The Role of MicroRNAs in Pancreatitis Development and Progression

Understanding Necroptosis in Pancreatic Diseases

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