miR-335-5p suppresses gastric cancer progression by targeting MAPK10

Abstract

Background Recent studies have established the roles of microRNAs (miRNAs) in cancer progression. The aberrant expression of miR-335-5p has been reported in many cancers, including gastric cancer (GC). In this study, the precise roles of miR-335-5p in GC as well as the molecular mechanisms underlying its effects, including the role of its target MAPK10, were evaluated. Methods Quantitative real-time PCR was used to evaluate miR-335-5p levels in GC cell lines and tissues. MTT and colony formation assays were used to detect cell proliferation, and Transwell and wound-healing assays were used to evaluate the invasion and migration of GC cells. The correlation between levels of miR-335-5p and the cell cycle-related target gene mitogen-activated protein kinase 10 (MAPK10) in GC was analyzed. In addition, the candidate target was evaluated by a luciferase reporter assay, qRT-PCR, and western blotting. Results The levels of miR-335-5p were downregulated in GC tissues and cell lines. Furthermore, miR-335-5p inhibited the proliferation and migration of GC cells and induced apoptosis. Additionally, miR-335-5p arrested the cell cycle at the G1/S phase in GC cells in vitro. Levels of miR-335-5p and the cell cycle-related target gene MAPK10 in GC were correlated, and MAPK10 was directly targeted by miR-335-5p. Conclusions These data suggest that miR-335-5p is a tumor suppressor and acts via MAPK10 to inhibit GC progression.

Downregulation of circ‐ZNF644 alleviates LPS‐induced HK2 cell injury via miR‐335‐5p/HIPK1 axis

“…In this, we pointed out that circ‐ZNF644 could sponge miR‐335‐5p. MiR‐335‐5p has been discovered to be a tumor suppressor in many cancer 27,28 . MiR‐335‐5p was considered to be an inflammation‐related miRNA, and its upregulation could effectively inhibit inflammation 29 .…”

“…MiR-335-5p has been discovered to be a tumor suppressor in many cancer. 27,28 MiR-335-5p was considered to be an inflammation-related miRNA, and its upregulation could effectively inhibit inflammation. 29 Studies has shown that miR-335-5p was a regulator to be related to sepsis patient's mortality, which could suppress inflammatory responses, so it might be a candidate treatment target for patients with sepsis.…”

Establishment of a Novel Prognostic Prediction Model for Gastric Cancer Based on Necroptosis-Related Genes

“…There is increasing evidence that it acts as a microRNA target to play a tumor-promoting or tumorsuppressing role [23][24][25]. Gao et al [26] revealed that miR-335-5p suppressed GC progression by targeting MAPK10. In addition, we found that IFNA4 and IFNB1 were reported in cyclic GMP-AMP synthase-stimulator of interferon (cGAS-STING) related prognostic signatures in GC [27].…”

LncRNA DARS-AS1 aggravates the growth and metastasis of hepatocellular carcinoma via regulating the miR-3200-5p-Cytoskeleton associated protein 2 (CKAP2) axis

“…Similar to lncRNAs, microRNAs (miRNAs) are about 18–25 bases long, without a protein-coding function. In recent years, emerging evidence has pointed out that miRNAs regulate tumors’ malignant phenotype and then affect tumor progression [ 29 , 30 , 31 ]. In addition [ 32 ], DARS-AS1 can act as a competitive endogenous RNA to sponge miRNAs, thereby choking the effects of targeted miRNAs.…”