MiR-337-3p Promotes Adipocyte Browning by Inhibiting TWIST1

Vonhögen, Indira G. C.; Azzouzi, Hamid el; Olieslagers, Servé; Vasilevich, Aliaksei; Boer, Jan de; Tinahones, Francisco J.; Martins, Paula; Windt, León J. De; Murri, Mora

doi:10.3390/cells9041056

Cited by 20 publications

(11 citation statements)

References 33 publications

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“…MicroRNAs (miRNAs, miRs) are small, highly conserved, non-coding single-stranded ribonucleic acids (RNAs), which enhance messenger RNA (mRNA) degradation and/or inhibit protein translation by binding to the 3 -untranslated regions (UTRs) of target mRNAs [31][32][33][34]. They play essential regulatory roles in a number of biological processes, both in health and in disease, and have been extensively investigated in cardiovascular medicine [35][36][37][38].…”

Section: Introductionmentioning

confidence: 99%

miR-98 Regulates TMPRSS2 Expression in Human Endothelial Cells: Key Implications for COVID-19

et al. 2020

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The two main co-factors needed by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to enter human cells are angiotensin-converting enzyme 2 (ACE2) and transmembrane protease serine 2 (TMPRSS2). Here, we focused on the study of microRNAs that specifically target TMPRSS2. Through a bioinformatic approach, we identified miR-98-5p as a suitable candidate. Since we and others have shown that endothelial cells play a pivotal role in the pathogenesis of the coronavirus disease 2019 (COVID-19), we mechanistically validated miR-98-5p as a regulator of TMPRSS2 transcription in two different human endothelial cell types, derived from the lung and from the umbilical vein. Taken together, our findings indicate that TMPRSS2 represents a valid target in COVID-19 treatment, which may be achieved by specific non-coding-RNA approaches.

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Section: Introductionmentioning

confidence: 99%

miR-98 Regulates TMPRSS2 Expression in Human Endothelial Cells: Key Implications for COVID-19

et al. 2020

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“…Let-7 is one of the well-studied miRNAs which inhibits pre-adipocytes clonal expansion and terminal differentiation via targeting HMGA2 (Sun et al, 2009). MiR-337-3p can inhibit TWIST1 and promote transcription of genes participating in brown fat metabolism (Vonhögen et al, 2020). In circ_0000819, two miR-337-3p binding sites were identified.…”

Section: Discussionmentioning

confidence: 99%

Assessment of CircRNA Expression Profiles and Potential Functions in Brown Adipogenesis

Zhang

Sheng

et al. 2021

Front. Genet.

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Brown adipose tissue (BAT) is specialized for energy expenditure, thus a better understanding of the regulators influencing BAT development could provide novel strategies to defense obesity. Many protein-coding genes, miRNAs, and lncRNAs have been investigated in BAT development, however, the expression patterns and functions of circRNA in brown adipogenesis have not been reported yet. This study determined the circRNA expression profiles across brown adipogenesis (proliferation, early differentiated, and fully differentiated stages) by RNA-seq. We identified 3,869 circRNAs and 36.9% of them were novel. We found the biogenesis of circRNA was significantly related to linear mRNA transcription, meanwhile, almost 70% of circRNAs were generated by alternative back-splicing. Next, we examined the cell-specific and differentiation stage-specific expression of circRNAs. Compared to white adipocytes, nearly 30% of them were specifically expressed in brown adipocytes. Further, time-series expression analysis showed circRNAs were dynamically expressed, and 117 differential expression circRNAs (DECs) in brown adipogenesis were identified, with 77 upregulated and 40 downregulated. Experimental validation showed the identified circRNAs could be successfully amplified and the expression levels detected by RNA-seq were reliable. For the potential functions of the circRNAs, GO analysis suggested that the decreased circRNAs were enriched in cell proliferation terms, while the increased circRNAs were enriched in development and thermogenic terms. Bioinformatics predictions showed that DECs contained numerous binding sites of functional miRNAs. More interestingly, most of the circRNAs contained multiple binding sites for the same miRNA, indicating that they may facilitate functions by acting as microRNA sponges. Collectively, we characterized the circRNA expression profiles during brown adipogenesis and provide numerous novel circRNAs candidates for future brown adipogenesis regulating studies.

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“…The expression level of miR-337-3p in the BAT was significantly higher than that in the WAT. Also, the overexpression of miR-337-3p in brown preadipocytes resulted in the increased expression of mitochondrial markers, thus suggesting that miR-337-3p can enhance adipocyte browning [77]. MiR-30a is enriched in the BAT and the forced expression of miR-30a increases mitochondrial volume and mitochondrial respiration in human white adipocytes, making them characteristic of brown adipocytes [98].…”

Section: Regulates Adipose Tissue Thermogenesis and Energy Metabolism...mentioning

confidence: 99%

Regulation of Adipose Thermogenesis and its Critical Role in Glucose and Lipid Metabolism

Wang¹,

Qiu²,

Gan³

et al. 2022

Int. J. Biol. Sci.

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The function of the adipose tissue is influenced by complex interactions between genetics, epigenetics, and the environment, and its dysfunction can cause a variety of metabolic diseases, such as obesity or type 2 diabetes (T2D). The beige/brown adipose tissue plays a crucial role in regulating glucose and lipid metabolism by increasing energy metabolism to generate heat. The adipose tissue thermogenic program is a complex network that involves many signaling pathways regulated by coding RNAs (cRNAs) that encode transcription factor, and non-coding RNAs (ncRNAs) including microRNAs (miRNAs) and long noncoding RNAs (lncRNAs). This article discusses factors that regulate adipose tissue thermogenesis, including cRNAs and ncRNAs, and the important role of thermogenic adipose tissue in obesity-related metabolic syndrome. Several studies have shown that some cRNAs and ncRNAs can modulate the thermogenic function of adipose tissue in different ways. This article reviews the roles of cRNAs and ncRNAs in regulating thermogenesis in the beige/brown adipose tissue and the important role of the beige/brown adipose tissue in maintaining the balance of glucose and lipid metabolism in the body.

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MiR-337-3p Promotes Adipocyte Browning by Inhibiting TWIST1

Cited by 20 publications

References 33 publications

miR-98 Regulates TMPRSS2 Expression in Human Endothelial Cells: Key Implications for COVID-19

miR-98 Regulates TMPRSS2 Expression in Human Endothelial Cells: Key Implications for COVID-19

Assessment of CircRNA Expression Profiles and Potential Functions in Brown Adipogenesis

Regulation of Adipose Thermogenesis and its Critical Role in Glucose and Lipid Metabolism

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