MiR‐342‐3p inhibition promotes cell proliferation and invasion by directly targeting ID4 in pre‐eclampsia

Abstract

Aim This study aimed to explore the miR‐342‐3p expression in pre‐eclampsia (PE) placentas and confirm whether miR‐342‐3p exerts effects on proliferation and migration of HTR‐8/SVneo trophoblastic cells. Methods The PE placentas (n = 8) were taken from gravidas complicated by PE and delivered after 34 weeks. The chorionic plates and the basal plates were separately taken from the placenta disc near the position of umbilical cord insertion. RT‐qPCR was used to measure the expression of miR‐342‐3p in the chorionic plates and the basal plates. Cell invasion assay and MMT assay were used to assess the effects of miR‐342‐3p on proliferation and migration of HTR‐8/SVneo trophoblastic cells. Luciferase reporter assay and Western blotting were used to analyze the target of miR‐342‐3p and investigate the detailed mechanisms. Results The expression of miR‐342‐3p was upregulated in both basal plates and chorionic plates in patients with PE compared with healthy pregnant individuals. MiR‐342‐3p inhibitor suppressed the cell viability and invasion, and induced apoptosis in trophoblast cells. Furthermore, inhibitor of DNA binding (ID)‐4 (ID4) was a direct target of miR‐342‐3p, and knockdown of ID4 abrogated the regulation effect of miR‐342‐3p on cell viability, apoptosis and invasion. Conclusion Inhibition of miR‐342‐3p expression may suppress the occurrence of PE by targeting ID4 in vitro.

Inhibitory effect of miR342 on the progression of triple-negative breast cancer cells in vitro and in the mice model

“…It was demonstrated that ID4, as the direct target of miR342, is one of the essential players regulating cell proliferation and invasion. 26 , 27 Moreover, it was reported that miR342 could affect BRCA1 expression indirectly via direct inhibition of ID4 in MDA-MB-231 triple-negative breast cancer cells. 28 In fact, ID4 is an oncogene whose inhibitory role on the regulation of BRCA1 tumor suppressor factor was detected before.…”

MicroRNA signatures in plasma and plasma exosome during window of implantation for implantation failure following in-vitro fertilization and embryo transfer

“…Part et.al reported that miR-150G > A polymorphism was associated with RPL [ 27 ]. Researchers found that miR-342-3p can regulate trophoblasts proliferation, migration, and invasion [ 28 , 29 ]. Besides, miR-125b regulates endometrial receptivity, mediate embryo-maternal interactions [ 30 , 31 ].…”

“…Hub genes are of functional importance. Among the ten hub miRNAs, miR-150-5p, miR-150-3p, miR-342-5p, miR-146-3p, miR-124-3p, miR-342-5p, and miR-874-3p were also differentially dysregulated in both plasma and plasma exosomes, these miRNAs are reported to be related with embryo implantation [ 11 , 22 – 26 , 28 – 31 ]. Some of the other hub miRNAs have also been reported to be associated with embryo implantation failure.…”

Roles of noncoding RNAs in preeclampsia

“… [ 100 ] miR-454 placenta downregulated ALK7/EPHB4 miR-454 promotes the proliferation and invasion of trophoblast cells by inhibiting EPHB4 /ALK7 [ 84 , 85 ] miR-200a plasma upregulated EG-VEGF/TTR miR-200a suppressed primary cilia formation and inhibited trophoblast invasion. [ 101 , 102 ] miR-548c-5p placenta/ serum exosome downregulated PTPRO miR-548c-5p could inhibit the proliferation and activation of LPS-stimulated macrophages and decrease levels of inflammatory cytokines [ 103 ] miR-342-3p placenta upregulated PDGFRA/ID4 miR-342-3p was proposed to inhibit the proliferation, migration, invasion and G1/S phase transition of HTR8/SVneo cells [ 104 , 105 ] miR-431 placenta upregulated ZEB1 miR-431 inhibited the migration and invasion of trophoblastic cells [ 106 ] miR-221-3p placenta downregulated THBS2 miR-221-3p overexpression inhibited apoptosis, increased cell population at S phase, and decreased cell population at G0/G1 phase of HTR-8/SVneo cells [ 107 ] miR-152 placenta upregulated VEGF the increased miR-152 expression can promote the apoptosis of trophoblast cells. [ 108 ] miR-133 serum upregulated Rho/ROCK MiR-133 may affect the apoptosis of trophoblasts in the placenta tissues [ 109 ] miR-384 placenta/plasma upregulated PTBP3 Cell proliferation and migration were inhibited by miR-384 overexpression …”

“…In placenta, Wu et al have found that ROC of lnc TCL6 could reach to 0.8625 [ 138 ]. The ROC values of lnc BC030099 in the whole blood cells were 0.713 [ 104 ] and that of serum lnc AF085938, and that of lnc G36948 and lnc AK002210 were 0.7673, 0.7956 and 0.7575 respectively [ 178 ].…”