MiR-34a-5p promotes autophagy and apoptosis of ovarian granulosa cells via the Hippo-YAP signaling pathway by targeting LEF1 in chicken

Han, Shunshuan; Zhao, Xiyu; Zhang, Yao; Amevor, Felix Kwame; Tan, Bo; Ma, Mengen; Kang, Houyang; Wang, Jianping; Zhu, Qing; Yin, Huadong; Cui, Can

doi:10.1016/j.psj.2022.102374

Cited by 14 publications

(3 citation statements)

References 41 publications

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“…Apoptosis of granulosa cells is closely associated with follicular atresia ( Wang et al, 2023 ), process controlled by reproductive hormones and cytokines ( Santos et al, 2022 ; Bevilaqua et al, 2023 ; Grynberg et al, 2023 ; Li et al, 2023 ). Numerous studies performed in chicken, rodents and pigs have shown that proapoptotic and antiapoptotic factors contribute to follicular atresia triggering the apoptosis of granulosa cells ( Guo et al, 2019 ; Han et al, 2023 ; Ludwig et al, 2023 ). In the current study, we aimed, with the use of sRNA sequencing, to examine the effect of natural polyphenol RSV on apoptosis of porcine granulosa cells.…”

Section: Discussionmentioning

confidence: 99%

Identification of microRNAs implicated in modulating resveratrol-induced apoptosis in porcine granulosa cells

Zhang

Wang

Xie

et al. 2023

Front. Cell Dev. Biol.

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MicroRNAs (miRNAs) are small, noncoding RNAs that play a crucial role in the complex and dynamic network that regulates the apoptosis of porcine ovarian granulosa cells (POGCs). Resveratrol (RSV) is a nonflavonoid polyphenol compound that is involved in follicular development and ovulation. In previous study, we established a model of RSV treatment of POGCs, confirming the regulatory effect of RSV in POGCs. To investigate the miRNA-level effects of RSV on POGCs to reveal differentially expressed miRNAs, a control group (n = 3, 0 μM RSV group), a low RSV group (n = 3, 50 μM RSV group), and a high RSV group (n = 3, 100 μM RSV group) were created for small RNA-seq. In total, 113 differentially expressed miRNAs (DE-miRNAs) were identified, and a RT-qPCR analysis showed a correlation with the sequencing data. Functional annotation analysis revealed that DE-miRNAs in the LOW vs. CON group may be involved in cell development, proliferation, and apoptosis. In the HIGH vs. CON group, RSV functions were associated with metabolic processes and responses to stimuli, while the pathways were related to PI3K24, Akt, Wnt, and apoptosis. In addition, we constructed miRNA-mRNA networks related to Apoptosis and Metabolism. Then, ssc-miR-34a and ssc-miR-143-5p were selected as key miRNAs. In conclusion, this study provided an improved understanding of effects of RSV on POGCs apoptosis through the miRNA modulations. The results suggest that RSV may promote POGCs apoptosis by stimulating the miRNA expressions and provided a better understanding of the role of miRNAs combined with RSV in ovarian granulosa cell development in pigs.

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Section: Discussionmentioning

confidence: 99%

Identification of microRNAs implicated in modulating resveratrol-induced apoptosis in porcine granulosa cells

Zhang

Wang

Xie

et al. 2023

Front. Cell Dev. Biol.

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“…Anti-inflammatory, anti-cardiovascular, anticancerous, antineurodegenerative NF-κB/STAT3/ATF6, FLCN-AMPK-PGC-1α, circ_0000190/ miR-130a-3p, Notch-1 (Gao et al, 2023;Imam et al, 2021) Quercetin Digestive issues such as stomach cramps, diarrhea, or nausea Antioxidant activity, anti-inflammatory effects, modulation of immune response, inhibition of cancer cell growth, cardiovascular protective effects Nrf2, Nrf2/Heme oxygenase-1 (HO-1), MAPK, SIRT1/PGC-1α, Toll-like receptor 4 (TLR4)/NF-κB/ NOD-like receptor 3 (NLRP3) (Zamanian et al, 2023;Feng et al, 2023;Maurya & Vinayak, 2016;Shen et al, 2023;Singh et al, 2012;Zhang, Li, Liu, & Wang, 2022) Ellagic acid There is limited research on the adverse effects of ellagic acid, suggesting that it is generally safe for consumption Antioxidant, antiinflammatory, anticarcinogenic, antidiabetic, cardio protection activities TGF-β/Smad, insulin/ IGF-1, AMPK, PI3K/ Akt/FOXO3a, STAT3 (Bai et al, 2022;Kowshik et al, 2014;Lin et al, 2021;Park et al, 2022;Ratnam et al, 2006;Zhao Zj et al, 2023) Ferulic acid Gastrointestinal discomfort or allergic reactions when consuming high doses…”

Section: Resveratrolmentioning

confidence: 99%

Nanophytomedicine: A promising practical approach in phytotherapy

Sardarabadi,

Darvishi,

Zohrab

et al. 2024

Phytotherapy Research

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The long and rich history of herbal therapeutic nutrients is fascinating. It is incredible to think about how ancient civilizations used plants and herbs to treat various ailments and diseases. One group of bioactive phytochemicals that has gained significant attention recently is dietary polyphenols. These compounds are commonly found in a variety of fruits, vegetables, spices, nuts, drinks, legumes, and grains. Despite their incredible therapeutic properties, one challenge with polyphenols is their poor water solubility, stability, and bioavailability. This means that they are not easily absorbed by the body when consumed in essential diets. Because of structural complexity, polyphenols with high molecular weight cannot be absorbed in the small intestine and after arriving in the colon, they are metabolized by gut microbiota. However, researchers are constantly working on finding solutions to enhance the bioavailability and absorption of these compounds. This study aims to address this issue by applying nanotechnology approaches to overcome the challenges of the therapeutic application of dietary polyphenols. This combination of nanotechnology and phytochemicals could cause a completely new field called nanophytomedicine or herbal nanomedicine.

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“…MicroRNAs (miRNAs) are small, non-protein-coding RNAs that exist in animals, plants, and viruses [9,10]. These molecules play a key role in the posttranscriptional regulation of gene expression and affect various biological processes.…”

Section: Introductionmentioning

confidence: 99%

MiR-106a-5p by Targeting MAP3K2 Promotes Repair of Oxidative Stress Damage to the Intestinal Barrier in Prelaying Ducks

Zhang,

Luo,

Tang

et al. 2024

Animals

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Under caged stress conditions, severe disruptions in duck intestinal barrier function, which adversely affect economic performance, have been observed. MiRNAs play a crucial role in cellular processes, but the mechanisms underlying their involvement in repairing oxidative stress-induced damage to duck intestinal barriers have not been elucidated. We performed miRNA-seq and protein tandem mass tagging (TMT) sequencing and identified differentially expressed miRNAs and proteins in oxidative stress-treated ducks. Dual-luciferase reporter vector experiments, RT‒qPCR, and Western blotting revealed the regulatory role of apla-miR-106a-5p/MAP3K2 in intestinal barrier damage repair. The results showed that oxidative stress led to shortened villi and deepened crypts, impairing intestinal immune function. Significant downregulation of apla-miR-106a-5p was revealed by miRNA-seq, and the inhibition of its expression not only enhanced cell viability but also improved intestinal barrier function. TMT protein sequencing revealed MAP3K2 upregulation in caged-stressed duck intestines, and software analysis confirmed MAP3K2 as the target gene of apla-miR-106a-5p. Dual-fluorescence reporter gene experiments demonstrated direct targeting of MAP3K2 by apla-miR-106a-5p. RT‒qPCR showed no effect on MAP3K2 expression, while Western blot analysis indicated that MAP3K2 protein expression was suppressed. In summary, apla-miR-106a-5p targets MAP3K2, regulating gene expression at the transcriptional level and facilitating effective repair of intestinal barrier damage. This discovery provides new insights into the molecular mechanisms of physiological damage in ducks under caged stress, offering valuable guidance for related research.

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MiR-34a-5p promotes autophagy and apoptosis of ovarian granulosa cells via the Hippo-YAP signaling pathway by targeting LEF1 in chicken

Cited by 14 publications

References 41 publications

Identification of microRNAs implicated in modulating resveratrol-induced apoptosis in porcine granulosa cells

Identification of microRNAs implicated in modulating resveratrol-induced apoptosis in porcine granulosa cells

Nanophytomedicine: A promising practical approach in phytotherapy

MiR-106a-5p by Targeting MAP3K2 Promotes Repair of Oxidative Stress Damage to the Intestinal Barrier in Prelaying Ducks

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