miR‑34c‑5p targets Notch1 and suppresses the metastasis and invasion of cervical cancer

Wei, Huali; Wang, Xiaolan; XiuMin, Niu; Jiao, Ruili; Li, Xiaojuan; Wang, Sumei

doi:10.3892/mmr.2020.11759

Cited by 17 publications

(16 citation statements)

References 35 publications

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“…Yang [30] et aldemonstrated that miR-362 could work as an anti-oncomiR that suppressed proliferation and promoted apoptosis in CC cells via BAP31 and TGFβ/Smad pathway. Wei [31] et al verified that miR-34c-5p targeted Notch 1 and inhibited the metastasis and invasion of CC. It was reported that miR-381 targeted GPR34 to regulate the growth, migration and invasion of human CC cells [32].…”

Section: Discussionmentioning

confidence: 99%

MiR-26a-5p regulates proliferation, apoptosis, migration and invasion via inhibiting hydroxysteroid dehydrogenase like-2 in cervical cancer cell

Xiao

Liu

et al. 2022

BMC Cancer

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Background Evidences have indicated that miR-26a-5p regulates the malignant properties of various tumor cells. However, the influences of miR-26a-5p on proliferation, apoptosis and invasion are still vague in the cervical cancer (CC) cells. Methods The miRNA microarray and real-time quantitative PCR (RT-qPCR) analysis were utilized to detect the expression of miR-26a-5p in the patients with CC. Kaplan–Meier plotter was performed to evaluate the overall survival (OS) of the patients with CC. The CCK-8, flow cytometry, transwell and wound healing analyses were respectively used to analyze proliferation, migration and invasion in the CC cells. RT-qPCR, western blot and IHC analysis were executed to measure the expression of hydroxysteroid dehydrogenase like-2 (HSDL2) in the patients with CC. Bioinformatics and luciferase reporter assay were carried out to verify the relationship of miR-26a-5p and HSDL2. Results The expression of miR-26a-5p was downregulated and low expression of miR-26a-5p indicated a poor OS in patients with CC. Overexpression of miR-26a-5p significantly inhibited proliferation, migration and invasion, accelerated apoptosis in the Hela and C33A cells. The expression of HSDL2 was upregulated, and negatively correlated with miR-26a-5p in the patients with CC. HSDL2 was directly targeted by miR-26a-5p and rescue experiments displayed that HSDL2 partially abolished proliferation, apoptosis, migration, and invasion induced by miR-26a-5p in CC cells. Conclusions MiR-26a-5p alleviated progression of CC by suppressing proliferation, migration and invasion, promoting apoptosis through downregulating HSDL2.

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Section: Discussionmentioning

confidence: 99%

MiR-26a-5p regulates proliferation, apoptosis, migration and invasion via inhibiting hydroxysteroid dehydrogenase like-2 in cervical cancer cell

Xiao

Liu

et al. 2022

BMC Cancer

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“…lncRNA KCNQ1OT1 sponges miR-34c-5p to enhances osteosarcoma progression by ALDOA-enhanced aerobic glycolysis [ 31 ]. miR-34c-5p represses Notch1 and inhibits invasion and metastasis of cervical cancer [ 21 ]. miR-34c-5p regulates paclitaxel-induced apoptosis of lung cancer cells [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

“…It has been widely accepted that miRNAs play pivotal roles in the regulation of cancer development, as well as drug resistance, by directly targeting mRNAs [ 18 – 20 ]. miR-34c-5p is able to inhibit Notch1 and represses invasion and metastasis in cervical cancer [ 21 ]. SIRT6 targeted by miR-34c-5p contributes to proliferation of colon cancer by JAK2/STAT3 signaling [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Long Noncoding RNA MMP2-AS1 Contributes to Progression of Renal Cell Carcinoma by Modulating miR-34c-5p/MMP2 Axis

Fan

Niu

Ren

et al. 2022

Journal of Oncology

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Renal cell carcinoma (RCC) serves as a prevalent malignancy of urinary system and presents severe mortality and increasing incidence. Long noncoding RNAs (lncRNAs) have demonstrated critical roles in RCC development. Here, we were interested in the function of MMP2-AS1 during RCC progression. We observed that MP2-AS1 localized in both nucleus and cytoplasm of RCC cells using fluorescent in situ hybridization (FISH). The cell viability, proliferation, invasion, and migration of RCC cells were reduced by the depletion of MMP2-AS1. The MMP2-AS1 depletion-inhibited viability, proliferation, migration, and invasion of RCC cells were rescued by the overexpression of MMP2 in vitro. Consistently, the tumor growth of RCC cells was repressed by the depletion of MMP2-AS1 in the nude mice, while the overexpression of MMP2 could reverse this effect in vivo. Mechanically, we predicted the potential interaction of miR-34c-5p with both MMP2-AS1 and MMP2. The treatment of miR-34c-5p mimic reduced the luciferase activity of MMP2-AS1 and MMP2 3 ′ UTR. The depletion of MMP2-AS1 enhanced miR-34c-5p expression and the expression of MMP2 was inhibited by miR-34c-5p in RCC cells. The protein levels of MMP2 were downregulated by MMP2-AS1 knockdown, while the inhibitor of miR-34c-5p rescued the expression of MMP2 in the cells. The treatment of miR-34c-5p mimic attenuated the cell viability, proliferation, invasion, and migration of RCC cells, in which MMP2 overexpression restored the phenotypes. MMP2-AS1 depletion-attenuated viability, proliferation, migration, and invasion of RCC cells were reversed by miR-34c-5p inhibitor. We concluded that MMP2-AS1 contributed to progression of renal cell carcinoma by modulating miR-34c-5p/MMP2 axis.

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“…Interestingly, the same miRNA may act as a tumor-promoting gene or a tumor suppressor gene in different cancers. For example, miR-34c-5p inhibits the proliferation, migration, and invasion of osteosarcoma cells by targeting FLOT2 (34), and suppresses the metastasis and invasion of cervical cancer by targeting Notch 1 (35). However, miR-34c-5p can also promote colon cancer cell proliferation by targeting SIRT6 via activation of the JAK2/STAT3 signaling pathway (36).…”

Section: Discussionmentioning

confidence: 99%

miR-34c-5p mediates the cellular malignant behaviors of oral squamous cell carcinoma through targeted binding of TRIM29

Shen¹,

Sun²,

Zhao³

et al. 2021

Ann Transl Med

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miR‑34c‑5p targets Notch1 and suppresses the metastasis and invasion of cervical cancer

Cited by 17 publications

References 35 publications

MiR-26a-5p regulates proliferation, apoptosis, migration and invasion via inhibiting hydroxysteroid dehydrogenase like-2 in cervical cancer cell

MiR-26a-5p regulates proliferation, apoptosis, migration and invasion via inhibiting hydroxysteroid dehydrogenase like-2 in cervical cancer cell

Long Noncoding RNA MMP2-AS1 Contributes to Progression of Renal Cell Carcinoma by Modulating miR-34c-5p/MMP2 Axis

miR-34c-5p mediates the cellular malignant behaviors of oral squamous cell carcinoma through targeted binding of TRIM29

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