MiR-367 regulates cell proliferation and metastasis by targeting metastasis-associated protein 3 (MTA3) in clear-cell renal cell carcinoma

Ding, Dexin; Zhang, Yue; Wen, Lin; Fu, Jiangbo; Bai, Xue; Fan, Yuhua; Yuan, Lin; Dai, Hongshuang; Li, Qiang; Zhang, Yong; An, Ruihua

doi:10.18632/oncotarget.18647

Cited by 24 publications

(23 citation statements)

References 36 publications

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“…MTA3 presented the highest fold change in normal and glioma tissues (Figure B). MTA3 has been reported to be a tumor inhibitor in several tumors, including glioma . Thus, we chose MTA3 for subsequent experiments.…”

Section: Resultsmentioning

confidence: 99%

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Long non‐coding RNA HCG11 suppresses the growth of glioma by cooperating with the miR‐4425/MTA3 axis

Zhang

Cao

Kou

et al. 2019

The Journal of Gene Medicine

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Background Glioma is a type of malignant tumor that occurs in the central nervous system of adults. Long non‐coding RNAs (lncRNAs) that potentially participate in the initiation and progression of glioma have been widely reported. As a now‐found lncRNA, HLA complex group 11 (HCG11) has not yet been studied in glioma. The present study aimed to determine the role of HCG11 in the tumorigenesis of glioma. Methods A quantitative real‐time polymerase chain reaction assay was performed to examine the expression pattern of HCG11 in 84 glioma tissues and cell lines. The overall survival rate of glioma patients with a high or low level of HCG11 or metastasis‐associated 1 family member 3 (MTA3) was analyzed by Kaplan–Meier analysis. The effect of HCG11 on glioma cell growth was determined by in vitro and in vivo experiments. MicroRNAs (miRNAs) that potentially interact with HCG11 were searched and determined by bioinformatics analysis and a luciferase reporter assay. Similarly, the target of miRNA‐4425 was identified. Finally, rescue assays were conducted to determine the bio‐function of the competing endogenous RNA pathway. Results HCG11 was downregulated in 84 pairs of glioma tissues and cell lines. Moreover, a low level of HCG11 indicted the lower overall survival rate of glioma patients. Regarding the mechanism, HCG11 was abundant in the cytoplasm of glioma cells and interacted with miR‐4425 to release the expression of MTA3. miR‐4425 and MTA3 participated in HCG11‐mediated glioma growth. Conclusions LncRNA HCG11 suppresses the growth of glioma by cooperating with the miR‐4425/MTA3 axis.

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Section: Resultsmentioning

confidence: 99%

“…MTA3 has been reported to be a tumor inhibitor in several tumors, including glioma. [21][22][23][24] Thus, we chose MTA3 for subsequent experiments.…”

Section: Mta3 Is a Target Of Mir-4425 In Gliomamentioning

confidence: 99%

Long non‐coding RNA HCG11 suppresses the growth of glioma by cooperating with the miR‐4425/MTA3 axis

Zhang

Cao

Kou

et al. 2019

The Journal of Gene Medicine

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“…Our study confirmed the role for miR‐367‐3p as a tumour suppressive miRNA in cervical cancer. However, there are also studies reporting an oncogenic function of miR‐367‐3p with respect to tumour progression . These findings report a disparate function for miR‐367‐3p in tumour progression and indicate that the precise expression status and function of miR‐367‐3p may be dependent on the type of tumour involved.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‐367‐3p overexpression represses the proliferation and invasion of cervical cancer cells through downregulation of SPAG5‐mediated Wnt/β‐catenin signalling

Yang

Tian

Wang

et al. 2020

Clin Exp Pharma Physio

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MicroRNA‐367‐3p (miR‐367‐3p) has been previously reported as a cancer‐related miRNA that is dysregulated in various cancer types and functions either as an oncogenic or as tumour suppressive miRNA. However, whether miR‐367‐3p is dysregulated in cervical cancer and, further, whether it contributes to the development and progression of the disease remains unknown. Here, our results demonstrated that miR‐367‐3p expression was markedly decreased in both cervical cancer tissues and cell lines compared with corresponding controls. In vitro experiments revealed that miR‐367‐3p overexpression repressed the proliferation and invasion of cervical cancer cells. Notably, sperm‐associated antigen 5 (SPAG5) was identified as a target gene of miR‐367‐3p. Moreover, decreased expression of miR‐367‐3p was correlated with high expression of SPAG5 in cervical cancer tissue specimens. SPAG5 inhibition or miR‐367‐3p overexpression significantly downregulated Wnt/β‐catenin signalling in cervical cancer cells. However, the antitumour effect mediated by miR‐367‐3p overexpression was partially reversed by SPAG5 overexpression. Overall, these findings demonstrate that miR‐367‐3p overexpression restricts the proliferation and invasion of cervical cancer cells through targeting SPAG5 to downregulate Wnt/β‐catenin signalling, suggesting a mechanism for the tumour suppressive function of miR‐367‐3p in cervical cancer. Our study highlights the involvement of miR‐367‐3p/SPAG5/Wnt/β‐catenin signalling axis in regulating the malignant progression of cervical cancer.

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“…A number of recent studies have identified a variety of deregulated miRNAs in ccRCC, including miR-224 (27), miR-502 (28), miR-543 (18) and miR-645 (29). miRNAs are involved in several biological and pathological processes, including ccRCC occurrence and development (30)(31)(32). Previous studies have also identified several deregulated miRNAs in ccRCC, whereby oncogenic miRNAs are upregulated and tumor suppressor miRNAs are downregulated (17,33).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‑508 is downregulated in clear cell renal cell carcinoma and targets ZEB1 to suppress cell proliferation and invasion

Wang

et al. 2019

Exp Ther Med

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Recent studies have identified several microRNAs (miRNAs/miRs) that are dysregulated in clear cell renal cell carcinoma (ccRCC), and their dysregulation may serve important roles in the occurrence and development of ccRCC. Therefore, understanding the expression pattern and functional roles of miRNAs in ccRCC may facilitate the identification of novel therapeutic targets for the treatment of ccRCC. In the current study, reverse transcription-quantitative polymerase chain reaction was used to determine miR-508 expression levels in ccRCC tissue samples and cell lines. The cell counting kit-8 and in vitro Transwell invasion assays were used to examine the effects of miR-508 overexpression on ccRCC cell proliferation and invasion, respectively. In addition, bioinformatics analysis and dual-luciferase reporter gene assays were used to investigate the underlying mechanism of miR-508 in ccRCC cells. Furthermore, the regulatory role of miR-508 on zinc finger E-box-binding homeobox 1 (ZEB1) mRNA and protein expression in ccRCC cells was investigated using RT-qPCR and western blot analysis, respectively. Additionally, the association between miR-508 and ZEB1 expression in ccRCC tissue samples was examined. Rescue experiments were performed to determine whether the tumor suppressive effects of miR-508 may be mediated by ZEB1 in ccRCC cells. The results of the current study demonstrated that miR-508 expression was significantly downregulated in ccRCC tissue samples and cell lines. In addition, miR-508 overexpression significantly decreased the proliferation and invasion of ccRCC cells. ZEB1 was identified as a direct target gene of miR-508 in ccRCC cells and the relative expression level of ZEB1 mRNA was significantly increased in ccRCC tissue samples. Furthermore, a negative correlation between miR-508 and ZEB1 expression was identified in ccRCC tissues. ZEB1 knockdown exhibited a functional role similar to miR-508 overexpression in ccRCC cells, and restoration of ZEB1 expression significantly reversed the inhibitory effects of miR-508 on the malignant phenotype of ccRCC cells. Taken together, the results of the current study demonstrated that miR-508 may serve a tumor suppressive role in ccRCC via direct targeting of ZEB1. MiR-508 may present a novel and efficient therapeutic target for the treatment of patients with ccRCC.

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MiR-367 regulates cell proliferation and metastasis by targeting metastasis-associated protein 3 (MTA3) in clear-cell renal cell carcinoma

Cited by 24 publications

References 36 publications

Long non‐coding RNA HCG11 suppresses the growth of glioma by cooperating with the miR‐4425/MTA3 axis

Long non‐coding RNA HCG11 suppresses the growth of glioma by cooperating with the miR‐4425/MTA3 axis

MicroRNA‐367‐3p overexpression represses the proliferation and invasion of cervical cancer cells through downregulation of SPAG5‐mediated Wnt/β‐catenin signalling

MicroRNA‑508 is downregulated in clear cell renal cell carcinoma and targets ZEB1 to suppress cell proliferation and invasion

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