MiR-378 suppresses prostate cancer cell growth through downregulation of MAPK1 in vitro and in vivo

Chen, Qiguang; Zhou, Wei; Han, Tao; Du, Shanshan; Li, Zhenhua; Zhang, Zhe; Shan, Guang-yi; Kong, Chuize

doi:10.1007/s13277-015-3996-8

Cited by 60 publications

(54 citation statements)

References 22 publications

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“…Previous studies showed that miR-378 inhibits prostatic carcinoma cell proliferation 8 and enhanced apoptosis of cardiomyocytes by reduced signaling in the Akt cascade. 38 Additionally, miR-378-5p could suppress cell proliferation and promote cell apoptosis in CRC cells.…”

Section: Discussionmentioning

confidence: 99%

“…7 Specifically, miR-378 can enhance tumor cell survival by repressing SuFu and Fus-1, 24 increase the transcriptional activity of MyoD in part by repressing MyoR, 13 promote BMP2-induced osteogenic differentiation, 17 and also regulate osteoblast differentiation by targeting GalNT-7 in MC3T3-E1 cells. 21 Previous studies also demonstrated that miR-378 suppressed cell migration and promoted cell apoptosis in prostate cancer 8 and increased the size of lipid droplets and the incorporation of acetate into triacylglycerol.…”

Section: Introductionmentioning

confidence: 94%

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miR-378a-3p promotes differentiation and inhibits proliferation of myoblasts by targeting HDAC4 in skeletal muscle development

Wei

Zhang

et al. 2016

RNA Biology

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Muscle development, or myogenesis, is a highly regulated, complex process. A subset of microRNAs (miRNAs) have been identified as critical regulators of myogenesis. Recently, miR-378a was found to be involved in myogenesis, but the mechanism of how miR-378a regulates the proliferation and differentiation of myoblasts has not been determined. We found that miR-378a-3p expression in muscle was significantly higher than in other tissues, suggesting an important effect on muscle development. Overexpression of miR-378a-3p increased the expression of MyoD and MHC in C2C12 myoblasts both at the level of mRNA and protein, confirming that miR-378a-3p promoted muscle cell differentiation. The forced expression of miR-378a-3p promoted apoptosis of C2C12 cells as evidenced by CCK-8 assay and Annexin V-FITC/PI staining results. Through TargetScan, histone acetylation enzyme 4 (HDAC4) was identified as a potential target of miR-378a-3p. We confirmed targeting of HDAC4 by miR-378a-3p using a dual luciferase assay and western blotting. Our RNAi analysis results also showed that HDAC4 significantly promoted differentiation of C2C12 cells and inhibited cell survival through Bcl-2. Therefore, we conclude that miR-378a-3p regulates skeletal muscle growth and promotes the differentiation of myoblasts through the post-transcriptional down-regulation of HDAC4.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%

miR-378a-3p promotes differentiation and inhibits proliferation of myoblasts by targeting HDAC4 in skeletal muscle development

Wei

Zhang

et al. 2016

RNA Biology

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“…The MAPK pathway is well-known to be associated with tumor progression [9] and its upregulation is associated with prostate cancer too [10]. In fact, it has been shown that miR-378 which targets MAPK1 is down-regulated in PCa cells [11]. In addition it has been shown that the Her2/Raf-1/MAPK/AP-1 axis promotes castrate resistant prostate cancer [10].…”

Section: Discussionmentioning

confidence: 99%

The RNA-Binding Protein PCBP1 Functions as a Tumor Suppressor in Prostate Cancer by Inhibiting Mitogen Activated Protein Kinase 1

Zhang

Meng

Xiao

et al. 2018

Cell Physiol Biochem

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Background/Aims: Poly r(C) binding protein (PCBP) 1 or heterogeneous ribonucleoprotein (hnRNP) E1 is a RNA binding protein functional in multiple biological processes. In prostate cancer (PCa), PCBP1 loss was shown to be involved with increased stemness in PCacells; however, the underlying mechanism remains unclear. Method: The role of PCBP1 in prostate tumor formationwas determined by xenograft assays. Immunoprecipitationand mass spectrometry were performed to find the pathways altered after PCBP1 knockdown. Cell proliferation, migration, invasion, and soft agar colony formationassays and xenograft assays were used to determine the role of target protein pathogenesis regulation and formation of PCa. QRT-PCR was performedto quantify relative mRNA expression. Results: The expression of mitogen activated protein kinase 1 (MAPK1) or extracellular signal regulated kinase 2 (ERK2) was increased following PCBP1 loss. Attenuation of MAPK1 inhibited in vitro and in vivo tumorigenicity and metastasis in PCa cell line, PC3. Overexpression of MAPK1 in the PC3 cells increased the tumorigenicity and metastasis. Analysis of PCBP1 and MAPK1 mRNA levels in 25 PCa patients compared to tumor-adjacent normal tissue confirmed an inverse correlation between PCBP1 and MAPK1 expression. Conclusions: PCBP1 can act as a suppressor of tumor in prostate epithelial cells by inhibiting MAPK1 expression.

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“…One study conducted by Bo Hu demonstrated that miR‐212‐3p has an antitumour role in PCa by means of targeting MAPK1. MAPK1, which belongs to the MAPK family, is closely associated with PCa, especially in the aspects of cell proliferation, cell cycle and apoptosis . Ji‐ping Zeng demonstrated that miR‐212‐3p suppressed GC growth by modulating P21 CIP1 and P27 kip1 .…”

Section: The Role Of Mir‐212 In Cancermentioning

confidence: 99%

“…MAPK1, which belongs to the MAPK family, is closely associated with PCa, especially in the aspects of cell proliferation, cell cycle and apoptosis. 38 Ji-ping Zeng demonstrated that miR-212-3p suppressed GC growth by modulating P21 CIP1 and P27 kip1 . 39 It was verified that P21 CIP1 and P27 kip1 are associated with cell cycle arrest, and they showed a tendency for lower expression in GC.…”

Section: Mir-212 and The Cell Cyclementioning

confidence: 99%

The functions and targets of miR‐212 as a potential biomarker of cancer diagnosis and therapy

Song

Bian

Yang

et al. 2020

J Cellular Molecular Medi

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Cancer is a major health problem worldwide. An increasing number of researchers are studying the diagnosis, therapy and mechanisms underlying the development and progression of cancer. The study of noncoding RNA has attracted a lot of attention in recent years. It was found that frequent alterations of miRNA expression not only have various functions in cancer but also that miRNAs can act as clinical markers of diagnosis, stage and progression of cancer. MiR‐212 is an important example of miRNAs involved in cancer. According to recent studies, miR‐212 may serve as an oncogene or tumour suppressor by influencing different targets or pathways during the oncogenesis and the development and metastasis of cancer. Its deregulation may serve as a marker for the diagnosis or prognosis of cancer. In addition, it was recently reported that miR‐212 was related to the sensitivity or resistance of cancer cells to chemotherapy or radiotherapy. Here, we summarize the current understanding of miR‐212 functions in cancer by describing the relevant signalling pathways and targets. The role of miR‐212 as a biomarker and its therapeutic potential in cancer is also described. The aim of this review was to identify new methods for the diagnosis and treatment of human cancers.

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MiR-378 suppresses prostate cancer cell growth through downregulation of MAPK1 in vitro and in vivo

Cited by 60 publications

References 22 publications

miR-378a-3p promotes differentiation and inhibits proliferation of myoblasts by targeting HDAC4 in skeletal muscle development

miR-378a-3p promotes differentiation and inhibits proliferation of myoblasts by targeting HDAC4 in skeletal muscle development

The RNA-Binding Protein PCBP1 Functions as a Tumor Suppressor in Prostate Cancer by Inhibiting Mitogen Activated Protein Kinase 1

The functions and targets of miR‐212 as a potential biomarker of cancer diagnosis and therapy

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