miR-409-3p inhibits HT1080 cell proliferation, vascularization and metastasis by targeting angiogenin

Weng, Chunhua; Dong, Haojie; Chen, Guangdi; Zhai, Yixing; Bai, Rongpan; Hu, Hu; Lu, Linrong; Xu, Zhengping

doi:10.1016/j.canlet.2012.04.010

Cited by 59 publications

(51 citation statements)

References 36 publications

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“…MiR-409-3p is a recently discovered miRNA, which has not yet been extensively researched in human cancer. To the best of our knowledge, it has been reported to be dysregulated only in gastric cancer Zheng et al, 2012) and fibrosarcoma (Weng et al, 2012). In the present study, the miR-409-3p expression level was significantly lower in bladder cancer cells and tissues compared with SV-HUC-1 and corresponding nontumorous tissues, respectively.…”

Section: C-met Promotes the Migration And Invasion Of Bladder Cancer supporting

confidence: 37%

MicroRNA-409-3p Inhibits Migration and Invasion of Bladder Cancer Cells via Targeting c-Met

Chen

Lin

et al. 2013

Molecules and Cells

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There is increasing evidence suggesting that dysregulation of certain microRNAs (miRNAs) may contribute to tumor progression and metastasis. Previous studies have shown that miR-409-3p is dysregulated in some malignancies, but its role in bladder cancer is still unknown. Here, we find that miR-409-3p is down-regulated in human bladder cancer tissues and cell lines. Enforced expression of miR-409-3p in bladder cancer cells significantly reduced their migration and invasion without affecting cell viability. Bioinformatics analysis identified the pro-metastatic gene c-Met as a potential miR-409-3p target. Further studies indicated that miR-409-3p suppressed the expression of cMet by binding to its 3′-untranslated region. Silencing of cMet by small interfering RNAs phenocopied the effects of miR-409-3p overexpression, whereas restoration of c-Met in bladder cancer cells bladder cancer cells overexpressing miR-409-3p, partially reversed the suppressive effects of miR-409-3p. We further showed that MMP2 and MMP9 may be downstream effector proteins of miR-409-3p. These findings indicate that miR-409-3p could be a potential tumor suppressor in bladder cancer.

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Section: C-met Promotes the Migration And Invasion Of Bladder Cancer supporting

confidence: 37%

MicroRNA-409-3p Inhibits Migration and Invasion of Bladder Cancer Cells via Targeting c-Met

Chen

Lin

et al. 2013

Molecules and Cells

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“…Many of the bovine miRNAs differentially expressed in large atretic follicles are reportedly involved in regulating the proliferation and survival of different cell types (miR-483, miR-150, miR-21, miR-409a, miR-31, miR-34b, miR-33a; Yamakuchi et al 2008, Bertero et al 2011, Liu et al 2011, Brabletz 2012, Cirera-Salinas et al 2012, Weng et al 2012 as well as immune cell activation (miR-150, miR-21, miR-31; Xiao et al 2007, Xue et al 2013, Das et al 2014, which is consistent with an active role of these miRNAs in follicular fate decisions. The involvement of miR-21 in the promotion of cell survival is well established, including in mouse granulosa cells during ovulation (Carletti et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Characterization of microRNAs differentially expressed during bovine follicle development

Sontakke¹,

Mohammed²,

McNeilly³

et al. 2014

Reproduction

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Several different miRNAs have been proposed to regulate ovarian follicle function; however, very limited information exists on the spatiotemporal patterns of miRNA expression during follicle development. The objective of this study was to identify, using microarray, miRNA profiles associated with growth and regression of dominant-size follicles in the bovine monovular ovary and to characterize their spatiotemporal distribution during development. The follicles were collected from abattoir ovaries and classified as small (4-8 mm) or large (12-17 mm); the latter were further classified as healthy or atretic based on estradiol and CYP19A1 levels. Six pools of small follicles and individual large healthy (nZ6) and large atretic (nZ5) follicles were analyzed using Exiqon's miRCURY LNA microRNA Array 6th gen, followed by qPCR validation. A total of 17 and 57 sequences were differentially expressed (greater than or equal to twofold; P!0.05) between large healthy and each of small and large atretic follicles respectively. Bovine miRNAs confirmed to be upregulated in large healthy follicles relative to small follicles (bta-miR-144, bta-miR-202, bta-miR-451, bta-miR-652, and bta-miR-873) were further characterized. Three of these miRNAs (bta-miR-144, bta-miR-202, and bta-miR-873) were also downregulated in large atretic follicles relative to large healthy follicles. Within the follicle, these miRNAs were predominantly expressed in mural granulosa cells. Further, body-wide screening revealed that bta-miR-202, but not other miRNAs, was expressed exclusively in the gonads. Finally, a total of 1359 predicted targets of the five miRNAs enriched in large healthy follicles were identified, which mapped to signaling pathways involved in follicular cell proliferation, steroidogenesis, prevention of premature luteinization, and oocyte maturation.

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“…Moreover, MVs derived from mesenchymal stem cells under conditions of hypoxia or serum deprivation contain ANG, which mainly contributes to the angiogenesis-promoting functions of the MVs [130]. ANG can also induce vasculogenic mimicry of fibrosarcoma HT1080 cells [131]. These studies suggested that ANG could facilitate tumor angiogenesis or/and vasculogenic mimicry to permit tumor cell metastases through the blood vessels.…”

Section: Ang and Tumorigenesismentioning

confidence: 97%

Three decades of research on angiogenin: a review and perspective

Sheng¹,

Xu²

2016

ABBS

Self Cite

181

201

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As a member of the vertebrate-specific secreted ribonucleases, angiogenin (ANG) was first isolated and identified solely by its ability to induce new blood vessel formation, and now, it has been recognized to play important roles in various physiological and pathological processes through regulating cell proliferation, survival, migration, invasion, and/or differentiation. ANG exhibits very weak ribonucleolytic activity that is critical for its biological functions, and exerts its functions through activating different signaling transduction pathways in different target cells. A series of recent studies have indicated that ANG contributes to cellular nucleic acid metabolism. Here, we comprehensively review the results of studies regarding the structure, mechanism, and function of ANG over the past three decades. Moreover, current problems and future research directions of ANG are discussed. The understanding of the function and mechanism of ANG in a wide context will help to better delineate its roles in diseases, especially in cancer and neurodegenerative diseases.

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miR-409-3p inhibits HT1080 cell proliferation, vascularization and metastasis by targeting angiogenin

Cited by 59 publications

References 36 publications

MicroRNA-409-3p Inhibits Migration and Invasion of Bladder Cancer Cells via Targeting c-Met

MicroRNA-409-3p Inhibits Migration and Invasion of Bladder Cancer Cells via Targeting c-Met

Characterization of microRNAs differentially expressed during bovine follicle development

Three decades of research on angiogenin: a review and perspective

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