miR-422a Inhibits Glioma Proliferation and Invasion by Targeting IGF1 and IGF1R

Wang, Haiyang; Tang, Chongyang; Meng, Na; Ma, Wei; Jiang, Zhenfeng; Gu, Yifei; Ma, Guizhen; Ge, Haitao; Shen, Hong; Lin, Zhiguo

doi:10.3727/096504016x14732772150389

Cited by 31 publications

(29 citation statements)

References 19 publications

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“…Previous studies from our group and other groups have shown that miR-422a is abnormally expressed in GBM and may act as a tumor suppressor in GBM (35,38,46). Unlike common screening microarrays in which tumor tissues and ANT are selected randomly, we selected 3 paired GBM tissues with low miR-422a expression and not regulated by methylation.…”

Section: Construction Of the Circrna Network In Gbmmentioning

confidence: 99%

“…In recent years, our group has focused on the role of brain-enriched miRNAs, such as miR-422a, in GBM (35). Other groups have shown that miR-422a plays crucial roles in head and neck squamous cell carcinoma, squamous cell lung cancer, and glioma pathology (36)(37)(38). However, very little is known about miRNAs related to the upstream regulators of GBM; these miRNAs may block tumorigenesis and function as molecular targets in GBM treatment.…”

Section: Introductionmentioning

confidence: 99%

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CircNT5E Acts as a Sponge of miR-422a to Promote Glioblastoma Tumorigenesis

et al. 2018

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Circular RNA and long noncoding RNA function as efficient miRNA sponges that regulate gene expression in eukaryotes. However, the sponges of functional miRNAs in glioblastoma remain largely unknown. Here, we identify a subset of circRNAs and lncRNAs that are specifically increased in miR-422a-downregulated glioblastoma tissues. We characterized a novel circRNA derived from NT5E, named circNT5E, that is regulated by ADARB2 binding to sites flanking circRNA-forming introns. We hypothesized that circNT5E may serve as a sponge against miR-422a in glioblastoma tumorigenesis. circNT5E controlled multiple pathologic processes, including cell proliferation, migration, and invasion. circNT5E directly bound miR-422a and inhibited miR-422a activity. Furthermore, circNT5E was observed to sponge other miRNAs, exhibiting tumor suppressor-like features in glioblastoma. Taken together, these findings highlight a novel oncogenic function of circRNA in glioblastoma tumorigenesis. Microarray profiling of circRNA/lncRNA/mRNA in glioblastoma identifies circNT5E as an oncogenic circular RNA and a sponge of miR-422a. .

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Section: Construction Of the Circrna Network In Gbmmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

CircNT5E Acts as a Sponge of miR-422a to Promote Glioblastoma Tumorigenesis

et al. 2018

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“…miRs execute their functions by binding to sequences in 3′‐untranslated regions (UTR) of target mRNAs (Anglicheau, Muthukumar, & Suthanthiran, ). Several miRs have been described in relation to glioma migration and invasion, activating or inhibiting different pathways (Cui et al, ; Wang et al, ; Zhu, Zhao, Rao, & Xu, ).…”

Section: Malignant Alterations Of Glioma Cells Increase Their Invasionmentioning

confidence: 99%

Glioma infiltration and extracellular matrix: key players and modulators

Ferrer

Moura‐Neto

Mentlein

2018

Glia

166

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An outstanding characteristic of gliomas is their infiltration into brain parenchyma, a property that impairs complete surgical resection; consequently, these tumors might recur, resulting in a high mortality rate. Gliomas invade along preferential routes, such as those along white matter tracts and in the perineuronal and perivascular spaces. Brain extracellular components and their partners and modulators play a crucial role in glioma cell invasion. This review presents an extensive survey of the literature, showing how the brain extracellular matrix (ECM) is modulated during the glioma infiltration process. We explore aspects of ECM interaction with glioma cells, reviewing the main glycosaminoglycans, glycoproteins and proteoglycans. We discuss the roles of ECM-binding proteins, including CD44, RHAMM, integrins and axonal guidance molecules, and highlight the role of proteases and glycosidases in glioma infiltration; in binding and release chemokines, cytokines and growth factors; and in generating new bioactive ECM fragments. We also consider the roles of cytoskeletal signaling, angiogenesis, miRNAs and the glial-to-mesenchymal transition linked to glioma invasion. We closely discuss therapeutic approaches based on the modulation of the extracellular matrix, targeting the control of glioma infiltration, its relative failure in clinical trials, and potential means to overcome this difficulty.

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“…Accumulating studies in the past decades have proved that miRNAs can act as tumor suppressors or cancer‐promoting factors in the development of HCC . miR‐422a is abnormally expressed in colorectal cancer, glioma, head and neck squamous cell carcinoma, and counts in the proliferation and metastasis of cancer cells . Importantly, miR‐422a is downregulated in HCC, and overexpression can significantly inhibit the malignant phenotypes of HCC cells .…”

Section: Introductionmentioning

confidence: 99%

“…18,19 miR-422a is abnormally expressed in colorectal cancer, glioma, head and neck squamous cell carcinoma, and counts in the proliferation and metastasis of cancer cells. [20][21][22] Importantly, miR-422a is downregulated in HCC, and overexpression can significantly inhibit the malignant phenotypes of HCC cells. 23 While the mechanism of miR-422a dysregulation in HCC needs further investigation.…”

Section: Introductionmentioning

confidence: 99%

Long noncoding RNA DUXAP8 contributes to the progression of hepatocellular carcinoma via regulating miR‐422a/PDK2 axis

et al. 2020

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Background Hepatocellular carcinoma (HCC) is one of the most deadly cancer worldwide. Multiple long noncoding RNAs (lncRNAs) are recently identified as crucial oncogenic factors or tumor suppressors. In this study, we explored the functon and mechanism of lncRNA double homeobox A pseudogene 8 (DUXAP8) in the progression of HCC. Methods Expression levels of DUXAP8 in HCC tissue samples were measured using qRT‐PCR. The association between pathological indexes and the expression of DUXAP8 was also analyzed. Human HCC cell lines SMMC‐7721 and QSG‐7701 were used in in vitro studies. CCK‐8 assay was used to assess the effect of DUXAP8 on HCC cell line proliferation. Scratch healing assay and Transwell assay were conducted to detect the effect of DUXAP8 on migration and invasion. Furthermore, dual‐luciferase reporter assay was used to confirm targeting relationship between miR‐422a and DUXAP8. Additionally, Western blot was used to detect the regulatory function of DUXAP8 on pyruvate dehydrogenase kinase 2 (PDK2). Results DUXAP8 expression HCC clinical samples was significantly increased and this was correlated with unfavorable pathological indexes. High expression of DUXAP8 was associated with shorter overall survival time of patients. Its overexpression remarkably facilitated the proliferation, metastasis, and epithelial‐mesenchymal transition of HCC cells. Accordingly, knockdown of it suppressed the malignant phenotypes of HCC cells. Overexpression of DUXAP8 significantly reduced the expression of miR‐422a by sponging it, but enhanced the expression of PDK2. Conclusions DUXAP8 was a sponge of tumor suppressor miR‐422a in HCC, enhanced the expression of PDK2 indirectly, and functioned as an oncogenic lncRNA.

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miR-422a Inhibits Glioma Proliferation and Invasion by Targeting IGF1 and IGF1R

Cited by 31 publications

References 19 publications

CircNT5E Acts as a Sponge of miR-422a to Promote Glioblastoma Tumorigenesis

CircNT5E Acts as a Sponge of miR-422a to Promote Glioblastoma Tumorigenesis

Glioma infiltration and extracellular matrix: key players and modulators

Long noncoding RNA DUXAP8 contributes to the progression of hepatocellular carcinoma via regulating miR‐422a/PDK2 axis

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