miR-429 Identified by Dynamic Transcriptome Analysis Is a New Candidate Biomarker for Colorectal Cancer Prognosis

Sun, Yingnan; Shen, Shourong; Tang, Hailin; Xiang, Juanjuan; Peng, Ya; Tang, Anliu; Li, Nan; Zhou, Weiwei; Wang, Zeyou; Zhang, Decai; Xiang, Bo; Ge, Jie; Li, Guiyuan; Wu, Minghua; Li, Xiayu

doi:10.1089/omi.2012.0132

Cited by 37 publications

(32 citation statements)

References 43 publications

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“…Dual roles of miR-429 have been suggested in a variety of types of human cancer, including ovarian (12), colorectal (13)(14)(15), esophageal (16) and gastric cancer (17,18), as well as osteosarcoma (19). Yoneyama et al (20) demonstrated that the expression level of miR-429, accompanied with miR-200a/b, was significantly increased in endometrioid endometrial carcinoma (ECC) compared to adjacent normal endometrial tissues.…”

Section: Discussionmentioning

confidence: 99%

miR-429 promotes the proliferation of non-small cell lung cancer cells via targeting DLC-1

Xiao

Zhou

2016

Oncology Letters

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Section: Discussionmentioning

confidence: 99%

miR-429 promotes the proliferation of non-small cell lung cancer cells via targeting DLC-1

Xiao

Zhou

2016

Oncology Letters

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“…Most functional studies of MIR429 have reported that MIR429 plays an oncogenic role in the cellular processes of CRC 17 and that MIR429 is significantly down-regulated in stage II and stage III clinical CRC progression. 15 We used mRNA microarray analysis and public bioinformatics tools to identify MIR429 target genes. Of the 168 genes identified, Table 1] based on microarray analysis and bioinformatics tools.…”

Section: Discussionmentioning

confidence: 99%

“…15 In oesophageal carcinomas [EC], MIR429 expression levels are down-regulated, and up-regulation of MIR429 inhibits invasion and promotes apoptosis in EC cells by targeting BCL2 and SP1. 16 Li and co-workers show that MIR429 is upregulated in human CRC tissues and suggest that overexpression of MIR429 inhibits apoptosis in CRC cells through negative regulation of SOX2 expression.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA 429 Regulates Mucin Gene Expression and Secretion in Murine Model of Colitis

Alam

Kim

et al. 2016

ECCOJC

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Background and Aims: miRNAs are non-coding RNAs that play important roles in the pathogenesis of human diseases by regulating target gene expression in specific cells or tissues. We aimed to detect miRNAs related to ulcerative colitis [UC], identify their target molecules, and analyse the correlation between the miRNAs and their target genes in colorectal cells and dextran sulphate sodium [DSS]-induced mouse colitis. Methods: UC-associated miRNAs were identified by miRNA microarray analysis using DSS-induced colitis and normal colon tissues. The results were validated by quantitative real-time polymerase chain reaction [RT-PCR]. We identified target genes of MIR429, a colitis-associated miRNA, from our screen by comparing the mRNA microarray analysis in MIR429-overexpressed cells with predicted candidate target genes. We constructed luciferase reporter plasmids to confirm the effect of MIR429 on target gene expression. The protein expression of the target genes was measured by western blot,enzyme-linked immunosorbent assay [ELISA] analysis, or immunohistochemistry. Results: We identified 37 DSS-induced colitis associated miRNAs. We investigated MIR429 that is down-regulated in DSS-induced colitis, and identified 41 target genes of MIR429. We show that the myristoylated alanine-rich protein kinase C substrate [MARCKS] is a direct target of MIR429. MARCKS mRNA and protein expression levels are down-regulated by MIR429, and MIR429 regulates the expression of MARCKS and MARCKS-mediated mucin secretion in colorectal cells and DSS-induced colitis. In addition, anti-MIR429 up-regulates MARCKS expression in colorectal cell lines. Conclusion: Our findings suggest that MIR429 modulates mucin secretion in human colorectal cells and mouse colitis tissues by up-regulating of MARCKS expression, thereby making MIR429 a candidate for anti-colitis therapy in human UC.

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“…miRNAs play a vital role in many crucial cellular processes, including apoptosis, differentiation, invasion, and proliferation (32)(33)(34). The abnormal expression of miRNA has been reported in many cancers, including breast (35), gastric (36), colorectal (37), and liver cancers (38), as well as leukemia (39) and lymphoma (40). miR-124 has been described as a brain-specific miRNA in mammals, and may play a role in defining and maintaining neuron-specific characteristics (41,42).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-124 inhibits cell proliferation and migration by regulating SNAI2 in breast cancer

Sun

et al. 2016

Oncology Reports

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Abstract. MicroRNA (miRNA) is a type of endogenous non-coding RNA implicated in various cellular processes. Studies have shown that miR-124 is involved in the malignant progression of cancer, but little is known concerning its potential role in breast cancer. Therefore, the purpose of this study was to conduct a functional analysis of miR-124 in breast cancer, and to identify its target genes in this disease. To this end, we used quantitative real-time PCR to examine the expression level of miR-124 in breast cancer tissue specimens and cell lines. To study the functional significance of miR-124, we overexpressed miR-124 with miR-124 mimics and observed breast cancer cell proliferation, colony formation, migration, and invasion abilities by in vitro cell culture experiments. Target prediction algorithms and luciferase reporter gene assays were used to identify the target genes of miR-124. We also knocked down miR-124 targets using short hairpin RNA (shRNA) constructs, and observed associated breast cancer cell characteristics by in vitro cell culture experiments. We found that miR-124 expression significantly decreased in breast cancer tissues and cells compared to normal tissues and cells. In addition, cell proliferation, colony formation, migration, and invasion were decreased after overexpression of miR-124 in breast cancer cells. Furthermore, we used several algorithms to identify the snail family zinc finger 2 (SNAI2) as a potential target gene of miR-124. The protein expression level and luciferase activity of the 3'-untranslated region of SNAI2 were significantly decreased in breast cancer cells transfected with miR-124 mimics. Cell proliferation, colony formation, migration, and invasion were also decreased after knockdown of SNAI2 by shRNA. In conclusion, our data suggest that miR-124 expression is decreased in breast cancer and plays an important role as a tumor suppressor gene by targeting SNAI2. These findings may reveal novel perspectives for clinical treatments against breast cancer.

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miR-429 Identified by Dynamic Transcriptome Analysis Is a New Candidate Biomarker for Colorectal Cancer Prognosis

Cited by 37 publications

References 43 publications

miR-429 promotes the proliferation of non-small cell lung cancer cells via targeting DLC-1

miR-429 promotes the proliferation of non-small cell lung cancer cells via targeting DLC-1

MicroRNA 429 Regulates Mucin Gene Expression and Secretion in Murine Model of Colitis

MicroRNA-124 inhibits cell proliferation and migration by regulating SNAI2 in breast cancer

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