miR-429 Inhibits Differentiation and Promotes Proliferation in Porcine Preadipocytes

Abstract

MicroRNAs (miRNAs) are crucial regulatory molecules for adipogenesis. They contribute to the controlling of proliferation and differentiation of preadipocytes. Previous studies revealed an important role of miR-429 in cell invasion, migration, and apoptosis. Our previous work has shown that the expression of miR-429 in subcutaneous fat can be observed in newly born (3-day-old) Rongchang piglets rather than their adult counterparts (180-day-old). This expression pattern suggests that miR-429 might be functionally related to postnatal adipogenesis. However, we currently lack a mechanistic understanding of miR-429 within the context of preadipocyte differentiation. In this study, we investigated the function of miR-429 in porcine subcutaneous and intramuscular preadipocyte proliferation and differentiation. In our porcine preadipocyte differentiation model, miR-429 expression decreased remarkably upon adipogenic induction. Overexpression of miR-429 notably down-regulated the expression of adipogenic marker genes: PPARγ, aP2, FAS and impaired the triglyceride accumulation, while the expression of lipolytic gene ATGL was not affected. In addition, we observed that miR-429 significantly promoted the proliferation of porcine preadipocytes. We also found that miR-429 could directly bind to the 3′-UTRs of KLF9 and p27, which have been well documented to promote preadipocyte differentiation and repress cell cycle progression. Taken together, our data support a novel role of miR-429 in regulating porcine preadipocyte differentiation and proliferation, and KLF9 and p27 are potent targets of miR-429 during these processes.

MiR-146a-5p Targeting SMAD4 and TRAF6 Inhibits Adipogenensis Through TGF-β and NF-κB Signal Pathways in Porcine Intramuscular Preadipocytes

“…Generally, a gene or miRNA has the opposite effect on cell proliferation and differentiation. Recent studies showed that miR-664-5p promoted myoblast proliferation and inhibited myoblast differentiation [30], and miR-429 accelerated proliferation of porcine preadipocytes and repressed adipogenic differentiation [31]. Interestingly, in this study, miR-146a-5p repressed both proliferation and differentiation of intramuscular preadipocytes.…”

MTCH2 promotes adipogenesis in intramuscular preadipocytes via an m 6 A‐YTHDF1‐dependent mechanism

“…Visible connective tissue was removed and finely minced. Intramuscular preadipocytes were isolated based on previous studies (11,30,31). In brief, muscle tissues were digested in a digestion buffer consisting of 1 mg/ml collagenase type I (Thermo Fisher Scientific) in a shaking water bath for 1.5 h at 37°C.…”

MicroRNA-125a-5p Affects Adipocytes Proliferation, Differentiation and Fatty Acid Composition of Porcine Intramuscular Fat

“…According to previous reports [ 25 , 46 , 47 ], one female LiangShan Pig was sacrificed for porcine intramuscular preadipocytess isolation at three days. Briefly, Longissimus dorsi muscles (LDM) were aseptically isolated and washed three times using phosphate buffered saline (PBS, Gibco, Grand Island, NY, USA) containing 200 U/mL penicillin–streptomycin (Hyclone, Logan, UT, USA).…”