miR-4324 inhibits ovarian cancer progression by targeting FEN1

Abstract

Background Ovarian cancer is one of the most lethal malignancies, with a 1.9% mortality rate worldwide. The dysregulation of the FEN1 gene and miR-4324 has been associated with cancer progression. However, the relationship between miR-4324 and-FEN1 requires further investigation. Methods miR-4324 and FEN1 expressions in ovarian cancer tissues and cell lines were measured via RT-qPCR. The interaction between miR-4324 and FEN1 was assessed using luciferase and RNA pull-down assays. The effects of miR-4324 and FEN1 on cell proliferation, adhesion and apoptosis were determined by CCK-8, BrdU, colony formation, cell adhesion, Caspase-3 and western blot assays in ovarian cancer cell lines CaOV3 and OVCAR3, respectively. Results The results showed that miR-4324 expression was significantly decreased and FEN1 expression was enhanced in ovarian cancer tissues and cell lines. miR-4324 inhibitor promoted cell proliferation, adhesion and migration, and prevented apoptosis. Furthermore, the downregulation of FEN1 inhibited ovarian cancer cell growth and increased apoptosis. miR-4324 inhibited FEN1 expression and repressed ovarian cancer progression. Conclusion Our study found that miR-4324 inhibited FEN1 expression, suppressed cell growth, and increased apoptosis in ovarian cancer cells. Therefore, we identified miR-4324 and FEN1 as potential therapeutic targets for ovarian cancer treatment.

Whole transcriptome analysis revealed the regulatory network and related pathways of non-coding RNA regulating ovarian atrophy in broody hens

“…Its expression is thus significantly reduced in ovarian cancer tissues. miR-4324 can be targeted and combined with FEN1 to impede cell proliferation in ovarian cancer ( 43 , 44 ). Recent studies postulate that miR-143 has a regulatory effect on glucose metabolism and mitochondrial function in the placenta ( 45 ).…”

The significance of HAUS1 and its relationship with immune microenvironment in hepatocellular carcinoma

“…According to the protocol's description, getting 1000 cells and planting them into each well of the 96-well plates, leaving blank controls. CCK-8 reagent (Cat#: K1018; APExBIO, China) were added once a day for 4 consecutive days after cell culture, and the OD value was measured with the absorbance of 450 nm after 37 °C incubation 26 .…”

CircUBE2D2 regulates HMGB1 through miR-885-5p to promote ovarian cancer malignancy

“… 11 Some miRNAs have been identified to be associated with OC, for example, miR-425-5p and miR-760 promote OC cell proliferation and metastasis, 12 , 13 and miR-143, miR-365 and miR-4324 inhibit metastasis. 14 , 15 , 16 It is known that miR-885-5p can inhibit tumor cell proliferation and metastasis. 17 , 18 , 19 HMGB1 has been measured to be highly expressed in tumor cells 20 , 21 , 22 and is a new potential OC marker, especially for epithelial OC.…”