MiR-433-3p suppresses cell growth and enhances chemosensitivity by targeting CREB in human glioma

Abstract

Previous studies reported that miR-433 exerts function widely in human tumorigenesis and development. Here, we further investigate the potential role of miR-433 in glioma. Quantitative real-time PCR demonstrated that miR-433-3p and miR-433-5p were low expressed in glioma tissues and cell lines. Functional studies suggested that the overexpression of miR-433-3p suppressed proliferation, induced apoptosis and inhibited invasion and migration of human glioma cells. But the growth and metastasis of glioma cells were not significantly influenced by overexpression of miR-433-5p. In a xenograft model, we also showed that miR-433-3p had an inhibitory effect on the growth of glioma. Bioinformatics coupled with luciferase and western blot assays revealed that CREB is a direct target of miR-433-3p, and the overexpression of CREB can rescue the phenotype changes induced by miR-433-3p overexpression. Besides, miR-433-3p could increase chemosensitivity of glioma to temozolomide by targeting CREB in vitro and in vivo. Taken together, these results suggest that miR-433-3p may function as a potential marker in diagnostic and therapeutic target for glioma.

Circular RNA hsa_circ_0008344 regulates glioblastoma cell proliferation, migration, invasion, and apoptosis

“…Increased evidence has confirmed this idea that circular RNA‐ZFR inhibited cell proliferation and promoted apoptosis in gastric cancer by sponging miR‐130a/miR‐107 and modulating PTEN, circ‐ITCH inhibited bladder cancer progression by sponging miR‐17/miR‐224 and regulating p21 and PTEN expression, and hsa_circ_0046701 promoted carcinogenesis by increasing the expression of miR‐142‐3p target ITGB8 in glioma . Furthermore, hsa_circ_0008344 may bind to several miRNAs, such as miR‐433‐3p and miR‐450b‐3p, predicted by bioinformatics, while miR‐433‐3p has been found to suppress cell growth and enhance chemosensitivity by targeting CREB in glioblastoma . Accordingly, it is presumed that hsa_circ_0008344 can sponge tumor suppressor miRNAs followed by disinhibition of the expression of some targeted oncogenic genes, which contributes to glioblastoma progression.…”

MicroRNA‑433 reduces cell proliferation and invasion in non‑small cell lung cancer via directly targeting E2F transcription factor 3

“…Decreased miR-433 expression levels are correlated with distant metastasis and pathological TNM stage in patients with gastric cancer (21). MiR-433 downregulation has also been reported in colorectal cancer (22), hepatocellular carcinoma (23,24), myeloproliferative neoplasms (34), oral squamous cell carcinoma (35), ovarian cancer (36), retinoblastoma (37) and glioma (38). Conversely, miR-433 is overexpressed in osteosarcoma (39).…”

“…Li et al (37) indicated that miR-433 overexpression notably suppresses cell growth and metastasis and promotes cell cycle arrest and apoptosis in retinoblastoma. In addition, Sun et al (38) reported that restoring miR-433 expression prohibits cell proliferation and motility in vitro, induces apoptosis in vitro, reduces tumor growth in vivo and increases the chemosensitivity of cells to temozolomide in vitro and in vivo. However, miR-433 has been identified as an oncogene in osteosarcoma by regulating cell apoptosis and growth both in vitro and in vivo (39).…”

A five‐CpG signature of microRNA methylation in non‐G‐CIMP glioblastoma

“…MiR‐127 was reported to promote GBM cell migration and invasion by targeting tumor‐suppresser gene SEPT7 . MiR‐433 was reported to be commonly dysregulated in GBMs and suppressed glioma cell proliferation, migration, invasion, and enhanced sensitivity to TMZ therapy . Regarding miR‐759 and miR‐1248, no biological or clinical evidences have been reported in cancers so far.…”