MiR-449b inhibits the migration and invasion of colorectal cancer cells through the negative regulation of MMP2

Abstract

Background: An increasing number of miRNAs were confirmed to be involved in the initiation and progression of colorectal cancer (CRC) by acting as cancer suppressor genes and oncogenes. The purpose of this study was to uncover the role of miR-449b in CRC and its underlying mechanisms. Methods: The expression profile of miR-449b in human CRC tissues and cell lines was determined using quantitative real-time polymerase chain reaction (qRT-PCR) analysis. The effect of miR-449b on CRC was assessed by CRC cell migration and invasion as determined using a transwell assay. A luciferase reporter assay was also carried out to explore the interaction between MMP2 3′UTR and miR-449b. Results: The current study revealed that the relative expression of miR-449b was decreased significantly in human CRC tissues and cell lines; meanwhile, miR-449b in metastatic CRC tissues was significantly lower than that in non-metastatic CRC tissues. We also observed that the forced miR-449b in CRC cells significantly restrained cell migration and invasion in vitro. In contrast to miR-449b, our study found that both the MMP-2 mRNA and protein levels were increased in CRC cells. MiR-449b negatively regulated MMP2 in CRC cells, and the result was corroborated by luciferase reporter assay. Most importantly, overexpression of MMP2 significantly reversed the miR-449b-mediated inhibition on the invasion and migration of CRC cells. Conclusion: Our data provided encouraging evidence that miR-449b possessed a strong inhibitory effect on CRC cell migration and invasion by negative regulation of MMP2.

<p>Interfering Human Papillomavirus E6/E7 Oncogenes in Cervical Cancer Cells Inhibits the Angiogenesis of Vascular Endothelial Cells via Increasing miR-377 in Cervical Cancer Cell-Derived Microvesicles</p>

“…The genetic materials encapsulated by MVs, such as microRNAs (miRNAs), can be transferred from tumor cells to adjacent cells, such as endothelial cells, thus affecting the tumor microenvironment. 17,18 For instance, miR-1246 encapsulated by colorectal cancer cellderived MVs can activate PML-induced Smad 1/5/8 signaling in endothelial cells, thereby promoting the proliferation, migration and angiogenesis of endothelial cells. 19 According to miRNA expression analysis in umbilical cord blood MVs using miRNA PCR arrays, miR-377 was detected in MVs from umbilical cord blood, 20 indicating that miR-377 can be carried by MVs.…”

Malignant Transformation of a Mature Ovarian Teratoma: A Case Report and Review of the Literature

“…Currently, the diagnostic effects of the serum tumor markers on the malignant transformation of a teratoma are still controversial [3] . Researchers have also found that the CA125 and CA199 levels were closely related with the diameter of the teratoma and the prognosis of mature ovarian teratomas [13] . In this case, the serum CA199 level was upregulated, and the levels of the other tumor markers were within the normal range.…”

“…Tumors show up as off-white or light yellow and brittle, and they are often accompanied by bleeding and necrosis. A malignant transformation often occurs in the inner papilla of the cyst wall, near the nodules, in the inhomogeneous enhanced portion or the thickening cyst wall [3,4,13] . Therefore, surgeons should carefully examine the cyst wall of the specimen.…”

Interfering with miR-24 alleviates rotenone-induced dopaminergic neuron injury via enhancing autophagy by upregulating DJ-1

“…Although many studies have investigated the role of DJ-1 in PD, the molecular mechanism of DJ-1 in autophagy in PD is not completely understood. MicroRNAs (miRNAs) are a class of noncoding RNAs with a length between 18 and 25 nucleotides, which play an important role in various cellular biology processes [9]. Recently, growing evidence has revealed that multiple miRNAs are involved in the development of PD.…”