miR‑449c inhibits migration and invasion of gastric cancer cells by targeting PFKFB3

Chen, Xin; Wang, Anping; Yue, Xinxia

doi:10.3892/ol.2018.8609

Cited by 6 publications

(8 citation statements)

References 16 publications

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“…It also plays critical roles in adjusting the biological processes of cancer cells. Current evidence suggests that miR-449c can be widely involved in tumor cell growth, migration, proliferation and apoptosis (Miao et al, 2013;Sandbothe et al, 2017;Chen, Wang & Yue, 2018). We found that miR-449c was also upregulated in the HG group.…”

Section: Discussionsupporting

confidence: 47%

Integrative analysis of competitive endogenous RNA network reveals the regulatory role of non-coding RNAs in high-glucose-induced human retinal endothelial cells

Cao

Liu

Dong

et al. 2020

PeerJ

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Background Increasing evidence has suggested that non-coding RNAs (ncRNAs) play critical roles in the pathogenesis of diabetic retinopathy (DR), but their underlying mechanisms remain unclear. The purpose of this study was to determine latent key genes and to structure a competing endogenous RNA (ceRNA) regulatory network to discover the potential molecular mechanisms governing the effects of high glucose on human retinal endothelial cells (HRECs). Methods We obtained microarray data for long non-coding RNA (lncRNA) and mRNA of high-glucose-induced HREC samples from NCBI GEO datasets. The ceRNA network was screened using intersecting prediction results from miRcode, TargetScan, miRTarBase and miRDB. The protein–protein interaction (PPI) network was constructed using the Search Tool for the Retrieval of Interacting Genes and hub genes were obtained using the cytoHubba app. The ClusterProfiler package was applied for performing Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. The expression of key RNAs was verified using the qRT-PCR method. A key ceRNA subnetwork was constructed based on the criticality of the genes and its binding sites were verified by luciferase reporter assay. The viability and apoptosis of HRECs were tested using the transfection of the miR-449c inhibitor. Results A total of 3,328 lncRNAs and 2,017 mRNAs were screened for differentially expressed (DE) profiles. The newly constructed ceRNA network was composed of 410 lncRNAs, 35 miRNAs and 122 mRNAs. The 10 hub genes were identified through the PPI network. GO and KEGG analysis revealed that DE mRNAs were mainly related to the positive regulation of the mRNA catabolic process, cell polarity, and the G1/S transition of mitotic and cell cycle signaling pathways. QRT-PCR was used to verify RNAs and the most important genes were screened out. A key ceRNA subnetwork OIP5-AS1/miR-449c/MYC was established. The binding site was verified by luciferase reporter assay. The expression levels of OIP5-AS1 and MYC increased after miR-449c inhibitor transfection, miR-449c decreased, HRECs activity increased, and apoptosis decreased, compared with the control group. Conclusion We successfully built the key ceRNA subnetwork, OIP5-AS1/miR-449c/MYC, by applying the GEO database for data analysis and mining. The results from the ceRNA network allow us to better understand the effect of ncRNAs on HRECs under hyperglycemic conditions and the pathogenesis of DR.

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Section: Discussionsupporting

confidence: 47%

Integrative analysis of competitive endogenous RNA network reveals the regulatory role of non-coding RNAs in high-glucose-induced human retinal endothelial cells

Cao

Liu

Dong

et al. 2020

PeerJ

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“…In the area of gastric cancer, Feng et al reported that miR-518 suppresses the progression of gastric cancer by promoting cell apoptosis via targeting MDM2 (19). Therefore, miR-518 may be a promising therapeutic target for gastric promotes gastric cancer cell growth and inhibits apoptosis (23). Wang et al demonstrated that over-expression of miR-217 inhibits gastric cancer cell proliferation, invasion and promotes apoptosis via regulating geriatric palliative care 5 (GPC5), and could be a potential therapeutic target (24).…”

Section: Discussionmentioning

confidence: 99%

“…Wang et al showed that overexpression of miR-128b inhibits cell proliferation, migration, and invasion, and promotes apoptosis in gastric cancer cells via down-regulating adenosine 2b receptor (A2bR) ( 22 ). Wu et al reported that up-regulation of miR-449c suppresses gastric cancer cell growth and promotes apoptosis, while down-regulation of miR-449c promotes gastric cancer cell growth and inhibits apoptosis ( 23 ). Wang et al demonstrated that over-expression of miR-217 inhibits gastric cancer cell proliferation, invasion and promotes apoptosis via regulating geriatric palliative care 5 (GPC5), and could be a potential therapeutic target ( 24 ).…”

Section: Discussionmentioning

confidence: 99%

MiR-450a-5p Inhibits Gastric Cancer Cell Proliferation, Migration, and Invasion and Promotes Apoptosis via Targeting CREB1 and Inhibiting AKT/GSK-3β Signaling Pathway

et al. 2021

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Gastric cancer seriously affects human health and research on gastric cancer is attracting more and more attentions. In recent years, molecular targets have become the research focus. Accumulating evidence indicates that miR-450a-5p plays a critical role in cancer progression. However, the biological role of miR-450a-5p in gastric carcinogenesis remains largely unknown. In this study, we explore the effects and mechanisms of miR-450a-5p on the development and progression of gastric cancer. We used gain-of-function approaches to investigate the role of miR-450a-5p on gastric cancer cell proliferation, migration, invasion, and apoptosis using biological and molecular techniques including real-time quantitative PCR (RT-qPCR), CCK-8, colony formation, flow cytometry, Western blot, wound healing, transwell chamber, dual luciferase reporter, and tumor xenograft mouse model. We found that gastric cancer cells have low expression of miR-450a-5p and overexpression of miR-450a-5p inhibited gastric cancer cell proliferation, migration and invasion, and induced apoptosis in vitro. Moreover, we demonstrated that ectopic expression of miR-450a-5p inhibited gastric cancer growth in vivo. At the molecular level, overexpression of miR-450a-5p significantly increased the expression of pro-apoptotic proteins, including caspase-3, caspase-9, and Bax, and inhibited the expression of anti-apoptotic protein Bcl-2. Luciferase reporter experiment suggested that camp response element binding protein 1 (CREB1) had a negative correlation with miR-450a-5p expression, and knockdown of CREB1 alleviated gastric cancer growth. Furthermore, we also found that miR-450a-5p inhibited the activation of AKT/GSK-3β signaling pathway to inhibit the progression of gastric cancer. Collectively, miR-450a-5p repressed gastric cancer cell proliferation, migration and invasion and induced apoptosis through targeting CREB1 by inhibiting AKT/GSK-3β signaling pathway. MiR-450a-5p could be a novel molecular target for the treatment of gastric cancer.

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“…The aberrant expression of miRNAs have been identified to be closely associated to the occurrence of numerous types of human malignant tumor, in which they were reportedly involved in cell apoptosis, proliferation, the cell cycle and metastasis by altering post-transcriptional gene expression ( 20-22 ). miRNAs have been identified to serve as both oncogenes or tumor suppressor genes in tumorigenesis; for example, the abnormal expression of miRNAs, such as miR-146a ( 23 ), miR-375( 24 ) and miR-449c ( 25 ), was determined to be closely associated with gastric tumorigenesis. In addition, a significant association was reported between tumor budding and the downregulated expression levels of miRNAs, such as miR-148a ( 26 ) and miR-625-3p ( 27 ).…”

Section: Discussionmentioning

confidence: 99%

“…Hazard suppressor genes in tumorigenesis; for example, the abnormal expression of miRNAs, such as miR-146a (23), miR-375 (24) and miR-449c (25), was determined to be closely associated with gastric tumorigenesis. In addition, a significant association was reported between tumor budding and the downregulated expression levels of miRNAs, such as miR-148a (26) and miR-625-3p (27).…”

Section: Univariate Cox Analysis Multivariate Cox Analysis ----------mentioning

confidence: 99%

miR‑1225‑5p inhibits non‑small cell lung cancer cell proliferation, migration and invasion, and may be a prognostic biomarker

Zhang

2020

Exp Ther Med

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Non-small cell lung cancer (NSCLC) is a malignant tumor, which presents with a high 5-year mortality rate owing to the lack of an effective early screening tool and the absence of obvious early symptoms. MicroRNAs (miRs/miRNAs) have attracted increasing attention due to their significant clinical value in the diagnosis and prognosis of various human malignancies. The present study aimed to investigate the expression levels of microRNA (miR)-1225-5p in NSCLC and to analyze its prognostic value and biological role. The expression levels of miR-1225-5p in the tissues of patients with NSCLC and NSCLC cell lines were analyzed using reverse transcription-quantitative PCR. The association between miR-1225-5p expression levels and the clinicopathological features of patients with NSCLC was analyzed using a χ 2 test. The prognostic value of miR-1225-5p in NSCLC was analyzed using both Kaplan Meier survival and Cox regression analyses, and the effects of miR-1225-5p on NSCLC cell proliferation, migration and invasion were examined. The results revealed that the expression levels of miR-1225-5p were significantly downregulated in NSCLC tissues compared with normal control tissues. Furthermore, miR-1225-5p was discovered to be a potential independent prognostic factor in NSCLC. The inhibition of miR-1225-5p in NSCLC cell lines led to increased cell proliferation, migration and invasion, whereas miR-1225-5p overexpression exerted the opposite effects in these cells. In conclusion, the findings of the present study indicated that the downregulated expression levels of miR-1225-5p in NSCLC may predict a poor prognosis in patients and suggested miR-1225-5p as a potential therapeutic target for NSCLC.

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miR‑449c inhibits migration and invasion of gastric cancer cells by targeting PFKFB3

Cited by 6 publications

References 16 publications

Integrative analysis of competitive endogenous RNA network reveals the regulatory role of non-coding RNAs in high-glucose-induced human retinal endothelial cells

Integrative analysis of competitive endogenous RNA network reveals the regulatory role of non-coding RNAs in high-glucose-induced human retinal endothelial cells

MiR-450a-5p Inhibits Gastric Cancer Cell Proliferation, Migration, and Invasion and Promotes Apoptosis via Targeting CREB1 and Inhibiting AKT/GSK-3β Signaling Pathway

miR‑1225‑5p inhibits non‑small cell lung cancer cell proliferation, migration and invasion, and may be a prognostic biomarker

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