miR-454 regulates triglyceride synthesis in bovine mammary epithelial cells by targeting PPAR-γ

Zhang, Ming-Qi; Gao, Jun-Lei; Liao, Xian-Dong; Huang, Tung Po; Zhang, Meina; Wang, Ming-Qi; Tian, Yu; Bai, Jian; Zhou, Changhai

doi:10.1016/j.gene.2018.12.048

Cited by 21 publications

(13 citation statements)

References 44 publications

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“…The miR-454 regulates triglyceride synthesis in bovine mammary epithelial cells by targeting PPAR-γ, which may be a crucial factor in enhancing the quality of dairy products (Zhang et al, 2019). Hitherto, the function of miRNA-454 has been explored in several human diseases, and its response to pathogenic infection, however, its role in muscle development has not yet been reported (Wu et al, 2014;Liang et al, 2015;Song et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

MiRNA Profiling in Pectoral Muscle Throughout Pre- to Post-Natal Stages of Chicken Development

Chen

Zhang

et al. 2020

Front. Genet.

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MicroRNA (miRNA) is known to be an important regulator of muscle growth and development. The regulation of microRNA on the skeletal muscle phenotype of animals is mainly achieved by regulating the proliferation and differentiation of myoblasts. In this study, we sequenced a total of 60 samples from 15 developing stages of the pectoral muscle and five other tissues at 300 days of Tibetan chicken. We characterized the expression patterns of miRNAs across muscle developmental stages, and found that the chicken growth and development stage was divided into early-embryonic and late-embryonic as well as postnatal stages. We identified 81 and 21 DE-miRNAs by comparing the miRNA profiles of pectoral muscle of three broad periods and different tissues, respectively; and 271 miRNAs showed time-course patterns. Their potential targets were predicted and used for functional enrichment to understand their regulatory functions. Significantly, GgmiRNA-454 is a time-dependent and tissuedifferential expression miRNA. In order to elucidate the role of gga-miRNA-454 in the differentiation of myoblasts, we cultured chicken myoblasts in vitro. The results show that although gga-miRNA-454-3p initiates increase and thereafter decrease during the chicken myoblasts differentiation, it had no effect on primary myoblasts proliferation. Furthermore, we confirm that gga-miRNA-454 inhibits myoblast differentiation by targeting the myotube-associated protein SBF2.

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Section: Discussionmentioning

confidence: 99%

MiRNA Profiling in Pectoral Muscle Throughout Pre- to Post-Natal Stages of Chicken Development

Chen

Zhang

et al. 2020

Front. Genet.

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“…The PPARγ has been the most studied in dairy cows. In monogastrics this PPAR isotype is a pivotal player in adipogenesis and insulin sensitivity, but in dairy cows it has been specifically studied for its role in modulating milk fat synthesis [248][249][250], its involvement in the mTOR pathway, particularly mTORC2 [251], and its anti-inflammatory role [4,5]. All PPAR isotypes play a role in improving reproductive parameters of dairy cows, such as the mitigation of endometritis [252], contribution to folliculogenesis [253], reproductive hormone synthesis [253], and the development of embryos [254].…”

Section: Peroxisome Proliferator-activated Receptorsmentioning

confidence: 99%

Advances in fatty acids nutrition in dairy cows: from gut to cells and effects on performance

Bionaz

Vargas‐Bello‐Pérez

Busato

2020

J Animal Sci Biotechnol

104

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High producing dairy cows generally receive in the diet up to 5–6% of fat. This is a relatively low amount of fat in the diet compared to diets in monogastrics; however, dietary fat is important for dairy cows as demonstrated by the benefits of supplementing cows with various fatty acids (FA). Several FA are highly bioactive, especially by affecting the transcriptome; thus, they have nutrigenomic effects. In the present review, we provide an up-to-date understanding of the utilization of FA by dairy cows including the main processes affecting FA in the rumen, molecular aspects of the absorption of FA by the gut, synthesis, secretion, and utilization of chylomicrons; uptake and metabolism of FA by peripheral tissues, with a main emphasis on the liver, and main transcription factors regulated by FA. Most of the advances in FA utilization by rumen microorganisms and intestinal absorption of FA in dairy cows were made before the end of the last century with little information generated afterwards. However, large advances on the molecular aspects of intestinal absorption and cellular uptake of FA were made on monogastric species in the last 20 years. We provide a model of FA utilization in dairy cows by using information generated in monogastrics and enriching it with data produced in dairy cows. We also reviewed the latest studies on the effects of dietary FA on milk yield, milk fatty acid composition, reproduction, and health in dairy cows. The reviewed data revealed a complex picture with the FA being active in each step of the way, starting from influencing rumen microbiota, regulating intestinal absorption, and affecting cellular uptake and utilization by peripheral tissues, making prediction on in vivo nutrigenomic effects of FA challenging.

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“…Growing evidence has revealed that miRNAs play a crucial function in the milk fat synthesis [8,[23][24][25]. miR-143 is one of the miRNAs closely related to lipid metabolism [14]; previous studies indicated that miR-143 participates in the differentiation of adipocytes, as well as triglyceride synthesis [15][16][17][18].…”

Section: Discussionmentioning

confidence: 99%

MiR-143 Regulates Milk Fat Synthesis by Targeting Smad3 in Bovine Mammary Epithelial Cells

Zhang

Wang

et al. 2020

Animals

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Milk fat is the main nutritional component of milk and is also an important indicator for evaluating milk quality. Substantial evidence has implicated miRNAs in the synthesis of milk fat. miR-143 is one of the miRNAs closely related to lipid metabolism. Herein, miR-143 upregulation remarkably promoted the production of lipid droplets and increased the level of intracellular triglyceride (TAG). Meanwhile, miR-143 suppression overtly repressed TAG synthesis and lipid droplet accumulation in bovine mammary epithelial cells (BMECs). At the same time, miR-143 significantly upregulated the genes associated with lipid synthesis, including PPARγ, SCD1, CEBPβ, and SREBP1. To examine the regulatory mechanism of miR-143 in milk fat synthesis, Smad3 was predicted as a new potential miR-143 target gene by TargetScan. Further studies found that miR-143 expression was inversely related to the levels of Smad3 mRNA and protein. Furthermore, luciferase reporter assays confirmed Smad3 to be a miR-143 direct target. Moreover, Smad3 gene silencing significantly increased intracellular TAG level in BMECs. These findings revealed that miR-143 promotes the TAG synthesis in BMECs via increasing the lipid synthesis related gens expression by targeting Smad3. The results of this study can be exploited in devising novel approaches for improving the nutritional value of milk in dairy cows.

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miR-454 regulates triglyceride synthesis in bovine mammary epithelial cells by targeting PPAR-γ

Cited by 21 publications

References 44 publications

MiRNA Profiling in Pectoral Muscle Throughout Pre- to Post-Natal Stages of Chicken Development

MiRNA Profiling in Pectoral Muscle Throughout Pre- to Post-Natal Stages of Chicken Development

Advances in fatty acids nutrition in dairy cows: from gut to cells and effects on performance

MiR-143 Regulates Milk Fat Synthesis by Targeting Smad3 in Bovine Mammary Epithelial Cells

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