miR-484 mediates oxidative stress-induced ovarian dysfunction and promotes granulosa cell apoptosis via SESN2 downregulation

Wang, Xiaofei; Yang, Jiahao; Li, Huiying; Mu, Hongbei; Cai, Siying; Su, Ping; Li, Huaibiao; Zhang, Ling; Xiang, Wenpei

doi:10.1016/j.redox.2023.102684

Cited by 26 publications

(7 citation statements)

References 44 publications

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“…Simultaneously, MT-CD treatments reduced MMP loss caused by H 2 O 2 (Figure E, H). The reduction in mitochondrial membrane potential is an important event in the initial phase of cell apoptosis . This also indirectly indicates that oxidative damage is intricately linked to the regulation of specific cellular functions including apoptosis.…”

Section: Results and Discussionmentioning

confidence: 99%

Melatonin-Derived Carbon Dots with Free Radical Scavenging Property for Effective Periodontitis Treatment via the Nrf2/HO-1 Pathway

Xin,

Liu,

Liu

et al. 2024

ACS Nano

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Periodontitis is a chronic inflammatory disease closely associated with reactive oxygen species (ROS) involvement. Eliminating ROS to control the periodontal microenvironment and alleviate the inflammatory response could potentially serve as an efficacious therapy for periodontitis. Melatonin (MT), renowned for its potent antioxidant and antiinflammatory characteristics, is frequently employed as an ROS scavenger in inflammatory diseases. However, the therapeutic efficacy of MT remains unsatisfactory due to the low water solubility and poor bioavailability. Carbon dots have emerged as a promising and innovative nanomaterial with facile synthesis, environmental friendliness, and low cost. In this study, melatoninderived carbon dots (MT-CDs) were successfully synthesized via the hydrothermal method. The MT-CDs have good water solubility and biocompatibility and feature excellent ROS-scavenging capacity without additional modification. The in vitro experiments proved that MT-CDs efficiently regulated intracellular ROS, which maintained mitochondrial homeostasis and suppressed the production of inflammatory mediators. Furthermore, findings from the mouse model of periodontitis indicated that MT-CDs significantly inhibited the deterioration of alveolar bone and reduced osteoclast activation and inflammation, thereby contributing to the regeneration of damaged tissue. In terms of the mechanism, MT-CDs may scavenge ROS, thereby preventing cellular damage and the production of inflammatory factors by regulating the nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) pathway. The findings will offer a vital understanding of the advancement of secure and effective ROSscavenging platforms for more biomedical applications.

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Section: Results and Discussionmentioning

confidence: 99%

Melatonin-Derived Carbon Dots with Free Radical Scavenging Property for Effective Periodontitis Treatment via the Nrf2/HO-1 Pathway

Xin,

Liu,

Liu

et al. 2024

ACS Nano

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“…Concerning the ovarian functions of major involvement, we found that hsa-miR-484, demonstrating a statistically significant up-regulation (mean fold change = 4.33, p -value = 0.0011) in our analysis, is known to repress the proliferation of granulosa cells (GCs) and promote apoptosis in GCs due to oxidative-stress-induced ovarian dysfunction [ 43 , 44 ]. Moreover, this miRNA was disclosed to directly target YAP1 mRNA, inducing mitochondrial dysfunction and promoting the apoptosis of GCs, as a possible mechanism underlying diminished ovarian reserve [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

“…In miRNA studies, miR-222 has been observed in different stages of follicle development [ 44 ]. In a recent investigation on rats and humans, miR-222 was found to be related to the etiology of PCOS [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

Maternal MicroRNA Profile Changes When LH Is Added to the Ovarian Stimulation Protocol: A Pilot Study

Konstantinidou,

Placidi,

Di Emidio

et al. 2023

Epigenomes

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While the use of follicle-stimulating hormone (FSH) in ovarian stimulation for in vitro fertilization (IVF) is an established practice, the use of luteinizing hormone (LH) remains debatable. MicroRNAs (miRNAs) are short, endogenous, non-coding transcripts that control a variety of cellular functions, such as gonadotrophin production and follicular development. The goal of this pilot study was to investigate whether the employment of recombinant LH (rLH) in ovarian stimulation protocols results in changes in the miRNA profiles in human oocytes. Patients were divided into two groups: seven received recombinant FSH (rFSH, 225 IU), and six received rFSH (150 IU) plus rLH (75 IU). MiRNA predesigned panels and real-time PCR technology were used to analyze the oocytes retrieved from the follicular ovarian retrieval. Among the miRNAs evaluated, a series of them evidenced upregulation or downregulation in their expression in the FSH plus LH group compared to the FSH group. Considering the results obtained from the functional and network analysis, the different maternal miRNA profiles in the two groups revealed a differential modulation of pathways involved in numerous biological functions. Overall, based on the pathways associated with most of these maternal miRNAs, the presence of LH may result in a different modulation of pathways regulating survival under the control of a Tp53-related mechanism. Interestingly, among the miRNAs differentially expressed in oocytes of the two groups, we have found miRNAs already investigated at ovarian, follicular, oocyte, and embryonic levels: hsa-miR-484, hsa-miR-222, hsa-miR-520d-5p, hsa-miRNA-17, hsa-miR-548, and hsa-miR-140. Thus, investigation into the role of these miRNAs in oocyte molecular pathways may help determine how LH affects oocyte competence and eventually leads to the clinical improvement of IVF.

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“…MiRNAseq revealed the top ten most enriched miRNAs: miR-125b-5p, miR-21-5p, miR-143-3p, let-7i-5p, miR-146a-5p, miR-125b-5p, miR-199b-3p, miR-16-5p, miR-221-3p and let-7c-5p (Figure .5A,B). We searched for miRNAs that were involved in oxidative stress (miR-181a-5p, miR-126, miR-146a-5p, miR-125b-5p, miR-484, and miR-27) [22][23][24][25][26][27]. MiR-125b-5p and miR-146a-5p were candidates that were abundant in MSC-sEVs and were likely associated with ferroptosis (Figure .…”

Section: Mir-125b-5p Enrichment In Msc-sevs Suppresses Ferroptosismentioning

confidence: 99%

Mesenchymal Stem Cell-derived Exosomal miR-125b-5p Suppressed Retinal Microvascular Endothelial Cell Ferroptosis by Targeting P53 in Diabetic Retinopathy

Tong,

Chen,

Xiang

et al. 2024

Preprint

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Progressive endothelial cell injury of retinal vascular is a vital factor in diabetic retinopathy (DR) pathogenesis. Mesenchymal stromal cells-derived small extracellular vesicles (MSC-sEVs) showed beneficial effects on DR. However, the effects of MSC-sEVs in endothelial dysfunction of DR and the mechanism is still unclear. In this study, MSC-sEVs mitigated retinal blood-retina barrier(BRB) impairment in rats with streptozotocin (STZ)-induced DR by reducing ferroptosis in vivo and in vitro. MSC-sEVs miRNA sequencing analysis revealed that miR-125b-5p may mediate HRMEC ferroptosis and P53 as a downstream target based on dual-luciferase reporter assays. Silencing miR-125b-5p in MSC-sEVs reversed the therapeutic effects of MSC-sEVs on rats with DR and advanced glycation end products (AGE)-treated HRMECs. Additionally, overexpression of miR-125b-5p could diminish ferroptosis in HRMECs, and this effect could be effectively reversed by overexpressing P53. This study indicated the potential therapeutic effect of MSC-sEVs on vascular endothelial function maintenance and that the delivery of sEVs carrying miR-125b-5p could prevent endothelial cell ferroptosis by inhibiting P53, thereby protecting the BRB.

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miR-484 mediates oxidative stress-induced ovarian dysfunction and promotes granulosa cell apoptosis via SESN2 downregulation

Cited by 26 publications

References 44 publications

Melatonin-Derived Carbon Dots with Free Radical Scavenging Property for Effective Periodontitis Treatment via the Nrf2/HO-1 Pathway

Melatonin-Derived Carbon Dots with Free Radical Scavenging Property for Effective Periodontitis Treatment via the Nrf2/HO-1 Pathway

Maternal MicroRNA Profile Changes When LH Is Added to the Ovarian Stimulation Protocol: A Pilot Study

Mesenchymal Stem Cell-derived Exosomal miR-125b-5p Suppressed Retinal Microvascular Endothelial Cell Ferroptosis by Targeting P53 in Diabetic Retinopathy

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