MiR-486-5p-directed MAGI1/Rap1/RASSF5 signaling pathway contributes to hydroquinone-induced inhibition of erythroid differentiation in K562 cells

Ma, Xiaoju; Zhang, Xue; Luo, Jiao; Liang, Beibei; Peng, Jing; Chen, Chuanying; Guo, Hongqian; Wang, Qing; Xing, Xiumei; Deng, Qifei; Huang, Hongyun; Liao, Qilong; Chen, Wen; Hu, Qiansheng; Yu, Dianke; Xiao, Yongmei

doi:10.1016/j.tiv.2020.104830

Cited by 16 publications

(10 citation statements)

References 56 publications

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“…Consistently, miR-27a-3p overexpression obviously reduced the expression of RASSF5, and miR-27a-3p inhibitor increased the expression of RASSF5 in NP cells. ere have been some studies shown that RASSF5 is regulated by microRNAs such as miR-532-5p [9], miR-214 [17], and miR-486-5p [18] and plays a cardinal role in cell proliferation and apoptosis. According to the paper of Zhu miR-532-5p regulated RASSF5 negatively to inhibit cell apoptosis and reduce alleviate IDD [9].…”

Section: Discussionmentioning

confidence: 99%

Role of MiR-27a-3p in Intervertebral Disc Degeneration through Targeting RASSF5 via MST1/LATS1 and RAS/RAC1 Signaling Pathway

Yuan

Zhou

et al. 2022

Journal of Healthcare Engineering

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Background. The apoptosis of nucleus pulposus (NP) cells reduces the number of nucleus pulposus cells in intervertebral disc tissue, resulting in intervertebral disc degeneration (IDD). MicroRNAs (miRNAs) play an important regulatory role in abnormal cell proliferation and apoptosis. Methods. The miR-27a-3p expressions in degenerative NP tissue and cells were measured via qPCR. The impacts of miR-27a-3p on the proliferation and apoptosis of human NP cells were evaluated by flow cytometry assays, MTT assays, and western blot analyses. In addition, target scan and luciferase reporter assay were applied to confirm that RASSF5 was directly binding to miR-27a-3p. Western blot was applied to assess the relationship between miR-27a-3p, RASSF5 and MST1/LATS1, and RAS/RAC1 signaling pathway. Results. MiR-27a-3p was downregulated in degenerative NP tissues and cells by comparison with the control group. MiR-27a-3p overexpression enhanced cell proliferation and suppressed apoptosis of NP cells, while the above factors showed an opposite tendency after in the miR-27a-3p inhibitor group. The western blot experiment similarly suggested mir-27a-3p apparently downregulated apoptosis-related proteins (Bax and caspase-3) and upregulated antiapoptotic proteins (Bcl-2). In addition, RASSF5 was confirmed to be directly regulated by miR-27a-3p using the luciferase reporter assay. Overexpressed RASSF5 could reverse the effects caused by miR-27a-3p mimic. Finally, miR-27a-3p could downregulate RASSF5 and affected the MST1/LATS1 and RAS/RAC1 pathway. Conclusion. MiR-27a-3p may target RASSF5 and enhance cell proliferation and imped cell apoptosis of the nucleus pulposus cells via the MST1/LATS1 and RAS/RAC1 pathway, lessening the degeneration of intervertebral discs.

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Section: Discussionmentioning

confidence: 99%

Role of MiR-27a-3p in Intervertebral Disc Degeneration through Targeting RASSF5 via MST1/LATS1 and RAS/RAC1 Signaling Pathway

Yuan

Zhou

et al. 2022

Journal of Healthcare Engineering

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“…Finally, ZN528, or ZNF protein 528, is implicated in gene expression regulation, cell proliferation and differentiation [ 47 ]. Its role in activating Rap1 signaling in K562 cells is crucial for their proliferation and survival [ 48 , 49 ]. ZN528 also regulates IL-1 receptor expression, central to inflammatory responses [ 50 ].…”

Section: Resultsmentioning

confidence: 99%

CEMIG: prediction of the cis-regulatory motif using the de Bruijn graph from ATAC-seq

Wang,

Li,

Wang

et al. 2023

Briefings in Bioinformatics

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Sequence motif discovery algorithms enhance the identification of novel deoxyribonucleic acid sequences with pivotal biological significance, especially transcription factor (TF)-binding motifs. The advent of assay for transposase-accessible chromatin using sequencing (ATAC-seq) has broadened the toolkit for motif characterization. Nonetheless, prevailing computational approaches have focused on delineating TF-binding footprints, with motif discovery receiving less attention. Herein, we present Cis rEgulatory Motif Influence using de Bruijn Graph (CEMIG), an algorithm leveraging de Bruijn and Hamming distance graph paradigms to predict and map motif sites. Assessment on 129 ATAC-seq datasets from the Cistrome Data Browser demonstrates CEMIG’s exceptional performance, surpassing three established methodologies on four evaluative metrics. CEMIG accurately identifies both cell-type-specific and common TF motifs within GM12878 and K562 cell lines, demonstrating its comparative genomic capabilities in the identification of evolutionary conservation and cell-type specificity. In-depth transcriptional and functional genomic studies have validated the functional relevance of CEMIG-identified motifs across various cell types. CEMIG is available at https://github.com/OSU-BMBL/CEMIG, developed in C++ to ensure cross-platform compatibility with Linux, macOS and Windows operating systems.

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“…Another level of MAGI dysregulation relates to the targeting of the corresponding transcripts by a series of microRNAs ( Table 2 ). MAGI1 mRNAs are down-regulated by miR-486-5p in the human erythroid leukemia K562 cell line, leading to decreased RA-GEF-1 /Rap1A signaling pathway and inhibition of erythroid differentiation [ 136 ]. In renal cell carcinoma, a low MAGI1 expression level, as a result of c-Myb-induced miR-520h transactivation, is associated with a poor prognosis [ 137 ].…”

Section: Implication Of Magi Molecular Scaffolds In the Control Of Carcinogenesismentioning

confidence: 99%

A New Story of the Three Magi: Scaffolding Proteins and lncRNA Suppressors of Cancer

Kotelevets

Chastre

2021

Cancers

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Scaffolding molecules exert a critical role in orchestrating cellular response through the spatiotemporal assembly of effector proteins as signalosomes. By increasing the efficiency and selectivity of intracellular signaling, these molecules can exert (anti/pro)oncogenic activities. As an archetype of scaffolding proteins with tumor suppressor property, the present review focuses on MAGI1, 2, and 3 (membrane-associated guanylate kinase inverted), a subgroup of the MAGUK protein family, that mediate networks involving receptors, junctional complexes, signaling molecules, and the cytoskeleton. MAGI1, 2, and 3 are comprised of 6 PDZ domains, 2 WW domains, and 1 GUK domain. These 9 protein binding modules allow selective interactions with a wide range of effectors, including the PTEN tumor suppressor, the β-catenin and YAP1 proto-oncogenes, and the regulation of the PI3K/AKT, the Wnt, and the Hippo signaling pathways. The frequent downmodulation of MAGIs in various human malignancies makes these scaffolding molecules and their ligands putative therapeutic targets. Interestingly, MAGI1 and MAGI2 genetic loci generate a series of long non-coding RNAs that act as a tumor promoter or suppressor in a tissue-dependent manner, by selectively sponging some miRNAs or by regulating epigenetic processes. Here, we discuss the different paths followed by the three MAGIs to control carcinogenesis.

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MiR-486-5p-directed MAGI1/Rap1/RASSF5 signaling pathway contributes to hydroquinone-induced inhibition of erythroid differentiation in K562 cells

Cited by 16 publications

References 56 publications

Role of MiR-27a-3p in Intervertebral Disc Degeneration through Targeting RASSF5 via MST1/LATS1 and RAS/RAC1 Signaling Pathway

Role of MiR-27a-3p in Intervertebral Disc Degeneration through Targeting RASSF5 via MST1/LATS1 and RAS/RAC1 Signaling Pathway

CEMIG: prediction of the cis-regulatory motif using the de Bruijn graph from ATAC-seq

A New Story of the Three Magi: Scaffolding Proteins and lncRNA Suppressors of Cancer

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