MiR‐490‐3p inhibits autophagy via targeting ATG7 in hepatocellular carcinoma

Abstract

The miR-490-3p was transfected into HepG2 cells to explore the correlation between miR-490-3p and hepatocellular carcinoma cell proliferation, apoptosis, and autophagy and its downstream target gene ATG7. Then we could possibly provide a mechanism for the treatment of hepatocellular carcinoma. MiR-490-3p was screened out by fold change > 4 and P < 0.01 using gene microarray data. The expression level of miR-490-3p was tested by qRT-PCR and the prognosis analysis was achieved by using TCGA data. The cell proliferation was tested via colony formation assay and CCK-8 after the miR-490-3p mimics were transfected into HepG2 cells; the variations of cell cycle and apoptosis was examined by flow cytometry assay; the number of autophagosome was observed by electron microscopy and the changes of autophagy-relative protein LC-II and LC-I as well as their ratio was tested by western blot. MiR-490-3p is low expressed in hepatocellular carcinoma cell lines and tissues. The results of TCGA showed that miR-490-3p high expression indicated better prognosis. After HepG2 cells were transfected with miR-490-3p mimics, cell viability was increased, cell proliferation was enhanced, cell cycle was blocked in G0/G1 phase, cell apoptosis rate was increased, the number of autophagosomes was reduced, autophagy-associated protein LC-II was decreased, and LC-I was increased and their ratio was decreased. After 3-MA was added, cell proliferation was declined, cell apoptosis rate was increased. Besides, the autophagy was inhibited by knocking down the ATG7, which promoted the cell apoptosis. MiR-490-3p could suppress cell proliferation, retard cell cycle and upgrade cell apoptosis by inhibiting autophagy in HCC cells via targeting ATG7. © 2018 IUBMB Life, 70(6):468-478, 2018.

miRNA‑490‑3p promotes the metastatic progression of invasive ductal carcinoma

“…miR-490 functions as a tumor suppressor in epithelial ovarian cancer, colorectal cancer, renal cell carcinoma and bladder cancer (8)(9)(10)(11)(12). Even within the context of hepatocellular and lung carcinoma, the association of miR-490-3p expression with disease progression seems to be context-dependent (13)(14)(15)(16).…”

“…Another interesting aspect is that different studies have identified different targets of miR-490-3p, including ERGIC3, CDK1, ATG7, VDAC1, PIK3CA, CCND1 and PCBP1 (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18). The present study solely focused on PCBP1, as it has been shown to be targeted by miR-490-3p (16) and due to its well-documented role as a tumor suppressor in breast cancer (23,24).…”

“…miR-490-3p and miR-490-5p has been shown to function as tumor suppressors in colorectal cancer, renal cell carcinoma, epithelial ovarian cancer and bladder cancer (9)(10)(11)(12)(13). However, separate studies have demonstrated that miR-490-3p can function as a tumor suppressor or promoter in lung and hepatocellular carcinoma (14)(15)(16)(17). miR-4903-3p has also been…”

<p>MiR-490-3p Inhibits the Malignant Progression of Lung Adenocarcinoma</p>

“…8 MiR-490-3p inhibits the proliferation, delays cell cycle and promotes apoptosis through inhibiting autophagy of hepatocellular carcinoma cells. 9 However, the role and mechanism of miR-490-3p in LUAD cells have not been reported.…”

lncRNA-SNHG15 accelerates the development of hepatocellular carcinoma by targeting miR-490-3p/ histone deacetylase 2 axis

“…Moreover, miR-490-3p sponging by CircSLC3A2 was reported to regulate HCC progression[12]. It had been shown that miR-490-3p expression was reduced in HCC and restrained autophagy[13]. However, whether SNHG15 regulates HCC progression via regulating miR-490-3p remains unknown.…”