miR-490-3p Modulates Cell Growth and Epithelial to Mesenchymal Transition of Hepatocellular Carcinoma Cells by Targeting Endoplasmic Reticulum-Golgi Intermediate Compartment Protein 3 (ERGIC3)

Zhang, Lingyun; Liu, Min; Li, Xin; Tang, Hua

doi:10.1074/jbc.m112.410506

Cited by 131 publications

(124 citation statements)

References 49 publications

Supporting

Mentioning

117

Contrasting

Unclassified

Order By: Relevance

“…Hart et al recently reported the possible role of miR-490-3p in ER trafficking machinery regulation [84]. Furthermore, a direct interaction between miR-490-3p and ER-Golgi intermediate compartment protein 3 (ERGIC3) has been demonstrated in HCC cells [85]. Since the function of ERGIC3 is unclear, further studies are needed to evaluate its role in ER trafficking, as well as the role of miR-490-3p in this pathway.…”

Section: Upr Mirnasmentioning

confidence: 99%

Regulation of the unfolded protein response by microRNAs

Bartoszewska¹,

Kochan²,

Madanecki³

et al. 2013

Cellular and Molecular Biology Letters

View full text Add to dashboard Cite

The unfolded protein response (UPR) is an adaptive response to the stress that is caused by an accumulation of misfolded proteins in the lumen of the endoplasmic reticulum (ER). It is an important component of cellular homeostasis. During ER stress, the UPR increases the protein-folding capacity of the endoplasmic reticulum to relieve the stress. Failure to recover leads to apoptosis. Specific cellular mechanisms are required for the cellular recovery phase after UPR activation. Using bioinformatics tools, we identified a number of microRNAs that are predicted to decrease the mRNA expression levels for a number of critical components of the UPR. In this review, we discuss the potential role of microRNAs as key regulators of this pathway and describe how microRNAs may play an essential role in turning off the UPR after the stress has subsided.

show abstract

Section: Upr Mirnasmentioning

confidence: 99%

Regulation of the unfolded protein response by microRNAs

Bartoszewska¹,

Kochan²,

Madanecki³

et al. 2013

Cellular and Molecular Biology Letters

View full text Add to dashboard Cite

show abstract

“…3C), demonstrating that miR-346 regulates the activity of other miRNAs by increasing AGO2 expression. To further validate the effect of miR-346 on the activity of other miRNAs, we investigated the influence of miR-346 on the activity of miR-490 -3p, which has been reported to enhance the expression of ERGIC3 in an AGO2-dependent manner (24). As shown in Fig.…”

Section: Grsf1 Participates In the Up-regulation Of Ago2 In A Mir-346mentioning

confidence: 99%

“…Western Blot Assay-The detailed procedure for Western blotting has been described elsewhere (24). The primary antibodies used in this study included GFP, PTEN, AGO2, ERGIC3, CDK2, CHL1, and GAPDH, which were obtained from Saier Biotechnology Co. (Tianjin, China).…”

Section: Cell Lines and Clinical Tissue Samples-mentioning

confidence: 99%

miR-346 Up-regulates Argonaute 2 (AGO2) Protein Expression to Augment the Activity of Other MicroRNAs (miRNAs) and Contributes to Cervical Cancer Cell Malignancy

Guo

Liu

et al. 2015

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are a class of post-transcriptional regulators of gene expression, and AGO2 is essential for miRNA activity. In this study, we focused on the regulation of AGO2 by miR-346 and the consequences in cervical cancer cells. miR-346 enhanced the expression of AGO2, resulting in the increased activity of other miRNAs and contributing to the malignancy of HeLa cells. GRSF1 participated in the regulation of AGO2 by miR-346, and the middle sequence of miR-346 was vital for the synergy effect of miR-346 and GRSF1. We determined that miR-346 promoted the migration and invasion of HeLa cells. In summary, we are the first to report that AGO2 is regulated positively by miRNA and that GRSF1 participates in the miRNA pathway.Cervical cancer is one of the most prevalent gynecological cancers worldwide, accounting for ϳ12% of all cancers globally (1). The etiology of cervical cancer varies, and high-risk human papilloma virus infection is one of the most prominent features of cervical cancer (2). Recently, the role of microRNAs (miRNAs), 2 a class of small RNAs that post-transcriptionally regulate target gene expression in a sequence-specific manner, has received widespread attention (3, 4). The dysregulation of miRNAs affects many aspects of cervical cancer, including cellular apoptosis, cell cycle progression, and cell migration and invasion (5-7). miRNAs are transcribed as long primary transcripts that are subsequently processed by the RNase III proteins Drosha and Dicer to form mature microRNAs (8). Then, a strand of mature miRNA is incorporated into an RNA-induced silencing complex (RISC), where it exerts its effects. AGO2 is a key component of the RISC and is the only AGO2 protein with intrinsic endonuclease activity (9). The expression of AGO2 is tightly regulated at both transcriptional and post-transcriptional levels (10). Dysregulation of AGO2 has been reported in many tumors, including lung cancer (11), prostate cancer (12, 13), hepatocarcinoma (14), and melanoma (15), but the role of AGO2 and how its expression is regulated in cervical cancer have not been well studied.In this study, we found that AGO2 was expressed in cervical cancers and that its expression was enhanced by miR-346 and GRSF1 independent of an increase in AGO2 stability. The increased expression of AGO2 augmented the activity of other miRNAs and promoted the malignant phenotype of cervical cancer cells. These findings could provide new insights into the regulation of AGO2 expression and the mechanisms by which miRNAs up-regulate the expression of their target genes. Materials and Methods Cell Lines and Clinical Tissue Samples-The cancer cell lines used in this study were preserved in liquid nitrogen in our laboratory, and the cells were maintained in standard culture medium as recommended by the ATCC. All transfections were performed with Lipofectamine TM 2000 reagent (Invitrogen) according to the recommendations of the manufacturer. Fourteen pairs of clinical cervical cancer tissues and respective adjacent non-cancerous tissues were...

show abstract

“…Reports have shown that miR-490-3p functions as a tumor suppressor in A549 lung cancer cells, gastric cancer and ovarian carcinoma by targeting CCND1, SMARCD1 and CDK1 [9][10][11]. miR-490-3p plays an oncogenic role in hepatocellular carcinoma cells by targeting endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3) [12]. Thus, whether miR-490-3p acts as a tumor suppressor or an oncogene may depend on the cellular context.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‐490‐3p regulates cell proliferation and apoptosis by targeting HMGA2 in osteosarcoma

Liu

et al. 2015

FEBS Letters

View full text Add to dashboard Cite

Edited by Tamas Dalmay Keywords:MicroRNA-490-3p High mobility group a isoform 2 Osteosarcoma Proliferation a b s t r a c t ) has been implicated in several human malignancies; however, its potential functions and the underlying molecular mechanisms in osteosarcoma progression remain largely unclear. Here, we showed that miR-490-3p was down-regulated in osteosarcoma cell lines. Ectopic expression of miR-490-3p decreased cell proliferation, induced G1 arrest and apoptosis in vitro and inhibited tumorigenicity in a mouse xenograft model. Furthermore, miR-490-3p bound directly to HMGA2 mRNA 3 0 UTR and mediated a decrease in HMGA2 mRNA and protein expression. Re-expression of HMGA2 reversed the inhibitory effects of miR-490-3p. Further investigations showed an inverse correlation between low miR-490-3p and high HMGA2 expression in osteosarcoma tissues. Taken together, our results suggest that miR-490-3p functions as a potential tumor suppressor by down-regulating HMGA2 expression directly, and it may represent a potential therapeutic target for patients with osteosarcoma.

show abstract

miR-490-3p Modulates Cell Growth and Epithelial to Mesenchymal Transition of Hepatocellular Carcinoma Cells by Targeting Endoplasmic Reticulum-Golgi Intermediate Compartment Protein 3 (ERGIC3)

Cited by 131 publications

References 49 publications

Regulation of the unfolded protein response by microRNAs

Regulation of the unfolded protein response by microRNAs

miR-346 Up-regulates Argonaute 2 (AGO2) Protein Expression to Augment the Activity of Other MicroRNAs (miRNAs) and Contributes to Cervical Cancer Cell Malignancy

MicroRNA‐490‐3p regulates cell proliferation and apoptosis by targeting HMGA2 in osteosarcoma

Contact Info

Product

Resources

About