miR-491-3p is Downregulated in Retinoblastoma and Inhibit Tumor Cells Growth and Metastasis by Targeting SNN

Hu, Yang; Zhao, Ming; Li, Li; Ding, Jie; Gui, Yu-Min; Wei, Tan-Wei

doi:10.1007/s10528-020-10007-w

Cited by 10 publications

(9 citation statements)

References 46 publications

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“…Furthermore, miR-1207-5p as one of the ten miRNAs expressed significantly different among gastric cancer patients with or without LNM via miRNA microarray, which could serve as a useful biomarker in the prediction of LNM [ 13 ]. MiR-491-3p, a tumor suppressor miRNA, was found decreased in many solid tissues, including brain tumor [ 14 ], colorectal cancer [ 15 ], and retinoblastoma [ 16 ]. It was worth mentioning that miR-491-3p was downregulated in gastric cancer [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Low Expression of miR-491-3p Is Correlated with Lymph Node Metastasis in Gastric Cancer

Luo

2022

Evidence-Based Complementary and Alternative Medicine

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Objective. MiR-491-3p, as a tumor suppressor miRNA, was found decreased in many solid tissues. In this study, we aim to investigate miR-491-3p expression in gastric cancer with or without lymph node metastasis (LNM). Methods. GSE173215 dataset from Gene Expression Omnibus (GEO) was used to measure miRNA expression from tissue samples of gastric cancer patients. Moreover, gastric tumor tissues (non-LNM: n = 78; LNM: n = 68) were obtained to detect the miR-491-3p expression. Receiver operating characteristic (ROC) curve and Kaplan–Meier (KM) survival analysis, as well as Cox regression analysis, were performed to reveal the role of miR-491-3p in diagnosis and prognosis of gastric cancer. Results. According to GSE173215 datasets (t = −11.25, adjust P value = 1.30E-06) and our clinical results (0.390 ± 0.193 vs. 0.562 ± 0.166, P < 0.005 ), the gastric cancer patients with LNM showed lower miR-491-3p expression than those without LNM, demonstrating a high diagnostic efficiency (sensitivity: 74.36%; specificity: 69.12%). In addition, both LNM and low miR-491-3p expression were correlated with the poor prognosis of gastric cancer. Furthermore, the LNM patient with low expression of miR-491-3p had the worse prognosis, but the non-LNM patient with high expression of miR-491-3p had the best prognosis. MiR-491-3p expression (HR = 0.003, 95%CI: 3.35E-04∼0.028) and LNM (HR = 2.326, 95%CI: 1.046∼5.173) were independent risk factors for gastric cancer. Conclusion. Downregulated miR-491-3p expression was found in gastric cancer, being a high diagnostic efficiency and an independent risk factor for gastric cancer, especially in those having LNM.

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Section: Introductionmentioning

confidence: 99%

Low Expression of miR-491-3p Is Correlated with Lymph Node Metastasis in Gastric Cancer

Luo

2022

Evidence-Based Complementary and Alternative Medicine

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“…HOTAIR propels carcinogenesis and shows an association with tumor initiation and progression, drug resistance, and poor survival rates of patients [ 28 ]. In previous studies concerning RB, most researchers have selected ARPE-19 cells as the control group [ 29 – 31 ]. The results of our study found no significant difference in HOTAIR expression between the two normal retinal cell lines ARPE-19 and RPE-1, and thus ARPE-19 cells could be utilized as the ideal control in our experiments.…”

Section: Discussionmentioning

confidence: 99%

LncRNA HOTAIR facilitates proliferation and represses apoptosis of retinoblastoma cells through the miR-20b-5p/RRM2/PI3K/AKT axis

Zhang

2022

Orphanet J Rare Dis

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Purpose Retinoblastoma (RB) represents an adolescent eye malignancy. Long non-coding RNA (LncRNA) HOTAIR shows aberrant expression in many malignancies. This research investigated the mechanism of HOTAIR in RB. Methods Normal retinal cell lines (ARPE-19 and RPE-1) and RB cell lines (ORB50, Y79, HXO-RB44, and WERI-RB) were selected for detection of HOTAIR expression by qRT-PCR. sh-HOTAIR was delivered into Y79 and HXO-RB44 cells. Cell-cycle distribution, proliferation, and apoptosis were detected by CCK-8 assay and flow cytometry. Binding relationships among HOTAIR, miR-20b-5p, and RRM2 were confirmed using dual-luciferase assay. Roles of miR-20b-5p and RRM2 in RB cell-cycle distribution, proliferation, and apoptosis were ascertained by functional rescue experiments. Murine model of xenograft tumor was established, followed by detection of tumor growth and counting of Ki67-positive cells. Expressions of proliferation- and apoptosis-associated proteins and PI3K/AKT pathway-related proteins were determined by Western blot. Results HOTAIR was elevated in RB cells relative to that in normal retinal cells and showed relatively high expression in Y79 and HXO-RB44 cells. sh-HOTAIR induced RB cell-cycle arrest, restrained proliferation, and strengthened apoptosis. HOTAIR functioned as the ceRNA of miR-20b-5p and targeted RRM2. RB cells had poorly-expressed miR-20b-5p and highly-expressed RRM2. miR-20b-5p downregulation or RRM2 overexpression facilitated RB cell-cycle and proliferation, suppressed apoptosis, and reversed the protective effect of sh-HOTAIR on RB. sh-HOTAIR reduced tumor growth and Ki67-positive cells in vivo and inactivated PI3K/AKT pathway. Conclusion LncRNA HOTAIR upregulated RRM2 by competitively binding to miR-20b-5p and activated PI3K/AKT pathway, thereby facilitating proliferation and repressing apoptosis of RB cells.

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“…MiR-491-3p exerts a tumour suppressor effect on many human malignancies, such as retinoblastoma ( Hu et al, 2021 ), osteosarcoma ( Duan et al, 2017 ), hepatocellular carcinoma (HCC) ( Zhao et al, 2017 ), and glioblastoma ( Li et al, 2015 ). MiR-491-3p expression is also decreased in CRC tissues and oxaliplatin-resistant CRC cells ( Guo et al, 2017 ; Tang et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

“…MiR-491-3p is one of the mature products of miR-491. Recently, miR-491-3p has been reported to be involved in the progression of several cancers, such as retinoblastoma, tongue cancer, glioblastoma, and gastric cancer ( Hu et al, 2021 ; Zheng et al, 2015 ; Li et al, 2015 ; Yu & Luo, 2022 ). Furthermore, it has been demonstrated that miR-491 facilitates CRC cell apoptosis by reducing Bcl-XL expression ( Nakano et al., 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Downregulated miRNA-491-3p accelerates colorectal cancer growth by increasing uMtCK expression

Tang

Lin

et al. 2022

PeerJ

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Colorectal carcinoma (CRC) is the second most frequent cancer worldwide. MiR-491-3p, a tumor-suppressive microRNA (miRNA, miR), has been revealed to be abnormally expressed in CRC tissues. Meanwhile, up-regulated ubiquitous mitochondrial creatine kinase (uMtCK) contributes to CRC cell proliferation. Here we aim to explore whether aberrant miR-491-3p expression promotes CRC progression through regulating uMtCK. To this end, miR-491-3p and uMtCK levels were assessed in CRC tissues using quantitative real-time PCR (qRT-PCR). The biological roles of miR-491-3p and uMtCK in regulating CRC growth were evaluated using colony formation assay and mouse Xenograft tumour model. We found that miR-491-3p expression was decreased in CRC tissues compared with matched para-cancerous tissues, whereas uMtCK expression was increased. Functionally, miR-491-3p overexpression repressed SW480 cell growth, whereas miR-491-3p depletion accelerated SW620 cell proliferation and growth. Inversely, uMtCK positively regulated CRC cell proliferation. Mechanistically, miR-491-3p post-transcriptionally downregulated uMtCK expression by binding to 3’-UTR of uMtCK. Consequently, restoring uMtCK expression markedly eliminated the role of miR-491-3p in suppressing CRC growth. Collectively, miR-491-3p functions as a tumour suppressor gene by repressing uMtCK, and may be a potential target for CRC treatment.

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miR-491-3p is Downregulated in Retinoblastoma and Inhibit Tumor Cells Growth and Metastasis by Targeting SNN

Cited by 10 publications

References 46 publications

Low Expression of miR-491-3p Is Correlated with Lymph Node Metastasis in Gastric Cancer

Low Expression of miR-491-3p Is Correlated with Lymph Node Metastasis in Gastric Cancer

LncRNA HOTAIR facilitates proliferation and represses apoptosis of retinoblastoma cells through the miR-20b-5p/RRM2/PI3K/AKT axis

Downregulated miRNA-491-3p accelerates colorectal cancer growth by increasing uMtCK expression

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