MiR-495 inhibits esophageal squamous cell carcinoma progression by targeting Akt1

Mao, Yu; Liang, Li; Liu, Jia; Wang, Le; Zhou, Yan

doi:10.18632/oncotarget.9981

Cited by 44 publications

(38 citation statements)

References 30 publications

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“…Partly in line with our study, Dou et al have also clarified that upregulated PKIB can activate the PI3K/AKT signaling pathway in non‐small cell lung cancer . Although a recent study reported that miR‐495 could target AKT in 293 cells by dual‐luciferase report assay, our study only confirmed that miR‐495 might affect the expression of p‐AKT by targeting PKIB, but did not affect total AKT, thus inhibiting the PI3K/AKT signaling pathway. Similar results were found in a previous study that miR‐495 directly targets IGF1, and regulates AKT pathway activity in oral tumors through miR‐495/IGF1 axis .…”

Section: Discussionsupporting

confidence: 90%

microRNA‐495 reduces visceral sensitivity in mice with diarrhea‐predominant irritable bowel syndrome through suppression of the PI3K/AKT signaling pathway via PKIB

Fei

Wang

2020

IUBMB Life

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Diarrhea‐predominant irritable bowel syndrome (IBS‐D) is one of the most common gastrointestinal disorders in the world, lacking effective therapies. The crucial roles of microRNAs (miRNAs) in IBS‐D have attracted increasing attention. The aim of this study is to investigate the effects of miR‐495 on the visceral sensitivity of the IBS‐D through the PI3K/AKT signaling pathway by targeting PKIB. Microarray data analysis was employed to screen the differentially expressed genes related to IBS‐D and regulatory miRNAs. Then, mice were perfused with acetic acid into the rectum to establish the IBS‐D model. Next, PKIB expression was measured in IBS‐D mice. Additionally, model mice were injected with a series of adenovirus vector to investigate the influence of miR‐495 on visceral sensitivity and rectal function in IBS‐D mice with the involvement of PKIB and PI3K/AKT signaling pathway. The IBS‐D mouse model was successfully established. PKIB was the target gene of miR‐495, and highly expressed in mice with IBS‐D. Silencing PKIB reduced visceral sensitivity in mice with IBS‐D, and overexpression of miR‐495 decreased visceral sensitivity in mice with IBS‐D by inhibiting PKIB. Moreover, miR‐495 upregulation inhibited PI3K/AKT signaling pathway through downregulating PKIB. To sum up, this study reveals that miR‐495 upregulation can reduce visceral sensitivity in IBS‐D via inhibition of PI3K/AKT signaling pathway by targeting PKIB. It suggests that miR‐495 presents a potential target for IBS‐D therapy.

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Section: Discussionsupporting

confidence: 90%

microRNA‐495 reduces visceral sensitivity in mice with diarrhea‐predominant irritable bowel syndrome through suppression of the PI3K/AKT signaling pathway via PKIB

Fei

Wang

2020

IUBMB Life

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“…However, there are different views on the role of miR-495 in regulating tumor invasion and migration. It has been reported that miR-495 suppresses the aggressive behaviors of glioma [11], prostate cancer [12] and esophageal squamous cell carcinoma cells [13]. However, in breast [14], bladder [15] and gastric [16] cancer cells, this miRNA promotes tumor invasion and migration.…”

Section: Introductionmentioning

confidence: 98%

MicroRNA-495 suppresses osteosarcoma invasion and migration by targeting HSP90AA1

Xiao¹,

Wang²,

Li³

et al. 2018

Oncotarget

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MiR-495 is a tumor-suppressive microRNA that participates in tumor progression in human cancers. However, its expression and biological function in osteosarcoma remains unknown. In this study, we firstly found that miR-495 is markedly downregulated in both osteosarcoma tissues and cell lines. Then we demonstrated that miR-495 suppresses the invasion and metastasis of osteosarcoma cells in vitro through inhibiting epithelial to mesenchymal transition. Function of miR-495 in vivo was also examined in mice xenograft model and we found it significantly inhibiting the lung-metastasis of osteosarcoma cells. We also demonstrated that HSP90AA1 is a direct target of miR-495 using luciferase reporter assay and Western blot analysis. Furthermore, knockdown of HSP90AA1 can restore the increased cell invasion and migration in miR-495-knockdown cells and over expression of HSP90AA1 can neutralize the impaired metastatic ability of miR-495 mimic-treated cells. This metastasis-suppressive role of miR-495 in osteosarcoma is achieved by HSP90AA1-mediated PI3K/Akt/mTOR signaling pathway. Overall, our findings proved for the first time that miR-495 acts as a tumor suppressor in osteosarcoma and inhibits cell migration and invasion by targeting HSP90AA1, thus offering a promising therapeutic target for osteosarcoma treatment.www.impactjournals.com/oncotarget/

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“…Downregulation of miR-495 is frequently observed in various types of human cancer (21)(22)(23)(24). For example, miR-495 is downregulated in medulloblastoma tissues and cell lines; patients with medulloblastoma and decreased expression levels of miR-495 exhibit poorer prognosis compared with those expressing higher miR-495 levels (21).…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, miR-495 has been reported as an independent predictor of overall survival in patients with medulloblastoma (21). In esophageal squamous cell carcinoma, the expression levels of miR-495 were reported to be reduced in tumor tissues and significantly associated with lymph node metastasis, invasion and tumor, node and metastasis (TNM) stage (22). miR-495 was observed to be expressed at low levels in glioma (23,24), osteosarcoma (25), colorectal (35,36), gastric (37-39), prostate (40) and endometrial cancers (41), and renal cell carcinoma (42).…”

Section: Discussionmentioning

confidence: 99%

“…miR-495 was previously demonstrated to be dysregulated in human cancers, including medulloblastoma (21), esophageal squamous cell carcinoma (22), glioma (23,24) and osteosarcoma (25); however, the expression, functional roles and underlying molecular mechanism by which miR-495 regulates the progression of OSCC remain unclear. The present study aimed to detect the expression of miR-495 in OSCC tissues and cell lines, and determine its effects on OSCC cells.…”

Section: Introductionmentioning

confidence: 99%

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MicroRNA‑495 targets Notch1 to prohibit cell proliferation and invasion in oral squamous cell carcinoma

Wang

Yang

et al. 2018

Mol Med Report

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MicroRNAs (miRNAs) are associated with the initiation and progression of oral squamous cell carcinoma (OSCC) by regulating a variety of cancer-associated behaviors. Fully understanding the regulatory mechanism of miRNAs in the pathogenesis of OSCC may provide novel promising approaches for the identification of prognostic biomarkers and therapeutic targets for this particular malignancy. In the present study, reverse transcription-quantitative polymerase chain reaction analysis was performed to detect miRNA (miR)-495 expression in OSCC tissues and cell lines. The effects of miR-495 on the proliferation and invasion of OSCC cells were determined using Cell Counting Kit-8 and Matrigel invasion assays, respectively. The mechanisms underlying the action of miR-495 in OSCC cells were also investigated. Results from the present study revealed that miR-495 expression was downregulated in OSCC tissues and cell lines compare with in adjacent normal tissues and human oral keratinocytes, respectively. Exogenous expression of miR-495 restricted cell proliferation and invasion of OSCC cells in vitro. Notch1 was identified as a direct functional target of miR-495 in OSCC. Furthermore, Notch1 knockdown exhibited inhibitory effects, similar to those induced by miR-495 overexpression in OSCC cells. Restoration of Notch1 expression rescued the suppressive effects of miR-495 on OSCC cell proliferation and invasion. These findings suggested an important role for miR-495 in the regulation of OSCC cell growth and metastasis, at least partly by directly targeting Notch1. In addition, the findings of the present study revealed the potential of miR-495 as a novel therapeutic target for the treatment of patients with OSCC.

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MiR-495 inhibits esophageal squamous cell carcinoma progression by targeting Akt1

Cited by 44 publications

References 30 publications

microRNA‐495 reduces visceral sensitivity in mice with diarrhea‐predominant irritable bowel syndrome through suppression of the PI3K/AKT signaling pathway via PKIB

microRNA‐495 reduces visceral sensitivity in mice with diarrhea‐predominant irritable bowel syndrome through suppression of the PI3K/AKT signaling pathway via PKIB

MicroRNA-495 suppresses osteosarcoma invasion and migration by targeting HSP90AA1

MicroRNA‑495 targets Notch1 to prohibit cell proliferation and invasion in oral squamous cell carcinoma

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