miR-506 regulates breast cancer cell metastasis by targeting IQGAP1

Sun, Guang; Liu, Yanxi; Wang, Ke-Ren; Xu, Zhenhe

doi:10.3892/ijo.2015.3161

Cited by 32 publications

(21 citation statements)

References 28 publications

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“…On the other hand, it has been shown that the downregulation of hsa-miR-513c promotes the proliferation and invasiveness of neuroblastoma and glioma cells [38,39]. Similar to that downregulation of hsa-miR-506, leads to increased proliferation, invasiveness, and epithelial to mesenchymal transition in breast gastric and ovarian cancer [40][41][42]. Moreover, in vitro studies showed that overexpression of this miRNA is responsible for the increased sensitivity of colorectal cancer cells to chemotherapy [42].…”

Section: Discussionmentioning

confidence: 86%

The Potential Role of Selected miRNA in Uveal Melanoma Primary Tumors as Early Biomarkers of Disease Progression

Wróblewska

Lach

Ustaszewski

et al. 2020

Genes

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Uveal melanoma (UM) is the most common primary tumor of the eye diagnosed in adults, associated with a high risk of metastasis and thereby, poor prognosis. Among known risk factors for the development of metastatic disease is the loss of BAP1 expression and chromosome 3 monosomy in the primary tumor. However, the expression levels of specific micro RNAs (miRNA) in tumor tissue may also serve as a valuable marker for determining the risk of metastatic disease in patients with primary uveal melanoma. In our study, we analyzed the miRNA expression data of cases selected from The Cancer Genome Atlas study on uveal melanoma, and determined a panel of 15 miRNAs differentially expressed between patients with primary and metastatic disease. Next, 6 miRNAs were validated on a group of 46 tumor samples from primary and metastatic patients. We have shown, that expression of hsa-miR-592, hsa-miR-346, and hsa-miR-1247 was significantly increased, while hsa-miR-506 and hsa-miR-513c were decreased in the tumors of patients with metastatic disease. Hsa-miR-196b expression did not differ between the two subgroups, however, we showed significant correlation with BAP1 expression. Moreover, hsa-miR-592 also showed correlation with monosomy 3 tumors. Gene ontology analysis revealed involvement of those miRNAs with cellular processes mediating the metastatic process. Our results showed that miRNAs play an important role in the deregulation of several oncogenic pathways in UM and can, thereby, promote metastatic spread to distant organs. Moreover, differentially expressed miRNAs may be used as an interesting biomarker for the assessment of metastatic risk in uveal melanoma patients.

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Section: Discussionmentioning

confidence: 86%

The Potential Role of Selected miRNA in Uveal Melanoma Primary Tumors as Early Biomarkers of Disease Progression

Wróblewska

Lach

Ustaszewski

et al. 2020

Genes

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“…21,22 Furthermore, miR-506 inhibits cell proliferation, adhesion, and invasion in breast cancer cells by inactivating IQGAP1 and its downstream ERK/MAPK signaling pathways. 23 Ectopic expression of miR-506 suppressed epithelial-to-mesenchymal transition and angiogenesis in gastric cancer. 24 Depletion of miR-506 promoted apoptosis in retinoblastoma cell lines.…”

Section: Discussionmentioning

confidence: 99%

<p>LINC00963 Confers Oncogenic Properties in Glioma by Regulating the miR-506/BCAT1 Axis</p>

Ye¹,

Xu²,

Ge³

et al. 2020

CMAR

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Background: Glioma is a prevalent disease of the central nervous system with a high incidence and mortality rate. Many long noncoding RNAs (lncRNAs) have been determined to be critical regulators of glioma oncogenesis. However, the function and mechanism of LINC00963 in glioma have not been fully elucidated. Methods: The expression level of RNA was determined by qRT-PCR, and the protein level was determined by Western blot analysis. A luciferase activity assay was conducted to verify the interaction between miRNA and lncRNA or the target gene. The proliferation, cell cycle distribution, invasion, and migration were evaluated by MTT, EdU, flow cytometry, woundhealing and Transwell invasion assays, respectively. In vivo tumor growth was evaluated in a xenograft nude mouse model. Results: We found that LINC00963 was upregulated in glioma cells and tissues and associated with the poor prognosis of patients with glioma. Ectopic expression of LINC00963 promoted cell proliferation, cell cycle progression, migration, and invasion in vitro and tumorigenesis in vivo. Mechanistically, the results of luciferase activity and RNA pulldown assays validated that LINC00963 could act as a molecular sponge of miR-506. Reciprocal repression was found between LINC00963 and miR-506. In addition, BCAT1 was identified as a target of miR-506, and both the mRNA and protein levels of BCAT1 were reduced by miR-506. In tumor tissues, the expression of BCAT1 was negatively and positively correlated with miR-506 and LINC00963 expression, respectively. The reintroduction of BCAT1 in glioma cells abolished the tumor suppressive function of miR-506 by promoting cell viability and motility. The upregulated LINC00963 and BCAT1 were associated with the aggressive phenotypes of tumors. Conclusion: Our data revealed that LINC00963 confers oncogenic function in the progression of glioma and that the LINC00963/miR-506/BCAT1 axis may be a novel mechanism and therapeutic strategy for this disease.

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“…The overexpression of miR-506 inhibited the proliferation, metastasis, and EMT of breast cancer cells (35,36). Furthermore, miR-506 suppressed the metastasis of breast cancer cells by targeting the IQ motif containing GTPase-activating protein 1 (29). In gastric cancer, miR-506 inhibited EMT by targeting ETS1 (37) or SNAI2 (38).…”

Section: Discussionmentioning

confidence: 99%

“…Bioinformatic research revealed that FOXQ1 has a putative binding site for miR-506 (Fig. 4A), a well-recognized tumor-suppressor miRNA (28,29). A dual-luciferase reporter assay was performed to verify whether or not FOXQ1 is a target gene of miR-506.…”

Section: Foxq1 Is a Potential Target Of Mir-506mentioning

confidence: 99%

Suppression of forkhead box Q1 by microRNA-506 represses the proliferation and epithelial-mesenchymal transition of cervical cancer cells

Zhang

Yan

et al. 2016

Oncology Reports

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Abstract. MicroRNAs (miRNAs) play a pivotal role in cancer progression and development, representing novel therapeutic tools for cancer therapy. Forkhead box Q1 (FOXQ1) functions as an oncogene in various cancer types. However, the functional significance of FOXQ1 in cervical cancer remains unknown. In this study, we investigated the biological function of FOXQ1 in cervical cancer and tested whether or not FOXQ1 can be targeted and regulated by specific miRNAs. We found that FOXQ1 was highly expressed in cervical cancer cell lines. Knockdown of FOXQ1 by small interfering RNA (siRNA) significantly suppressed the proliferation and epithelial-mesenchymal transition (EMT) of cervical cancer cells. FOXQ1 was predicted as a target gene of microRNA-506 (miR-506), and this prediction was validated by dual-luciferase reporter assay. Quantitative real-time PCR and western blot analyses demonstrated that mRNA and protein expression was negatively regulated by miR-506. The expression of miR-506 was downregulated in cervical cancer tissues, and miR-506 expression was inversely correlated with FOXQ1 expression in cervical cancer. The overexpression of miR-506 dramatically suppressed the proliferation and EMT of cervical cancer cells that mimicked the suppression of FOXO1 siRNA. Furthermore, the restoration of FOXQ1 expression significantly reversed the inhibitory effect of miR-506. Overall, our study demonstrated that miR-506 inhibited the proliferation and EMT of cervical cancer cells by targeting FOXQ1 and provided evidence that the miR-506/FOXQ1 axis plays an important role in the pathogenesis of cervical cancer, representing potential molecular targets for the development of anticancer agents for cervical cancer treatment.

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miR-506 regulates breast cancer cell metastasis by targeting IQGAP1

Cited by 32 publications

References 28 publications

The Potential Role of Selected miRNA in Uveal Melanoma Primary Tumors as Early Biomarkers of Disease Progression

The Potential Role of Selected miRNA in Uveal Melanoma Primary Tumors as Early Biomarkers of Disease Progression

<p>LINC00963 Confers Oncogenic Properties in Glioma by Regulating the miR-506/BCAT1 Axis</p>

Suppression of forkhead box Q1 by microRNA-506 represses the proliferation and epithelial-mesenchymal transition of cervical cancer cells

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