miR-526b-5p/c-Myc/Foxp1 participates in recurrent spontaneous abortion by regulating the proliferation, migration, and invasion of trophoblasts

Li, Luo; Lu, Yao; Xie, Youlong; Chen, Enxiang; Ding, Yubin; Ge, Luxing

doi:10.1007/s10815-023-02793-0

Cited by 5 publications

(2 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For the purpose of early detection and treatment, it is crucial to identify possible biomarkers and therapeutic targets linked to RSA. Several studies show that the defects of trophoblast cells may cause RSA (Chen et al., 2023 ; Luo et al., 2023 ; Ma et al., 2023 ). Therefore, trophoblast cell function is closely associated with RSA occurrence and development.…”

Section: Introductionmentioning

confidence: 99%

Role of increased IGFBP2 in trophoblast cell proliferation and recurrent spontaneous abortion development: A pilot study

Ji,

Jiao,

Zhang

et al. 2024

Physiological Reports

View full text Add to dashboard Cite

Recurrent spontaneous abortion (RSA) is a serious condition that adversely affects women's health. Differentially expressed proteins (DEPs) in plasma of patients experiencing RSA is helpful to find new therapeutic targets and identified with mass spectrometry. In 57 DEPs, 21 were upregulated and 36 were downregulated in RSA. Gene ontology analyses indicated that identified DEPs were associated with cell proliferation, including significantly downregulated insulin‐like growth factor binding protein 2 (IGFBP2). Immunohistochemical result using clinical decidual tissues also showed that IGFBP2 expression was significantly decreased in RSA trophoblasts. Cell proliferation assay indicated that IGFBP2 treatment increased the proliferation and mRNA expressions of PCNA and Ki67 in trophoblast cells. Transcriptome sequencing experiments and Kyoto Encyclopedia of Genes and Genomes analyses revealed that gene expression for components in PI3K‐Akt pathway in trophoblasts was significantly upregulated following IGFBP2 treatment. To confirm bioinformatics findings, we did cell‐based experiments and found that treatment of inhibitors for insulin‐like growth factor (IGF)‐1 receptor‐PI3K‐Akt pathway significantly reduced IGFBP2‐induced trophoblast cell proliferation and mRNA expressions of PCNA and Ki67. Our findings suggest that IGFBP2 may increase trophoblast proliferation through the PI3K‐Akt signaling pathway to affect pregnancy outcomes and that IGFBP2 may be a new target for future research and treatment of RSA.

show abstract

Section: Introductionmentioning

confidence: 99%

Role of increased IGFBP2 in trophoblast cell proliferation and recurrent spontaneous abortion development: A pilot study

Ji,

Jiao,

Zhang

et al. 2024

Physiological Reports

View full text Add to dashboard Cite

show abstract

“…Qi Zhu found that miR-98 was highly expressed and could induce SA targeting GDF6 and FAPP2 to prevent proliferation, viability and migration of trophoblast cells in the villous tissues of SA, and Zhang CY also reported that MicroRNA-940 was up-regulated and promoted the occurrence of SA via targeting ZNF672 to inhibit the proliferation of trophoblast cells [ 10 , 11 ]. Some studies have further reported the role of miRNA in RSA, for example: up-regulation of miR-365 may contribute to recurrent miscarriage by decreasing SGK1 expression [ 1 ]; MiR-184 is up-regulated in the villus and decidua of RSA patients [ 12 ]; high expression of miR-526b-5p could cause RSA via targeting c-Myc and Foxp1 to promote the proliferation, migration, and invasion of trophoblasts [ 13 ]. However, because there are few studies on miRNA in URSA, the potential mechanisms of miRNA regulating URSA progression remain unclear.…”

Section: Introductionmentioning

confidence: 99%

MiR-150-5p contributes to unexplained recurrent spontaneous abortion by targeting VEGFA and downregulating the PI3K/AKT/mTOR signaling pathway

Liao,

Deng,

Chen

et al. 2023

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose The purpose of this study is to investigate the function of miR-150-5p in URSA. Method Twenty-six chorionic villous tissues were collected to examine the expression of miR-150-5p and VEGFA by using quantitative polymerase chain reaction (qPCR) and western blot assay, respectively. Transwell assay was conducted to assess the migration and invasion ability of trophoblast cells. The dual-luciferase reporter assay was applied to determine the relationship between miR-150-5p and VEGFA in vitro. Relevant signaling pathway protein expression level was measured via western blot assay. Signaling transduction inhibitor LY294002 was used to block PI3K/AKT/mTOR signaling pathway. Finally, in vivo the effect of miR-150-5p on embryonic absorption rate was evaluated in mice. Results Clinical samples revealed that miR-150-5p expression was significantly elevated in the villous tissues and serum of URSA patients. Moreover, the overexpressing of miR-150-5p could inhibit both HTR-8/SVneo cell and JAR cell migration, invasion, and restrained PI3K/AKT/mTOR signaling pathway by targeting VEGFA in vitro. This inhibitory effect of miR-150-5p could be reversed by overexpressing the gene of vascular epithelial growth factor A (VEGFA). In contrary, inhibition of miR-150-5p significantly enhanced migration, invasion ability of both HTR-8/SVneo and JAR cells, and also could stimulate PI3K/AKT/mTOR signaling pathway. This promoting effect of miR-150-5p could be ameliorated by LY294002 (PI3K inhibitor). Finally, after miR-150-5p overexpression in vivo, the embryo resorption rate in pregnant mice was increased significantly. Conclusions Overall, these findings imply that miR-150-5p is among the key factors that regulate the pathogenesis of URSA.

show abstract

miR-181d-5p, which is upregulated in fetal growth restriction placentas, inhibits trophoblast fusion via CREBRF

Wu,

Li,

Ruan

et al. 2023

J Assist Reprod Genet

View full text Add to dashboard Cite

miR-526b-5p/c-Myc/Foxp1 participates in recurrent spontaneous abortion by regulating the proliferation, migration, and invasion of trophoblasts

Cited by 5 publications

References 41 publications

Role of increased IGFBP2 in trophoblast cell proliferation and recurrent spontaneous abortion development: A pilot study

Role of increased IGFBP2 in trophoblast cell proliferation and recurrent spontaneous abortion development: A pilot study

MiR-150-5p contributes to unexplained recurrent spontaneous abortion by targeting VEGFA and downregulating the PI3K/AKT/mTOR signaling pathway

miR-181d-5p, which is upregulated in fetal growth restriction placentas, inhibits trophoblast fusion via CREBRF

Contact Info

Product

Resources

About