MiR-543 Promotes Proliferation and Epithelial-Mesenchymal Transition in Prostate Cancer via Targeting RKIP

Abstract

Background/Aims: MicroRNAs (miRNAs, miRs) have emerged as important post-transcriptional regulators in various cancers. miR-543 has been reported to play critical roles in hepatocellular carcinoma and colorectal cancer, however, the role of miR-543 in the pathogenesis of prostate cancer has not been fully understood. Methods: Expression of miR-543 and Raf Kinase Inhibitory Protein (RKIP) in clinical prostate cancer specimens, two prostate cancer cell lines, namely LNCAP and C4-2B, were determined. The effects of miR-543 on proliferation and metastasis of tumor cells were also investigated with both in vitro and in vivo studies. Results: miR-543 was found to be negatively correlated with RKIP expression in clinical tumor samples and was significantly upregulated in metastatic prostate cancer cell line C4-2B compared with parental LNCAP cells. Further studies identified RKIP as a direct target of miR-543. Overexpression of miR-543 downregulated RKIP expression and promoted the proliferation and metastasis of cancer cells, whereas knockdown of miR-543 increased expression of RKIP and suppressed the proliferation and metastasis of cancer cells in vitro and in vivo. Conclusion: Our study demonstrates that miR-543 promotes the proliferation and metastasis of prostate cancer via targeting RKIP.

MicroRNA-34a Attenuates Paclitaxel Resistance in Prostate Cancer Cells via Direct Suppression of JAG1/Notch1 Axis

“…By directly targeting protein-coding genes, miRNAs can inhibit genes that are needed for signaling pathways or drug-induced apoptosis [25]. In recent years, miRNAs have attracted attention in PC [26,27]. Multiple miRNAs are thought to be critical for the control of drug resistance in PC [25].…”

Circulating microRNA-34 family low expression correlates with poor prognosis in patients with non-small cell lung cancer

“…coding RNA molecules at length about 19 to 24 nucleotides, have established their value into multiple pathological processes, including cell proliferation, cell apoptosis and cell differentiation, through the post-transcriptional regulation of genes expression (6)(7)(8). Accumulating evidences disclose that several miRNAs act as important biomarkers for the diagnosis and prognosis of various cancers, such as NSCLC, pancreatic cancer and colorectal cancer (9)(10)(11).…”

Implications of microRNA dysregulation in the development of prostate cancer

“…RKIP downregulation by miR-543 in PCa cell lines increased invasion and migration in vitro and tumor proliferation and VIM/CDH1 ratio in vivo (Du et al 2017).…”

“…Reproduction (2017) 154 R81-R97 www.reproduction-online.org Another member of the miR-200s family, miR-141, was revealed to be a strong epithelial phenotype inducer by upregulation of CDH1, cytokeratins and many other epithelial markers, but caused partial loss of EMT, evidenced by downregulation of merely ZEB1 and VIM (Liu et al 2017).…”