miR‑590‑5p may regulate colorectal cancer cell viability and migration by targeting PDCD4

Abstract

Recent studies have revealed that microRNAs (miRs) are involved in the pathogenesis of colorectal cancer (CRC); however, the roles of miR-590-5p in CRC are not completely understood. Therefore, the present study investigated the expression of miR-590-5p and programmed cell death 4 (PDCD4) in CRC tissues and healthy adjacent tissues via reverse transcription-quantitative PCR. Furthermore, human CRC cells were cultured in vitro and transfected with miR-590-5p inhibitor. CRC cell viability, migration and invasion were evaluated by conducting MTT, wound healing and Transwell assays, respectively. Additionally, the relative expression of PDCD4 and phosphorylated-Smad2/3 was analyzed via western blotting. miR-590-5p was significantly upregulated and PDCD4 was significantly downregulated in CRC tissues compared with healthy adjacent tissues. Moreover, the results indicated that miR-590-5p knockdown inhibited cell viability and migration by altering the expression of PDCD4, transforming growth factor-β and phosphorylated-Smad2/3. PDCD4 was identified as a direct target of miR-590-5p. In conclusion, the results of the present study suggested that miR-590-5p may regulate CRC cell viability and migration, indicating that miR-590-5p may serve as a potential therapeutic target for CRC.

Expressions of miR-590 in Oral Lichen Planus and Oral Squamous Cell Carcinoma Tissues and Clinical Values

“…MiR-590 is located near the long arm of chromosome 7 of human genome, and its function remains elusive. Existing research has reported that miR-590 shows abnormal expressions in various tumors and diseases 15,16) . For example, Cai et al 17) identified that miR-590-5p was able to inhibit the mRNA expression of its target gene signal transducer and activator of transcription 3 (STAT3) through the luciferase reporter system, and that STAT3 was only expressed in osteosarcoma tissues and cells, exhibiting great effects in the invasion and infiltration of osteosarcoma cells.…”

Exploring the Key Signaling Pathways and ncRNAs in Colorectal Cancer

“…In particular, miRNAs influence major signaling pathways in CRC, as discussed in Section 2, resulting in changes in tumor phenotype [99]. For example, miR-590-5p was overexpressed in CRC patient tissues and cell lines and was verified to directly regulate the expression of PDCD4, thereby reducing its expression [100]. It was confirmed that the decreased miR-590-5p induced the expression of PDCD4, resulting in modulating the expression of TGF-β and SMAD2/3, genes involved in the TGF-β signaling pathway.…”

Noncoding RNAs as regulators of STAT3 pathway in gastrointestinal cancers: Roles in cancer progression and therapeutic response

“…338 Programmed cell death 4 (PDCD4) is a tumor-suppressor protein that can inhibit the progression of CRC cells. 339,340 The IL-6/STAT3 axis increases the expression of miR-181b which in turn reduces PDCD4 expression and prevents apoptotic cell death. Besides, IL-6/STAT3/miR-181b/PDCD4 axis increases metastasis of CRC cells.…”

“…Upregulation of IL‐17C is suggested to promote CRC progression through the activation of STAT3 signaling and subsequent elevation of miR‐23a‐3p expression which leads to angiogenesis induction via VEGF upregulation 338 . Programmed cell death 4 (PDCD4) is a tumor‐suppressor protein that can inhibit the progression of CRC cells 339,340 . The IL‐6/STAT3 axis increases the expression of miR‐181b which in turn reduces PDCD4 expression and prevents apoptotic cell death.…”