miR-597-3p inhibits invasion and migration of thyroid carcinoma SW579 cells by targeting RAB23

Wen, Hu‐Ling; Xu, Zhengmin; Lin, Shiyu; Wen, Dan; Xie, Jingwei

doi:10.5603/ep.a2020.0041

Cited by 8 publications

(4 citation statements)

References 21 publications

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“…We used mechanistic approach in this study which revealed that LINC01213 could exert its role on PRAD cells through miR-597-3p and BCL2L1 dependent pathway. Tumor inhibiting properties of miR-597-3p have been previously reported by Wen et al who have shown that this miRNA could inhibit the proliferation and invasion of thyroid carcinoma SW579 cells by modulating the expression of RAB23 [19]. In the current report, miR-597-3p overexpression adversely a affected the survival and proliferation of PRAD cell lines which is in corroboration of the report by Wen et al…”

Section: Discussionsupporting

confidence: 92%

LINC01213 promotes prostate cancer cell progression through miR-597/ BCL2L1 axis

Zhao

Wang

et al. 2021

Preprint

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Background: Majority of cancer related deaths in males are attributed to prostate cancer (PRAD) throughout the world. Recently, the role of long non-coding RNAs (lncRNAs) in the pathogenesis of cancer has been widely explored. In this study, we investigated the role of lncRNA LINC01213 (LINC01213) in tumorigenesis of prostate cancer (PRAD).Methods: PRAD and adjacent tissue samples were collected from cancer patients. Survival rate among these patients was compared by Kaplan–Meier analysis. PRAD cells viability was estimated by CCK-8 method while AnnexinV/PI cytometry assay was used to determine the percent of apoptotic cells. qRT-PCR and western blot assay were used to determine the mRNA and protein expressions, respectively. Interaction between LINC01213 and corresponding miRNA as well as between miRNA and mRNA was confirmed by dual luciferase reporter gene assay. PRAD cells were also injected subcutaneously in nude mice to support in vitro findings.Results: It was observed that LINC01213 was highly expressed in PRAD samples and cell lines. Down-regulation of LINC01213 in PRAD cells decreased cell viability and inhibited proliferation. Luciferase reporter gene assay and RNA pull-down confirmed that LINC01213 targeted miR-597-3p. Increased expression of miR-597-3p resulted in decreased BCL2L2 expression in vitro. Inhibitory effects of miR-597-3p on PRAD cells’ survival and growth were diminished after LINC01213 overexpression which was also associated with alteration in the protein expression of BCL-xL, BCL-2 as well as caspase 3 and caspase 9.Conclusion: Taken together, our findings suggest that LINC01213 plays its role in PRAD tumorigenesis through miR-597-3p/ BCL2L2 dependent pathway with associated modulation of genes involved in cell survival and apoptosis.

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Section: Discussionsupporting

confidence: 92%

LINC01213 promotes prostate cancer cell progression through miR-597/ BCL2L1 axis

Zhao

Wang

et al. 2021

Preprint

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“…Evidence exists reporting that miR-597-3p might function as a tumor suppressor (21), which intrigued us to examine the role of miR-597-3p in OS. The MTT assay indicated that the delivery of miR-597-3p mimic diminished OS cell viability, while its inhibitor had the opposite effect (Figures 4A, B).…”

Section: Lncrna Lbx2-as1 Regulates Os Cell Functions By Sponging Mir-...mentioning

confidence: 99%

LncRNA LBX2-AS1 impacts osteosarcoma sensitivity to JQ-1 by sequestering miR-597-3p away from BRD4

Yuan

et al. 2023

Front. Oncol.

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ObjectiveRecent knowledge concerning the significance of long non-coding RNA (lncRNA)-mediated ceRNA networks provides new insight into their possible roles as specific biomarkers for the treatment of osteosarcoma (OS). Thus, this study aims to clarify the functional relevance and mechanistic actions of lncRNA LBX2-AS1 in OS.MethodsDifferential analysis was performed by integrating the TCGA and GTEx databases. Cox regression analysis was then employed to assess the prognostic value of the model. The expression of lncRNA LBX2-AS1 and miR-597-3p was quantified in OS cell lines by qRT-PCR. The proliferation, migration, invasion, and apoptosis of OS cell lines in response to manipulated lncRNA LBX2-AS1 were evaluated by MTT, colony formation, transwell, Western blot, and flow cytometry assays. Luciferase activity was assayed to validate the reciprocal regulation between lncRNA LBX2-AS1 and miR-597-3p. The protein levels of BRD4 and EMT-related factors were examined by Western blot assay. Finally, tumor growth in response to LBX2-AS1 knockdown was evaluated in xenograft-bearing nude mice.ResultsBy integrating the GTEx and TCGA databases, we identified 153 differentially expressed lncRNAs. Among them, 5 lncRNAs, RP11-535M15.1, AC002398.12, RP3-355L5.4, LBX2-AS1, and RP11.47A8.5, were selected to establish a model, which predicted the prognosis of OS. Higher lncRNA LBX2-AS1 expression was noted in OS tissues relative to that in normal tissues. Silencing lncRNA LBX2-AS1 facilitated apoptosis and curtailed proliferative, migratory, and invasive capacities of OS cells. Mechanistically, lncRNA LBX2-AS1 could elevate the expression of BRD4, an oncogene, by competitively binding to miR-597-3p. More importantly, knockdown of lncRNA LBX2-AS1 increased the sensitivity of OS cells to the BRD4 inhibitor JQ-1. Finally, the tumor growth of OS cell xenografts was constrained in vivo in the presence of lncRNA LBX2-AS1 knockdown.ConclusionIn conclusion, lncRNA LBX2-AS1 promotes the growth of OS and represses the sensitivity to JQ-1 by sponging miR-597-3p to elevate the expression of BRD4.

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“…The miR-34/449-5p (Position: 413-419 3ʹUTR, Chr: 1), which is a target of SDHC gene is involved in causing head and neck cancer [34]. The miR-802 (Position: 29-36 3ʹUTR, Chr: 11), which is a target of SDHD is reported to be linked with the suppression of gastric cancer [35]. The miR-17-5p (Position:515-522 3ʹUTR, Chr: 2), which is a target of TMEM127 gene is linked with the hepatocellular carcinoma [36].…”

Section: Avrupa Bilim Ve Teknoloji Dergisi E-issn: 2148-2683 1494 Mirnas and Regulated Target Genes Cardiac Hypertrophymentioning

confidence: 99%

MicroRNAs in molecular technology to address global diseases bench to bedside research

Akram¹,

Shahzor²,

Mushtaq³

et al. 2021

European Journal of Science and Technology

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miR-597-3p inhibits invasion and migration of thyroid carcinoma SW579 cells by targeting RAB23

Abstract: miR-597-3p inhibits invasion and migration of thyroid carcinoma SW579 cells by targeting RAB23 Running title: miR-597-3p inhibits thyroid carcinoma SW579 cells

Cited by 8 publications

References 21 publications

LINC01213 promotes prostate cancer cell progression through miR-597/ BCL2L1 axis

LINC01213 promotes prostate cancer cell progression through miR-597/ BCL2L1 axis

LncRNA LBX2-AS1 impacts osteosarcoma sensitivity to JQ-1 by sequestering miR-597-3p away from BRD4

MicroRNAs in molecular technology to address global diseases bench to bedside research

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