miR‐615‐5p is restrictedly expressed in cirrhotic and cancerous liver tissues and its overexpression alleviates the tumorigenic effects in hepatocellular carcinoma

Tayebi, H.M. El; Hosny, Karim Adel; Esmat, Gamal; Breuhahn, Kai; Abdelaziz, Ahmed Ihab

doi:10.1016/j.febslet.2012.06.054

Cited by 50 publications

(43 citation statements)

References 25 publications

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“…A ratio of ~2.0 is generally accepted as 'pure' for RNA. Thus, RNA samples with absorbance values outside the range of 1.8-2 were excluded from the study, as previously described (20,25 (20,25).…”

Section: Methodsmentioning

confidence: 99%

“…Subsequent to incubation for 5 h, formazan (MTT metabolic product) was re-suspended in 200 µl dimethyl sulfoxide. Colorimetric measurements and absorbance were performed using a Wallac 1420 Victor2 Multilabel Counter (Perkin Elmer Inc., Waltham, MA, USA), as previously described (20,25).…”

Section: Methodsmentioning

confidence: 99%

“…At 48 h post-oligonucleotide transfection, the cells were labeled with BrdU labeling reagent for 4 h (with a final concentration of 100 µM) using the Cell Proliferation ELISA kit (Roche Applied Science, Penzberg, Germany). The cells were then fixed using FixDenate for 30 min and incubated with Anti-BrdU POD (with a final concentration of 10 µM) for 90 min, as previously described (20,25).…”

Section: Methodsmentioning

confidence: 99%

“…This drug is based around miRNA-34a, which is one of the most prominent tumor suppressor miRNAs in HCC (19). The miRNA of other potential tumor suppressors has been found to target several components of the IGF-signaling pathway; for example, miR-615-5p, which was previously reported by our group to directly target IGF-2 (20). Focusing on the gatekeeper of the IGF-axis, IGF-1R, several miRNAs, including miR-145 and miR-99a, have been found to act as tumor suppressors in HCC through direct targeting of IGF-1R (21,22).…”

Section: Microrna-486-5p Enhances Hepatocellular Carcinoma Tumor Suppmentioning

confidence: 97%

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MicroRNA-486-5p enhances hepatocellular carcinoma tumor suppression through repression of IGF-1R and its downstream mTOR, STAT3 and c-Myc

et al. 2016

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Microrna-486-5p Enhances Hepatocellular Carcinoma Tumor Suppmentioning

confidence: 97%

See 2 more Smart Citations

MicroRNA-486-5p enhances hepatocellular carcinoma tumor suppression through repression of IGF-1R and its downstream mTOR, STAT3 and c-Myc

et al. 2016

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“…The potential regulation of IGF axis members by microRNAs is an appealing subject of investigation. We have previously shown that miR-615-5p downregulates IGF-Ⅱ expression and forcing its expression reduces tumorigenesis in HCC [14] . miR-122 was found to suppress IGF-1R expression thus inhibiting HCC progression [15,16] .…”

Section: Introductionmentioning

confidence: 99%

Interplay between microRNA-17-5p, insulin-like growth factor-II through binding protein-3 in hepatocellular carcinoma

Habashy¹,

Tayebi²,

Fawzy³

et al. 2016

WJH

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AIM:To investigate the effect of microRNA on insulinlike growth factor binding protein-3 (IGFBP-3) and hence on insulin-like growth factor-Ⅱ (IGF-Ⅱ) bioavailability in hepatocellular carcinoma (HCC). METHODS:Bioinformatic analysis was performed using microrna.org, DIANA lab and Segal lab softwares. Total RNA was extracted from 23 HCC and 10 healthy liver tissues using mirVana miRNA Isolation Kit. microRNA-17-5p (miR-17-5p) expression was mimicked and antagonized in HuH-7 cell lines using HiPerFect Basic Study 977August 18, 2016|Volume 8|Issue 23| WJH|www.wjgnet.comHabashy DA et al . miR-17-5p regulates IGF-Ⅱ via targeting IGFBP-3 Transfection Reagent, then total RNA was extracted using Biozol reagent then reverse transcribed into cDNA followed by quantification of miR-17-5p and IGFBP-3 expression using TaqMan real-time quantitative PCR. Luciferase reporter assay was performed to validate the binding of miR-17-5p to the 3'UTR of IGFBP-3. Free IGF-Ⅱ protein was measured in transfected HuH-7 cells using IGF-Ⅱ ELISA kit. RESULTS:Bioinformatic analysis revealed IGFBP-3 as a potential target for miR-17-5p. Screening of miR-17-5p and IGFBP-3 revealed a moderate negative correlation in HCC patients, where miR-17-5p was extensively underexpressed in HCC tissues (P = 0.0012), while IGFBP-3 showed significant upregulation in the same set of patients (P = 0.0041) compared to healthy donors. Forcing miR-17-5p expression in HuH-7 cell lines showed a significant downregulation of IGFBP-3 mRNA expression (P = 0.0267) and a significant increase in free IGF-Ⅱ protein (P = 0.0339) compared to mock untransfected cells using unpaired t -test. Luciferase assay validated IGFBP-3 as a direct target of miR-17-5p; luciferase activity was inhibited by 27.5% in cells co-transfected with miR-17-5p mimics and the construct harboring the wild-type binding region 2 of IGFBP-3 compared to cells transfected with this construct alone (P = 0.0474). CONCLUSION:These data suggest that regulating IGF-Ⅱ bioavailability and hence HCC progression can be achieved through targeting IGFBP-3 via manipulating the expression of miRNAs.

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Noncoding RNAs within the HOX gene network in tumor pathogenesis and progression

Botti

Chiara

Bonito

et al. 2018

Journal Cellular Physiology

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HOX genes are involved with normal development, cell identity, cell differentiation, cell metabolism, apoptosis, autophagy as well as with diseases such as tumor pathogenesis and progression. In particular, the genes belonging to HOX paralogous 13 seem to carry out a relevant role in both tumor development and disease progression. In recent years, several noncoding RNAs (ncRNA) sequences have been identified in HOX loci, including long noncoding RNA (lncRNA) and microRNA (miRNA), highly conserved during evolution. Many studies have shown that specific intergenic ncRNAs in HOX loci could directly modulate HOX genes expression in normal and pathological conditions. In the present review we attempt to describe the role of these ncRNAs, through the regulation of the HOX gene network, in normal cell biology, and, with particular emphasis, in diseases such as in cancer pathogenesis and progression.

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miR‐615‐5p is restrictedly expressed in cirrhotic and cancerous liver tissues and its overexpression alleviates the tumorigenic effects in hepatocellular carcinoma

Cited by 50 publications

References 25 publications

MicroRNA-486-5p enhances hepatocellular carcinoma tumor suppression through repression of IGF-1R and its downstream mTOR, STAT3 and c-Myc

MicroRNA-486-5p enhances hepatocellular carcinoma tumor suppression through repression of IGF-1R and its downstream mTOR, STAT3 and c-Myc

Interplay between microRNA-17-5p, insulin-like growth factor-II through binding protein-3 in hepatocellular carcinoma

Noncoding RNAs within the HOX gene network in tumor pathogenesis and progression

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