MiR-624-5p enhances NLRP3 augmented gemcitabine resistance via EMT/IL-1β/Wnt/β-catenin signaling pathway in ovarian cancer

Alrashed, May; Alharbi, Homood; Alshehry, Abdualrahman Saeed; Ahmad, Mohammad; aloahd, Mustafa sawsan

doi:10.1016/j.jri.2022.103488

Cited by 14 publications

(8 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been shown that miR-542-3p promotes the rapid degradation of PTGS2 mRNA, which partly supports our bioinformatics prediction [32]. MiR-624-5p [33][34][35] and miR-873-5p [36][37][38] have been studied in cancer, but their role in DR has not been investigated. According to the analysis of published literature, relevant TFs PPARG [39,40], STAT1 [41], AR [42], SP1 [43], STAT3 [44], RELA [45] and EGR1 [46] are closely related to DR.…”

Section: Discussionsupporting

confidence: 82%

Identification of key ferroptosis genes in diabetic retinopathy based on bioinformatics analysis

2023

View full text Add to dashboard Cite

Objectives Diabetic retinopathy (DR) is a retinal microvascular disease associated with diabetes. Ferroptosis is a new type of programmed cell death that may participate in the occurrence and development of DR. Therefore, this study aimed to identify the DR ferroptosis-related genes by bioinformatics methods. Methods The RNAseq data of DR and healthy control retinas were downloaded from the gene expression synthesis (GEO) database and analyzed using the R package DESeq2. The key modules were obtained using the WGCNA algorithm, and their genes were intersected with ferroptosis-related genes in the FerrDb database to obtain differentially expressed ferroptosis-related genes (DE-FRGs). Enrichment analysis was conducted to understand the function and enrichment pathways of ferroptosis genes in DR, and hub genes were identified by protein-protein interaction (PPI) analysis. The diagnostic accuracy of hub genes for DR was evaluated according to the area under the ROC curve. The TRRUST database was then used to predict the regulatory relationship between transcription factors and target genes, with the mirDIP, ENCORI, RNAnter, RNA22, miRWalk and miRDB databases used to predict the regulatory relationship between miRNAs and target genes. Finally, another data set was used to verify the hub genes. Results In total, 52 ferroptosis-related DEGs (43 up-regulated and 9 down-regulated) were identified using 15 DR samples and 3 control samples and were shown to be significantly enriched in the intrinsic apoptotic signaling pathway, autophagosome, iron ion binding and p53 signaling pathway. Seven hub genes of DR ferroptosis were identified through PPI network analysis, but only HMOX1 and PTGS2 were differentially expressed in another data set. The miRNAs prediction showed that hsa-miR-873-5p was the key miRNA regulating HMOX1, while hsa-miR-624-5p and hsa-miR-542-3p were the key miRNAs regulating PTGS2. Furthermore, HMOX1 and PTGS2 were regulated by 13 and 20 transcription factors, respectively. Conclusion The hub genes HMOX1 and PTGS2, and their associated transcription factors and miRNAs, may be involved in ferroptosis in diabetic retinopathy. Therefore, the specific mechanism is worthy of further investigation.

show abstract

Section: Discussionsupporting

confidence: 82%

Identification of key ferroptosis genes in diabetic retinopathy based on bioinformatics analysis

2023

View full text Add to dashboard Cite

show abstract

“…Moreover, small RNA can also regulate the Wnt/β-catenin signaling pathway to promote or inhibit the production of tumor resistance to chemotherapeutic drugs. For example, MiR-624-5p upregulates nod-like receptor family pyrin domain-containing protein 3 (NLRP3) expression through the EMT/IL-1/Wnt/β-catenin signaling pathway and promotes gemcitabine resistance in ovarian cancer [ 98 ]. MiR-331-3p activates Wnt/β-catenin through the suppressor of tumorigenicity 7 protein-like (ST7L) and promotes resistance to chemotherapeutic drugs in prostate cancer [ 99 ].…”

Section: The Signaling Pathways In Tumor Drug Resistancementioning

confidence: 99%

Natural Products in Preventing Tumor Drug Resistance and Related Signaling Pathways

et al. 2022

View full text Add to dashboard Cite

Drug resistance is still an obstacle in cancer therapy, leading to the failure of tumor treatment. The emergence of tumor drug resistance has always been a main concern of oncologists. Therefore, overcoming tumor drug resistance and looking for new strategies for tumor treatment is a major focus in the field of tumor research. Natural products serve as effective substances against drug resistance because of their diverse chemical structures and pharmacological effects. We reviewed the signaling pathways involved in the development of tumor drug resistance, including Epidermal growth factor receptor (EGFR), Renin-angiotensin system (Ras), Phosphatidylinositol-3-kinase/protein kinase B (PI3K/Akt), Wnt, Notch, Transforming growth factor-beta (TGF-β), and their specific signaling pathway inhibitors derived from natural products. This can provide new ideas for the prevention of drug resistance in cancer therapy.

show abstract

“…214 NLRP3 is involved in ovarian cancer chemoresistance with downregulation increasing drug sensitivity in gemcitabine-resistant cell lines and overexpression inducing IL-1β, EMT, and Wnt/β-catenin signaling. 215 Carboplatin therapy increases the activation of the NLRP3 inflammasome in macrophages, indicating NLRP3 implications for ovarian cancer and potential chemoresistance. 216 NLRP1 inflammasome-induced pyroptosis was exacerbated by the inhibition of lncRNA HOTTIP, which suppressed ovarian cancer.…”

Section: Ovarian Cancermentioning

confidence: 99%

“…Moreover, it has been proven to be safe and therapeutically effective in Phase I clinical trials in recurrent ovarian cancer 214 . NLRP3 is involved in ovarian cancer chemoresistance with downregulation increasing drug sensitivity in gemcitabine‐resistant cell lines and overexpression inducing IL‐1β, EMT, and Wnt/β‐catenin signaling 215 . Carboplatin therapy increases the activation of the NLRP3 inflammasome in macrophages, indicating NLRP3 implications for ovarian cancer and potential chemoresistance 216 …”

Section: Pyroptosis and Inflammasomes In Cancermentioning

confidence: 99%

Pyroptosis and inflammasomes in cancer and inflammation

Wang,

Hua,

Bao

et al. 2023

MedComm

View full text Add to dashboard Cite

Nonprogrammed cell death (NPCD) and programmed cell death (PCD) are two types of cell death. Cell death is significantly linked to tumor development, medication resistance, cancer recurrence, and metastatic dissemination. Therefore, a comprehensive understanding of cell death is essential for the treatment of cancer. Pyroptosis is a kind of PCD distinct from autophagy and apoptosis in terms of the structure and function of cells. The defining features of pyroptosis include the release of an inflammatory cascade reaction and the expulsion of lysosomes, inflammatory mediators, and other cellular substances from within the cell. Additionally, it displays variations in osmotic pressure both within and outside the cell. Pyroptosis, as evidenced by a growing body of research, is critical for controlling the development of inflammatory diseases and cancer. In this paper, we reviewed the current level of knowledge on the mechanism of pyroptosis and inflammasomes and their connection to cancer and inflammatory diseases. This article presents a theoretical framework for investigating the potential of therapeutic targets in cancer and inflammatory diseases, overcoming medication resistance, establishing nanomedicines associated with pyroptosis, and developing risk prediction models in refractory cancer. Given the link between pyroptosis and the emergence of cancer and inflammatory diseases, pyroptosis‐targeted treatments may be a cutting‐edge treatment strategy.

show abstract

MiR-624-5p enhances NLRP3 augmented gemcitabine resistance via EMT/IL-1β/Wnt/β-catenin signaling pathway in ovarian cancer

Cited by 14 publications

References 18 publications

Identification of key ferroptosis genes in diabetic retinopathy based on bioinformatics analysis

Identification of key ferroptosis genes in diabetic retinopathy based on bioinformatics analysis

Natural Products in Preventing Tumor Drug Resistance and Related Signaling Pathways

Pyroptosis and inflammasomes in cancer and inflammation

Contact Info

Product

Resources

About