miR‑671‑3p is downregulated in non‑small cell lung cancer and inhibits cancer progression by directly targeting CCND2

Abstract

MicroRNAs (miRNAs) are implicated in the development and progression of non-small cell lung cancer (NSCLC). A previous study suggested that miR-671-3p suppresses the development of breast cancer. However, the role of miR-671-3p in NSCLC remains largely unknown. In the present study, it was identified that miR-671-3p was significantly upregulated in NSCLC tissues compared with adjacent normal tissues by reverse transcription quantitative polymerase chain reaction (RT-qPCR). Similarly, decreased levels of miR-671-3p in NSCLC cell lines were observed compared with those in the non-tumorigenic human bronchial epithelial NL20 cell line. Cell Counting Kit-8 and Transwell invasion assays indicated that miR-671-3p overexpression suppressed the proliferation and invasion of A549 cells, and vice versa. Mechanistically, it was demonstrated that CCND2 was a direct target of miR-671-3p. RT-qPCR and western blot analysis indicated that miR-671-3p overexpression decreased the expression of CCND2 in A549 cells. Furthermore, rescue experiments demonstrated that the restoration of CCND2 may significantly reverse the suppressive roles of miR-671-3p overexpression on NSCLC cell proliferation and invasion. Taken together, the present study demonstrated that miR-671-3p exerted its tumor-suppressive roles via directly targeting CCND2 in NSCLC.

CircTHBS1 targeting miR-211/CCND2 pathway to promote cell proliferation and migration potential in primary cystitis glandularis cells

“…At the same time, HUANG et al reported that miR-615 inhibits the proliferation and invasion of prostate cancer cells by targeted down-regulation of CCND2. In addition, miR-1297 [32], miR-154, and miR-497 [33] have been reported to target down-regulation of CCND2 to inhibit tumor cell proliferation and migration [34][35][36][37]. For this reason, the present study speculates and…”

LncRNA-PACERR induces pro-tumour macrophages via interacting with miR-671-3p and m6A-reader IGF2BP2 in pancreatic ductal adenocarcinoma

“…The effect of miR-671-3p on tumours is still controversial and mostly based on studies of tumour cells [ 24 , 49 ]. The role of miR-671-3p has not been reported in TME.…”

CircRNA circ_0092314 Induces Epithelial-Mesenchymal Transition of Pancreatic Cancer Cells via Elevating the Expression of S100P by Sponging miR-671

“…Downregulation of miR-671 has been observed in a panel of human tumors, such as osteosarcoma (23), prostate cancer (24), lung cancer (25), breast cancer (26), and gastric cancer (27). In these tumors, miR-671 was demonstrated to show inhibit the malignant phenotypes of tumor cells by targeting SOX6, CCND2, and FOXM1 (23)(24)(25)(26)(27). However, miR-671 may function as an oncogenic miRNA in other types of cancer (28,29).…”