miR‑7‑5p inhibits cell migration and invasion in glioblastoma through targeting SATB1

Yin, Changyou; Kong, Wei; Jiang, Jing; Xu, Hao; Zhao, Wei

doi:10.3892/ol.2018.9777

Cited by 34 publications

(37 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Zhu et al demonstrated that miR-7-5p suppresses cell migration and invasion by targeting SOX18 in pancreatic ductal adenocarcinoma [26]. MiR-7-5p has been found to target and regulate the genes as SATB1 and PARP1 in some cancer diseases as we have mentioned before [27,28]. MiR-7-5p may have several target genes similar to miR-137; such target genes are critical oncogenic factors, including TGFβ2, that could further regulate brain tumorigenesis [29].…”

Section: Discussionmentioning

confidence: 88%

Identification of Differentially Expressed microRNAs in Metastatic Ovarian Cancer

Zhang

2020

Preprint

View full text Add to dashboard Cite

Background: The molecular mechanisms of ovarian cancer (OC) remain unclear. We sought to comprehensively identify microRNAs (miRNAs) that are aberrantly expressed in metastatic OC. Methods: Differentially expressed miRNAs were screened from six pairs of primary and metastatic OC tissues; their possible functions were analyzed and target genes were predicted by bioinformatics. Then gene expression profiling results were established by reverse transcription quantitative polymerase chain reaction and western blot. Targeting relationship between miR-7-5p and TGFβ2 was validated by dual-luciferase reporter assay. CCK-8, Transwell assay, scratch test and flow cytometry were used for cell function detection after miR-7-5p overexpression. Results: Twelve miRNAs and 10 target mRNAs were differentially expressed in primary and metastatic OC tissues. ITGB3, TGFβ2 and TNC correlated to miRNA function in metastatic OC. Among all 7 miRNAs, expression of hsa-miR-141-3p, hsa-miR-7-5p, hsa-miR-187-5p, hsa-miR-200a-3p, and hsa-miR-200b-3p in metastatic OC tissues was obviously lower than that in primary OC tissues ( p < 0.05). Moreover, there was a significant correlation between hsa-miR-7-5p and TGFβ2 in OC tissues. Dual-luciferase reporter assay confirmed that hsa-miR-7-5p negatively targeted TGFβ2. After miR-7-5p overexpression, the OC cell viability and invasion were reduced, the cell cycle was blocked ( p < 0.05). Conclusions: Hsa-miR-141-3p, hsa-miR-187-5p, hsa-miR-7-5p, hsa-miR-200a-3p, and hsa-miR-200b-3p expression was prominently lower in metastatic OC than in primary OC, while TGFβ2 expression was markedly increased in metastatic OC tissues. Hsa-miR-7-5p bound to TGFβ2 3’-UTR to inhibit its expression. Hsa-miR-7-5p targeted TGFβ2 to inhibit cell proliferation, invasion and cell cycle entry.

show abstract

Section: Discussionmentioning

confidence: 88%

Identification of Differentially Expressed microRNAs in Metastatic Ovarian Cancer

Zhang

2020

Preprint

View full text Add to dashboard Cite

show abstract

“…miR-214-3p plays an important role in depressive-like behaviours, cognition defects, Alzheimer's disease, and endothelial cell dysfunction and mediates neural apoptosis, neuropathic pain, and the growth, migration, and invasion of glioma cells [60][61][62][63][64][65]. miR-7-5p is relevant to vascular endothelial cell proliferation, glioma, brain damage, and cognitive dysfunction [66][67][68][69]. Moreover, it has been reported that miR-7-5p boosts apoptosis but inhibits cell proliferation and apoptosis of T lymphocytes, explaining the function of miR-214-3p and miR-7-5p in psoriasis [70,71].…”

Section: Journal Of Immunology Researchmentioning

confidence: 99%

Plasma MicroRNA Expression Profiles in Psoriasis

Xiao

Liu

Wang

et al. 2020

Journal of Immunology Research

View full text Add to dashboard Cite

Background. Psoriasis is an immune-mediated inflammatory chronic skin disease characterized by chronic inflammation in the dermis, parakeratosis, and excessive epidermal growth. MicroRNAs (miRNAs) are key regulators of immune responses. Although differential expression of miRNAs has been reported in certain inflammatory autoimmune diseases, their role in psoriasis has not been fully illuminated. Our aims were to confirm plasma miRNA expression signatures in psoriasis and to examine their potential influence on psoriasis pathogenesis. Methods. A miRNome PCR array was used to analyse the plasma of psoriasis patients and healthy donors. We performed miRNA pathway enrichment and target gene network analyses on psoriasis plasma samples. Results. We found several specific plasma miRNA signatures relevant to psoriasis. The miRNAs targeted pathways associated with psoriasis, such as the VEGF, MAPK, and WNT signaling pathways. Network analysis revealed pivotal deregulated plasma miRNAs and their relevant target genes and pathways regulating psoriasis pathogenesis. Conclusions. This study analysed the expression of plasma miRNAs and their target pathways, elucidating the pathogenesis of psoriasis; these results may be used to design novel therapeutic strategies and to identify diagnostic biomarkers for psoriasis.

show abstract

“…This type of regulation may lead to a novel manner of inhibiting the expression of pathogenic genes that could potentially be used in small RNA drug development. MIR-7 was first discovered as a tumor suppressor, which achieved anti-tumor effects by directly targeting tumor growth-promoting genes or modifying regulatory tumor suppressor factors [49][50][51][52][53][54]. In addition to anti-tumor effects, miR-7 also undergoes expression changes after viral infection and plays a role in antiviral defense by regulating gene expression and related pathways in host cells.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-7 Inhibits Rotavirus Replication by Targeting Viral NSP5 In Vivo and In Vitro

Zhou

Chen

et al. 2020

Viruses

View full text Add to dashboard Cite

Rotavirus (RV) is the major causes of severe diarrhea in infants and young children under five years of age. There are no effective drugs for the treatment of rotavirus in addition to preventive live attenuated vaccine. Recent evidence demonstrates that microRNAs (miRNAs) can affect RNA virus replication. However, the antiviral effect of miRNAs during rotavirus replication are largely unknown. Here, we determined that miR-7 is upregulated during RV replication and that it targets the RV NSP5 (Nonstructural protein 5). Results suggested that miR-7 affected viroplasm formation and inhibited RV replication by down-regulating RV NSP5 expression. Up-regulation of miR-7 expression is a common regulation method of different G-type RV-infected host cells. Then, we further revealed the antiviral effect of miR-7 in diarrhea suckling mice model. MiR-7 is able to inhibit rotavirus replication in vitro and in vivo. These data provide that understanding the role of cellular miR-7 during rotaviral replication may help in the identification of novel therapeutic small RNA molecule drug for anti-rotavirus.

show abstract

miR‑7‑5p inhibits cell migration and invasion in glioblastoma through targeting SATB1

Cited by 34 publications

References 33 publications

Identification of Differentially Expressed microRNAs in Metastatic Ovarian Cancer

Identification of Differentially Expressed microRNAs in Metastatic Ovarian Cancer

Plasma MicroRNA Expression Profiles in Psoriasis

MicroRNA-7 Inhibits Rotavirus Replication by Targeting Viral NSP5 In Vivo and In Vitro

Contact Info

Product

Resources

About