miR-744–5p mediates lncRNA HOTTIP to regulate the proliferation and apoptosis of papillary thyroid carcinoma cells

Yuan, Qingling; Fan, Yuxia; Liu, Zheng; Wang, Xiaoming; Meng, Jia; Geng, Zushi; Zheng, Jiayi; Lu, Xiaoyan

doi:10.1016/j.yexcr.2020.112024

Cited by 13 publications

(8 citation statements)

References 22 publications

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“…Taking these findings together with ours, it is evident that the low expression or abnormally high expression of miR-744 leads to opposite effects on NSCLC cells. Recent studies have found that miR-744 has a strong anti-cancer effect in thyroid cancer and liver cancer [31,32], which is consistent with our findings. Therefore, the effect of miR-744 on the function of NSCLC cells is worthy of further exploration.…”

Section: Discussionsupporting

confidence: 93%

Reduction of miR-744 delivered by NSCLC cell-derived extracellular vesicles upregulates SUV39H1 to promote NSCLC progression via activation of the Smad9/BMP9 axis

Gao

Tian

et al. 2021

J Transl Med

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Background Non-small cell lung cancer (NSCLC) is a common type of lung cancer. Extracellular vehicles (EVs) are nano-sized particles containing proteins, lipids, and miRNAs secreted by various cells, which play important roles in the development of lung cancer by regulating a wide range of signaling pathways. This study focused on elucidating a potential mechanism by which EVs promote the development of NSCLC. Methods Expression levels of miR-744, SUV39H1, Smad9, and BMP4 in clinical tissue samples of NSCLC patients and cell lines were quantified by RT-qPCR and/or western blot analysis. The interaction between SUV39H1 and miR-744 was identified by dual-luciferase reporter assay. miR-744, SUV39H1, and BMP4 expression levels were modulated in A549 and H460 cells, in order to evaluate their effects on cell proliferation, colony formation and cell cycle. A NSCLC xenograft mouse model was used to verify the in vitro findings. NSCLC cell-derived EVs and normal bronchial epithelial cell-derived EVs were isolated and their roles in NSCLC development were evaluated in vivo and in vitro. Results miR-744 expression was lower in cancer cell-derived derived EVs than in normal lung epithelial cell-derived EVs. Reduced miR-744 expression in EVs upregulated SUV39H1 in NSCLC cells and further increased BMP4 levels to promote NSCLC development. BMP4 was upregulated in NSCLC cells upon suppression of Smad9 mediated by SUV39H1. Reduced miR-744 expression transferred from cancer cell-derived EVs into NSCLC cells enhanced cancer development. Conclusion Overall, our findings unveiled a mechanism whereby miR-744 delivered by NSCLC-derived EVs upregulated SUV39H1, activating the Smad9/BMP9 axis and thus promoted cancer development.

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Section: Discussionsupporting

confidence: 93%

Reduction of miR-744 delivered by NSCLC cell-derived extracellular vesicles upregulates SUV39H1 to promote NSCLC progression via activation of the Smad9/BMP9 axis

Gao

Tian

et al. 2021

J Transl Med

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“…These results indicated that miR-744-5p partially inhibited the progression of CRC by downregulating SEPT2. Notably, recent studies reported that miR-744-5p targets c-Myc in papillary thyroid carcinoma cells (30), Heterogeneous Nuclear Ribonucleoprotein C (HNRNPC) and Nuclear Factor I X (NFIX) in ovarian cancer cells (19), and Paired box gene 2 (PAX2) in non-small cell lung cancer (NSCLC) (20). To determine the correlation between miR-744-5p and the aforementioned gene expression, the expression change of HNRNPC, NFIX, c-Myc and PAX2 in CRC tissues and normal tissues was examined via TCGA-COAD database.…”

Section: Sept2 Is Upregulated In Crc and Is Inversely Correlated Withmentioning

confidence: 99%

MicroRNA‑744‑5p is downregulated in colorectal cancer and targets SEPT2 to suppress the malignant phenotype

2020

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MicroRNA (miR)-744-5p serves a pivotal role in the progression of multiple cancers; however, the function of miR-744-5p in colorectal cancer (CRC) remains largely unknown. In the present study, the effects of miR-744-5p on the progression of CRC were analyzed and the mechanisms involved were investigated. It was revealed that miR-744-5p was frequently downregulated in CRC tissues and cell lines. Overexpression of miR-744-5p significantly inhibited the proliferation, colony formation, and promoted the apoptosis of CRC cells. Bioinformatics analysis revealed that Septin 2 (SEPT2) was a potential target of miR-744-5p. miR-744-5p bound the 3′-untranslated region (UTR) of SEPT2 and reduced the level of SEPT2 in CRC cells. A negative correlation between the expression of miR-744-5p and SEPT2 was observed in CRC tissues. Overexpression of SEPT2 counteracted the suppressive effect of miR-744-5p on the proliferation and apoptosis of CRC cells. Collectively, these data demonstrated the functional mechanism of miR-744-5p by targeting SEPT2, which suggested miR-744-5p as a potential target for the treatment of patients with CRC.

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“…This ultimately leads to elevated apoptosis. These studies suggest that HOTTIP is also a typical oncogenic lncRNA and may be an important therapeutic target in PTC (61).…”

Section: Hottipmentioning

confidence: 81%

Long non-coding RNA signatures as predictors of prognosis in thyroid cancer: a narrative review

Zhao¹,

Souza²,

Kumar³

et al. 2021

Ann Transl Med

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Thyroid cancer (TC) is the most common endocrine malignancy, with high incidence rates in recent decades. Most TC cases have good prognoses, but a high risk of recurrence and metastases poses challenges, especially for patients with high-risk factors. Currently used prognostic markers for TC involve a combination of genetic factors and overexpressed proteins. Long non-coding RNAs (lncRNAs) regulate several integral biologic processes by playing key roles in the transcription of several downstream targets maintaining cellular behavior. Prior studies have revealed that lncRNAs promote tumor cell proliferation, invasion, metastasis, and angiogenesis, making them important targets for therapeutic intervention in cancer.While the exact molecular mechanisms underlying the role of lncRNAs in modulating TC progression and recurrence is still unclear, it is important to note that some lncRNAs are upregulated in certain cancers, while others are downregulated. In the present study, we review several key lncRNAs, their association with cancer progression, and the important roles they may play as tumor suppressors or tumor promoters in tumorigenesis. We discuss the potential mechanisms of lncRNA-mediated pathogenesis that can be targeted for the treatment of TC, the existing and potential benefits of using lncRNAs as diagnostic and prognostic measures for cancer detection, and tumor burden in patients.

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miR-744–5p mediates lncRNA HOTTIP to regulate the proliferation and apoptosis of papillary thyroid carcinoma cells

Cited by 13 publications

References 22 publications

Reduction of miR-744 delivered by NSCLC cell-derived extracellular vesicles upregulates SUV39H1 to promote NSCLC progression via activation of the Smad9/BMP9 axis

Reduction of miR-744 delivered by NSCLC cell-derived extracellular vesicles upregulates SUV39H1 to promote NSCLC progression via activation of the Smad9/BMP9 axis

MicroRNA‑744‑5p is downregulated in colorectal cancer and targets SEPT2 to suppress the malignant phenotype

Long non-coding RNA signatures as predictors of prognosis in thyroid cancer: a narrative review

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