miR-874 ameliorates retinopathy in diabetic rats by NF-κB signaling pathway

Abstract

Background: This study aimed to explore the improvement effect of miR-874 on retinopathy in diabetic rats by NF-κB signaling pathway. Methods: Ten healthy Sprague-Dawley rats were taken as the control group. Sixty streptozotocin (60 mg/kg)-induced diabetes model rats were randomly divided into model group (without any treatmentinjection of normal saline), negative control (NC) agomir group (injection of overexpressed NC vector), miR-874 agomir group (injection of miR-874 mimic), miR-874 anti-agomir group (injection of miR-874 inhibitor), EVP4593 group (injection of NF-κB signaling pathway antagonist EVP4593), and miR-874 anti-agomir + EVP4593 group (injection of miR-874 inhibitor and EVP4593). All injection was via caudal vein. Results: miR-874 could target the degradation of p65. Compared with control group, there were significantly reduced miR-874 expression, increased VEGF and Ang2 protein expressions, lowered end-diastolic velocity and peak systolic velocity of central retinal artery (CRA), and blood velocity of central retinal vein and CRA, heightened plasma viscosity, blood viscosity and erythrocyte sedimentation rate at all shear rates, decreased capillary pericytes, increased vascular endothelial cells, and ascended p65 expression in the retina of rats in model group (all P < 0.05). It showed that pathological changes were happened on the retina of diabetes rats. These indexes showed the same results after miR-874 was inhibited (all P < 0.05). However, these indexes showed the opposite results in miR-874 agomir group and EVP4593 group compared with model group (all P < 0.05). EVP4593 could alleviate the aggravation of retinopathy caused by the inhibition of miR-874 in diabetes rats. Conclusions: miR-874 mediates NF-κB signaling pathway by targeting the degradation of p65 to further improve the retina of diabetes rats, showing the improvement effect of miR-874 on diabetic retinopathy in rats.

A Pilot Study on MicroRNA Profile in Tear Fluid to Predict Response to Anti-VEGF Treatments for Diabetic Macular Edema

“…Increased expression of miR-320a and miR-320b has been identified in eyes with proliferative diabetic retinopathy [ 63 ], but this is the first report of an association between miR-320d and diabetic retinopathy. MiR-874 has been found to alleviate diabetic retinopathy in a murine model by regulating the NF-κB signaling pathway [ 64 ], ameliorating renal injury in diabetic nephropathy [ 65 ], and providing neuroprotection in a stroke model [ 66 ].…”