MiR-876-5p suppresses epithelial–mesenchymal transition of lung cancer by directly down-regulating bone morphogenetic protein 4

Bao, Lei; Lv, Lei; Feng, Jinping; Chen, Yuyu; Wang, Xinhua; Han, Song; Zhao, Hongqing

doi:10.1007/s12038-017-9722-5

Cited by 35 publications

(41 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MiR‐876‐5p restricted the aggressive behaviors of hepatocellular carcinoma (HCC) . MiR‐876‐5p had suppressive activity against lung cancer . Nevertheless, miR‐876's effect on GC has yet to be studied.…”

Section: Introductionmentioning

confidence: 99%

MiR‐876‐3p regulates cisplatin resistance and stem cell‐like properties of gastric cancer cells by targeting TMED3

Peng

Huang

Liu

et al. 2019

J of Gastro and Hepatol

View full text Add to dashboard Cite

Background and Aim Gastric cancer (GC), a prevalent tumor, exerts a major economic burden, and we aimed to explore miR‐876‐3p's effects on GC and related mechanisms. Methods Cell viability was analyzed via CCK‐8 and colony formation assay. Stem cell‐like properties were examined via spheroid colony formation assay. mRNA abundance of key genes was analyzed via quantitative polymerase chain reaction. Protein level of TMED3 and stem cell markers was examined by western blot. TargetScan, luciferase, and biotin‐miRNA pulldown assay were used to identify miR‐876‐3p's target. Results MiR‐876‐3p was downregulated in GC, and its mRNA level had negative relationship with cisplatin resistance of GC. Moreover, decreased miR‐876‐3p expression level suggested poor prognosis of GC patients. MiR‐876‐3p inhibited drug resistance of cisplatin‐resistant cell line SGC‐7901/DDP and MKN‐45/DDP, as shown by decreased cell viability, IC50, and colony formation ability. MiR‐876‐3p inhibited stem cell‐like features and downregulated the expressions of Sox‐2, Oct‐4, CD133, and CD44 in GC cells. Luciferase and biotin‐miRNA pulldown assay confirmed that TMED3 was miR‐876‐3p's direct target. TMED3 siRNA inhibited miR‐876‐3p's effects on cisplatin resistance and stem cell‐like features of SGC‐7901/DDP cells. Conclusion MiR‐876‐3p enhanced cisplatin sensitivity and restricted stem cell‐like features of GC through targeting TMED3.

show abstract

Section: Introductionmentioning

confidence: 99%

MiR‐876‐3p regulates cisplatin resistance and stem cell‐like properties of gastric cancer cells by targeting TMED3

Peng

Huang

Liu

et al. 2019

J of Gastro and Hepatol

View full text Add to dashboard Cite

show abstract

“…That data suggest a tumour suppressive role for miR‐876‐5p in cancer progression. Indeed, this tumour suppressive function is evidenced in a wide range of cancers, including lung cancer, esophageal squamous cell carcinoma, head and neck squamous cell carcinoma, hepatocellular carcinoma, gastric cancer, and breast cancer . However, whether miR‐876‐5p is involved in colorectal cancer remains undetermined.…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, this tumour suppressive function is evidenced in a wide range of cancers, including lung cancer, esophageal squamous cell carcinoma, head and neck squamous cell carcinoma, hepatocellular carcinoma, gastric cancer, and breast cancer. 28,29,[33][34][35][36] However, whether miR-876-5p is involved in colorectal cancer remains undetermined. Here, our results demonstrated that miR-876-5p was decreased in colorectal cancer.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‐876‐5p represses the cell proliferation and invasion of colorectal cancer through suppressing YAP signalling via targeting RASAL2

Ren

Zhang

Feng

et al. 2020

Clin Exp Pharma Physio

View full text Add to dashboard Cite

Aberrant expression of microRNA‐876‐5p (miR‐876‐5p) is implicated in the progression of multiple human cancers. However, the potential role of miR‐876‐5p in colorectal cancer remains poorly understood. The purpose of the current study was to investigate the potential role of miR‐876‐5p in colorectal cancer. miR‐876‐5p expression was significantly downregulated in colorectal cancer tissues and cell lines compared with normal controls. Gain‐of‐function assays revealed that miR‐876‐5p overexpression effectively repressed the malignant behaviours of colorectal cancer cells, including cell proliferation, colony formation, and invasion. Bioinformatics analysis predicted that RAS protein activator like 2 (RASAL2), a potential oncogene for colorectal cancer, is a putative miR‐876‐5p target gene. A luciferase reporter assay confirmed that miR‐876‐5p directly binds to the 3′‐untranslated region (UTR) of RASAL2. Furthermore, both RASAL2 messenger RNA (mRNA) and protein expression were negatively modulated by miR‐876‐5p in colorectal cancer cells. Notably, there was an inverse correlation between miR‐876‐5p and RASAL2 expression in colorectal cancer tissue specimens. Moreover, miR‐876‐5p was involved in regulating the activation of Yes‐associated protein (YAP) signalling through inhibiting RASAL2. However, the miR‐876‐5p‐mediated antitumour effect on colorectal cancer cells was partially reversed by restoring RASAL2 expression. Notably, miR‐876‐5p upregulation impeded the tumour growth of colorectal cancer cells in vivo in nude mice. Overall, these results demonstrated that miR‐876‐5p exerts an antitumour function in colorectal cancer by targeting RASAL2 to suppress YAP signalling activation. These findings highlight the importance of the miR‐876‐5p/RASAL2/YAP axis in colorectal cancer progression and suggest that miR‐876‐5p is a potential therapeutic target for treating colorectal cancer.

show abstract

“…11 MiR-613 inhibits OS cell proliferation and migration, and induces apoptosis by targeting chemokine receptor 4 (CXCR4). 13 Recently, A new tumour-suppressive miR-876-5p has been found to play important roles in a variety of tumours including lung cancer, 14 oesophageal squamous cell carcinoma (ESCC), 15 hepatocellular carcinoma (HCC) 16,17 and head and neck squamous cell carcinoma (HNSCC). 13 Recently, A new tumour-suppressive miR-876-5p has been found to play important roles in a variety of tumours including lung cancer, 14 oesophageal squamous cell carcinoma (ESCC), 15 hepatocellular carcinoma (HCC) 16,17 and head and neck squamous cell carcinoma (HNSCC).…”

mentioning

confidence: 99%

“…13 Recently, A new tumour-suppressive miR-876-5p has been found to play important roles in a variety of tumours including lung cancer, 14 oesophageal squamous cell carcinoma (ESCC), 15 hepatocellular carcinoma (HCC) 16,17 and head and neck squamous cell carcinoma (HNSCC). 14,16,18 Moreover, miR-876-5p suppresses tumour growth and metastasis of ESCC by targeting tumour antigen MAGE-A family. 14,16,18 Moreover, miR-876-5p suppresses tumour growth and metastasis of ESCC by targeting tumour antigen MAGE-A family.…”

mentioning

confidence: 99%

MicroRNA‐876‐5p inhibits cell proliferation, migration and invasion by targeting c‐Met in osteosarcoma

Xie

Xiao

Wang

et al. 2019

J Cellular Molecular Medi

View full text Add to dashboard Cite

Recently, aberrant expression of miR‐876‐5p has been reported to participate in the progression of several human cancers. However, the expression and function of miR‐876‐5p in osteosarcoma (OS) are still unknown. Here, we found that the expression of miR‐876‐5p was significantly down‐regulated in OS tissues compared to para‐cancerous tissues. Clinical association analysis indicated that underexpression of miR‐876‐5p was positively correlated with advanced clinical stage and poor differentiation. More importantly, OS patients with low miR‐876‐5p level had a significant shorter overall survival compared to miR‐876‐5p high‐expressing patients. In addition, gain‐ and loss‐of‐function experiments demonstrated that miR‐876‐5p restoration suppressed whereas miR‐876‐5p knockdown promoted cell proliferation, migration and invasion in both U2OS and MG63 cells. In vivo studies revealed that miR‐876‐5p overexpression inhibited tumour growth of OS in mice. Mechanistically, miR‐876‐5p reduced c‐Met abundance in OS cells and inversely correlated c‐Met expression in OS tissues. Herein, c‐Met was recognized as a direct target of miR‐876‐5p using luciferase reporter assay. Notably, c‐Met restoration rescued miR‐876‐5p attenuated the proliferation, migration and invasion of OS cells. In conclusion, these findings indicate that miR‐876‐5p may be used as a potential therapeutic target and promising biomarker for the diagnosis and prognosis of OS.

show abstract

MiR-876-5p suppresses epithelial–mesenchymal transition of lung cancer by directly down-regulating bone morphogenetic protein 4

Cited by 35 publications

References 36 publications

MiR‐876‐3p regulates cisplatin resistance and stem cell‐like properties of gastric cancer cells by targeting TMED3

MiR‐876‐3p regulates cisplatin resistance and stem cell‐like properties of gastric cancer cells by targeting TMED3

MicroRNA‐876‐5p represses the cell proliferation and invasion of colorectal cancer through suppressing YAP signalling via targeting RASAL2

MicroRNA‐876‐5p inhibits cell proliferation, migration and invasion by targeting c‐Met in osteosarcoma

Contact Info

Product

Resources

About