miR-885-5p Negatively Regulates Warburg Effect by Silencing Hexokinase 2 in Liver Cancer

Xu, Fei; Yan, Jingjun; Gan, Yun; Chang, Ying; Wang, Hongling; He, Xingxing; Zhao, Qiu

doi:10.1016/j.omtn.2019.09.002

Cited by 63 publications

(52 citation statements)

References 37 publications

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“…Multiple pathological factors participate in the tumor progression of BC, moreover, circRNA could regulate the series of tumor phenotype, including metastasis and Warburg effect 20 . Warburg effect, also known as the aerobic glycolysis, provides the major energy for the BC tumor microenvironment 21,22 .…”

Section: Discussionmentioning

confidence: 99%

Circular RNA circRNF20 promotes breast cancer tumorigenesis and Warburg effect through miR-487a/HIF-1α/HK2

et al. 2020

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Compelling evidence has demonstrated the potential functions of circular RNAs (circRNAs) in breast cancer (BC) tumorigenesis. Nevertheless, the underlying mechanism by which circRNAs regulate BC progression is still unclear. The purpose of present research was to investigate the novel circRNA circRNF20 (hsa_circ_0087784) and its role in BC. CircRNA microarray sequencing revealed that circRNF20 was one of the upregulated transcripts in BC samples. Increased circRNF20 level predicted the poor clinical outcome in BC specimens. Functionally, circRNF20 promoted the proliferation and Warburg effect (aerobic glycolysis) of BC cells. Mechanistically, circRNF20 harbor miR-487a, acting as miRNA sponge, and then miR-487a targeted the 3'-UTR of hypoxia-inducible factor-1α (HIF-1α). Moreover, HIF-1α could bind with the promoter of hexokinase II (HK2) and promoted its transcription. In conclusion, this finding illustrates the vital roles of circRNF20 via the circRNF20/ miR-487a/HIF-1α/HK2 axis in breast cancer progress and Warburg effect, providing an interesting insight for the BC tumorigenesis.

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Section: Discussionmentioning

confidence: 99%

Circular RNA circRNF20 promotes breast cancer tumorigenesis and Warburg effect through miR-487a/HIF-1α/HK2

et al. 2020

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“…Most of the research articles in the literature have con rmed that miR-885-5p plays diverse roles in different cancers. For instance, miR-885-5p acted as a tumor promoter and tumor suppressor in hepatocellular carcinoma and colorectal cancer, respectively [17,18]. While the overexpression of miR-885-5p impeded cell invasion in glioma cells [19,20], researchers are yet to investigate the upstream regulator of miR-885-5p in glioblastoma.…”

mentioning

confidence: 99%

LncRNA HOXA-AS2 promotes glioblastoma carcinogenesis by targeting miR-885-5p/RBBP4 axis

Shou

Gao

Cheng

et al. 2020

Preprint

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Background: LncRNA HOXA-AS2 has been found in the literature to deteriorate glioblastoma. However, its regulatory mechanism is yet to be fully investigated. Our study focused chiefly on the interaction and role of the HOXA-AS2/miR-885-5p/RBBP4 axis in the development of glioblastoma. Methods: qRT-PCR analysis was performed to detect the expression of lncRNA, miRNA and mRNA in glioblastoma tissues and cells. Dual-luciferase assay, RIP assay and RNA pull-down assay were later carried out to reveal the interactions among HOXA-AS2, miR-885-5p and RBBP4. After that, CCK-8 assay, BrdU assay, nude mice xenografting assay, western blot assay, and flow cytometry were carried out to analyze the effect of the HOXA-AS2/miR-885-5p/RBBP4 axis on glioblastoma samples. Results: HOXA-AS2 and RBBP4 were found to be overexpressed in glioblastoma. Experimental results showed that HOXA-AS2 and RBBP4 contributed to the tumorigenesis of glioblastoma cells. However, miR-885-5p was observed to be downregulated in glioblastoma. Findings also indicated that HOXA-AS2 could negatively regulate miR-885-5p, thereby enhancing RBBP4 expression. Conclusion: Overall, HOXA-AS2 promoted the tumorigenesis of glioblastoma by targeting and regulating miR-885-5p to induce the expression of RBBP4.

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“…The prediction was supported by Argonaute-crosslinking immunoprecipitation sequencing (AGO-CLIPseq) data[32,33]. HK2 is a definite hypoxia-inducible gene[34]. The above results suggest that the high expression of HK2 in hypoxia may be associated with the reduction in miR-216b-5p.…”

mentioning

confidence: 65%

“…have been frequently reported [34,40,41] In recent years, the molecular classification of HCC has received attention. We used the 21-gene hypoxia signature to perform subtype analysis on HCC patients.…”

Section: Discussionmentioning

confidence: 99%

A novel hypoxia gene signature indicates prognosis and reveals the multi-omics molecular landscape of tumour tissue in patients with hepatocellular carcinoma

Zhang

Liao²,

Qiao

et al. 2020

Preprint

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Background: Previous studies on the impact of hypoxia on hepatocellular carcinoma (HCC) mostly focused on in vitro and animal models. The clinical value of assessing the degree of hypoxia in in vivo tissues and hypoxia-related molecular landscapes in HCC remain poorly defined. Methods: A novel hypoxia gene signature was extracted from hypoxia-treated HCC cells using microarray analysis and a robust rank aggregation algorithm and was verified using public data. Next, the relationships between the hypoxia gene signature and the clinical characteristics and prognoses of HCC patients were analysed. Based on the multi-omics data from The Cancer Genome Atlas-Liver Hepatocellular Carcinoma (TCGA-LIHC) and 10 independent HCC cohorts from the Gene Expression Omnibus (GEO), a comprehensive analysis was conducted using the hypoxia gene signature to describe hypoxia-associated multi-omic molecular landscapes and immune microenvironments in HCC tissues. Results: A 21-gene hypoxia signature was constructed to effectively indicate the exposure of hypoxia in HCC tissues. This novel hypoxia signature and the hypoxia scores calculated based on this signature were closely correlated to some clinical characteristics of HCC patients and can be used to evaluate prognosis. HCC tissues with different hypoxia scores differed significantly in transcriptomic, genomic, epigenomic, and proteomic alterations and immune microenvironments, some of which were related to the clinical prognoses of patients. Conclusion: The 21-gene signature can effectively estimate hypoxia exposure of HCC tissues and has clinical value in the assessment and prediction of the prognosis of HCC patients. Using this 21-gene signature, hypoxia-associated molecular landscapes were described at the tissue level. This comprehensive molecular-level understanding can help to further elucidate the mechanism of hypoxia in tumours and guide clinical cancer therapy. The assessment of the degree of hypoxia is strongly recommended in the personalized treatment of HCC patients to benefit specific patient groups.

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miR-885-5p Negatively Regulates Warburg Effect by Silencing Hexokinase 2 in Liver Cancer

Cited by 63 publications

References 37 publications

Circular RNA circRNF20 promotes breast cancer tumorigenesis and Warburg effect through miR-487a/HIF-1α/HK2

Circular RNA circRNF20 promotes breast cancer tumorigenesis and Warburg effect through miR-487a/HIF-1α/HK2

LncRNA HOXA-AS2 promotes glioblastoma carcinogenesis by targeting miR-885-5p/RBBP4 axis

A novel hypoxia gene signature indicates prognosis and reveals the multi-omics molecular landscape of tumour tissue in patients with hepatocellular carcinoma

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