MiR-886-5p Inhibition Inhibits Growth and Induces Apoptosis of MCF7 Cells

Abstract

Background and Aims: To explore the molecular mechanisms of miR-886-5p in breast cancer., we examined roles in inhibiting growth and migration of MCF-7 cells. Methods: MiR-886-5p mimics and inhibitors were used to express or inhibit MiR-886-5p, respectively, and MTT and clone formation assays were used to determine the survival and proliferation. Hoechst 33342/ PI double staining was applied to detect apoptosis. The expression of caspase-3, caspase-8, caspase-9, MT1-MMP, VEGF-C and VEGF-D was detected by Western blotting, and the levels of MMP2 and MMP9 secreted from MCF-7 cells were assessed by ELISA. MCF-7 cell migration was determined by wound healing and Transwell assays. Results: We found that the growth of MCF-7 cells was inhibited upon decreasing miR-886-5p levels. Inhibiting miR-866-5p also significantly induced apoptosis and decreased the migratory capacity of these cells. The expression of VEGF-C, VEGF-D, MT1-MMP, MMP2, and MMP9 was also found to be decreased as compared to controls. Conclusions: Our data show that downregulation of miR-886-5p expression in MCF-7 cells could significantly inhibit cell growth and migration. This might imply that inhibiting miR-886-5p could be a therapeutic strategy in breast cancer.

vtRNA2-1/nc886 Produces a Small RNA That Contributes to Its Tumor Suppression Action through the microRNA Pathway in Prostate Cancer

“…Indeed, snc886-3p enrichment is above the average of the microRNAs in these experiments, indicating a specialized microRNA-like function for this small RNA product in comparison to the 5p. Although our study shows a low association of snc886-5p to AGO it does not rule out the function of snc886-5p as a microRNA, which has been shown previously in other tissues (brain [19], cervical [66,67], breast [68]). Alternatively, snc886-5p might be involved in another pathway or be a non-functional secondary product of nc886 processing.…”

Therapeutic effects of tyroserleutide on lung metastasis of human hepatocellular carcinoma SK-HEP-1 and its mechanism affecting ICAM-1 and MMP-2 and -9

“…The relative expression of proteins was determined as MMP/β-actin densitometric ratio through using TotalLab software. 8 …”

Breast Cancer: Major Risk Factors and Recent Developments in Treatment

“…The levels of VEGF MMP2 and MMP9 were also reduced by reducing the expression of miR-886-5p. From results it was concluded that miR-886-5p inhibitors can be used as therapeutic agents in treatment of breast cancer as they caused a significant reduction in growth and migration of MCF-7 cells (Zhang et al, 2014).…”