miR-941 as a promising biomarker for acute coronary syndrome

Abstract

BackgroundCirculating miRNAs can function as biomarkers for diagnosis, treatment, and prevention of diseases. However, it is unclear whether miRNAs can be used as biomarkers for acute coronary syndrome (ACS). To this end, we applied gene chip technology to analyze miRNA expression in patients with stable angina (SA), non-ST elevation ACS (NSTE-ACS), and ST-segment elevation myocardial infarction (STEMI).MethodsWe enrolled patients with chest pain who underwent diagnostic coronary angiography, including five patients each with SA, NSTE-ACS, or STEMI, and five controls without coronary artery disease (CAD) but with three or more risk factors. After microarray analysis, differential miRNA expression was confirmed by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR).ResultsCompared with those in patients with STEMI, differentially expressed microRNAs in controls and patients with SA or NSTE-ACS were involved in inflammation, protein phosphorylation, and cell adhesion. Pathway analysis showed that differentially expressed miRNAs were related to the mitogen-activated protein kinase signaling, calcium ion pathways, and cell adhesion pathways. Compared with their expression levels in patients with STEMI, miR-941, miR-363-3p, and miR-182-5p were significantly up-regulated (fold-change: 2.0 or more, P < 0.05) in controls and patients with SA or NSTE-ACS. Further, qRT-PCR showed that plasma miR-941 level was elevated in patients with NSTE-ACS or STEMI as compared with that in patients without CAD (fold-change: 1.65 and 2.28, respectively; P < 0.05). Additionally, miR-941 expression was significantly elevated in the STEMI group compared with that in the SA (P < 0.01) and NSTE-ACS groups (P < 0.05). Similarly, miR-941 expression was higher in patients with ACS (NSTE-ACS or STEMI) than in patients without ACS (without CAD or with SA; P < 0.01). There were no significant differences in miR-182-5p and miR-363-3p expression. The areas under the receiver operating characteristic curves were 0.896, 0.808, and 0.781 for patients in the control, SA, and NSTE-ACS groups, respectively, compared with that for patients with STEMI; that for the ACS group compared with the non-ACS group was 0.734.Conclusion miR-941 expression was relatively higher in patients with ACS and STEMI. Thus, miR-941 may be a potential biomarker of ACS or STEMI.Electronic supplementary materialThe online version of this article (doi:10.1186/s12872-017-0653-8) contains supplementary material, which is available to authorized users.

Differential Secretion of Angiopoietic Factors and Expression of MicroRNA in Umbilical Cord Blood from Healthy Appropriate-For-Gestational-Age Preterm and Term Newborns—in Search of Biomarkers of Angiogenesis-Related Processes in Preterm Birth

“…Our observation of lower expression levels of the above-mentioned angiogenesis-specific subset of miRNAs, which have been identified as crucial regulators of vascular biology and endothelial cell function, may indicate their potential angiogenesis-regulating role in premature gestation coupled with angiopoietic proteins imbalance in UCB found in this study. In this notion, the recent bioinformatics analyses have shown that miR-941, which appeared to be the most down-regulated microRNA in preterm newborns in our study, may be associated with metabolism, inflammation, cell proliferation, and other biological processes through regulation of components involved in insulin signaling, MAPK signaling cascade, and other pathways related pathophysiologically to coronary heart disease and atherosclerosis [82]. However, no research has yet been reported on the role of miR-941 in angiogenesis in course of preterm gestation.…”

Keap1-targeting microRNA-941 protects endometrial cells from oxygen and glucose deprivation-re-oxygenation via activation of Nrf2 signaling

“…Functionally, miR-941 negatively regulated KDM6B (lysine (K)-specific demethylase 6B) to inhibit HCC cell epithelialmesenchymal transition (EMT) and cell migration/invasion [57]. Bai et al, reported that miR-941 levels are significantly higher in acute coronary syndrome patients than those in healthy controls [58].…”

Keap1-targeting microRNA-941 protects endometrial cells from oxygen and glucose deprivation-re-oxygenation via activation of Nrf2 signaling

“…Functionally, miR-941 negatively regulated KDM6B (lysine (K)-specific demethylase 6B) to inhibit HCC cell epithelial-mesenchymal transition (EMT) and cell migration/invasion [57]. Bai et al, reported that miR-941 levels are significantly higher in acute coronary syndrome patients than those in healthy controls [58].…”