miR-99a-5p inhibits target gene FZD5 expression and steroid hormone secretion from goat ovarian granulosa cells

Zhu, Lu; Jing, Jing; Qin, Shuaiqi; Lu, Jiani; Zhu, Cuiyun; Zheng, Qi; LIU, Ya; Fang, Fugui; LI, Yun-sheng; Zhang, Yunhai; Ling, Yinghui

doi:10.1016/s2095-3119(21)63766-8

Cited by 7 publications

(3 citation statements)

References 28 publications

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“…28 miR-99a-5p suppressed the expression of the target gene frizzled class receptor 5 (FZD5) and thus blocked steroid hormone secretion in goat granulosa cells. 29 miR-130a-3p targets the prostate transmembrane protein androgen induced 1 (PMEPA1) gene to inhibit estradiol and progesterone synthesis in goat granulosa cells. 30 However, there are relatively few studies on miRNA modulation of steroidogenesis in chicken granulosa cells, and our results will further enrich the understanding of miRNA regulation.…”

Section: Discussionmentioning

confidence: 99%

“…miR-202–5p restrained lipid deposition and steroidogenesis in goose granulosa cells through targeting the acyl-CoA synthetase long-chain family member 3 ( ACSL3 ) gene . miR-99a-5p suppressed the expression of the target gene frizzled class receptor 5 ( FZD5 ) and thus blocked steroid hormone secretion in goat granulosa cells . miR-130a-3p targets the prostate transmembrane protein androgen induced 1 ( PMEPA1 ) gene to inhibit estradiol and progesterone synthesis in goat granulosa cells .…”

Section: Discussionmentioning

confidence: 99%

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Lipid Deposition and Progesterone Synthesis Are Increased by miR-181b-5p through RAP1B/ERK1/2 Pathway in Chicken Granulosa Cells

Lin,

Gong,

Sun

et al. 2023

J. Agric. Food Chem.

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Steroid hormones secreted by granulosa cells are essential for maintaining normal development of chicken follicles. Our previous sequencing data indicated that miR-181b-5p and RAS-related protein 1B (RAP1B) appeared to function in chicken granulosa cells, which was further explored in this study. The results suggested that miR-181b-5p facilitated the aggregation of lipid droplets and the synthesis of progesterone. In contrast, RAP1B astricted lipid deposition and progesterone secretion. Cotransfection of the RAP1B overexpression vector with miR-181b-5p mimic eliminated the promoting effect of miR-181b-5p. Dual-luciferase reporter assay confirmed that miR-181b-5p bound directly to the 3′ untranslated region (3′ UTR) of RAP1B. We also found that miR-181b-5p and RAP1B reduced and enhanced the phosphorylation levels of extracellular signal-regulated kinases 1 and 2 (ERK1/ 2), respectively. The application of ERK1/2 activators and inhibitors demonstrated that ERK1/2 is a negative regulator of lipid deposition and progesterone synthesis. In conclusion, we revealed that miR-181b-5p accelerated lipid deposition and progesterone synthesis through the RAP1B/ERK1/2 pathway in chicken granulosa cells. miR-181b-5p and RAP1B may serve as new biomarkers in breeding to improve chicken reproductive performance and prevent ovary-related diseases.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Lipid Deposition and Progesterone Synthesis Are Increased by miR-181b-5p through RAP1B/ERK1/2 Pathway in Chicken Granulosa Cells

Lin,

Gong,

Sun

et al. 2023

J. Agric. Food Chem.

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show abstract

“…MiRNAs are a group of small non-coding RNAs that mediate RNA silencing. They are involved in most encoded transcriptional proteins and are involved in all developmental and pathological processes in animals [ 6 , 7 , 8 ]. Many studies have shown that the regulation of the biological function by multiple miRNAs or miRNA families is synergistic [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Fatty Acid Desaturation Is Suppressed in Mir-26a/b Knockout Goat Mammary Epithelial Cells by Upregulating INSIG1

Zhu

Jiao

Gao

et al. 2023

IJMS

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MicroRNA-26 (miR-26a and miR-26b) plays a critical role in lipid metabolism, but its endogenous regulatory mechanism in fatty acid metabolism is not clear in goat mammary epithelial cells (GMECs). GMECs with the simultaneous knockout of miR-26a and miR-26b were obtained using the CRISPR/Cas9 system with four sgRNAs. In knockout GMECs, the contents of triglyceride, cholesterol, lipid droplets, and unsaturated fatty acid (UFA) were significantly reduced, and the expression of genes related to fatty acid metabolism was decreased, but the expression level of miR-26 target insulin-induced gene 1 (INSIG1) was significantly increased. Interestingly, the content of UFA in miR-26a and miR-26b simultaneous knockout GMECs was significantly lower than that in wild-type GMECs and miR-26a- and miR-26b-alone knockout cells. After decreasing INSIG1 expression in knockout cells, the contents of triglycerides, cholesterol, lipid droplets, and UFAs were restored, respectively. Our studies demonstrate that the knockout of miR-26a/b suppressed fatty acid desaturation by upregulating the target INSIG1. This provides reference methods and data for studying the functions of miRNA families and using miRNAs to regulate mammary fatty acid synthesis.

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MicroRNA-370-5p inhibits pigmentation and cell proliferation by downregulating mitogen-activated protein kinase kinase kinase 8 expression in sheep melanocytes

Ru-jun

WANG

et al. 2023

Journal of Integrative Agriculture

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miR-99a-5p inhibits target gene FZD5 expression and steroid hormone secretion from goat ovarian granulosa cells

Cited by 7 publications

References 28 publications

Lipid Deposition and Progesterone Synthesis Are Increased by miR-181b-5p through RAP1B/ERK1/2 Pathway in Chicken Granulosa Cells

Lipid Deposition and Progesterone Synthesis Are Increased by miR-181b-5p through RAP1B/ERK1/2 Pathway in Chicken Granulosa Cells

Fatty Acid Desaturation Is Suppressed in Mir-26a/b Knockout Goat Mammary Epithelial Cells by Upregulating INSIG1

MicroRNA-370-5p inhibits pigmentation and cell proliferation by downregulating mitogen-activated protein kinase kinase kinase 8 expression in sheep melanocytes

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