miR168 targets Argonaute1A mediated miRNAs regulation pathways in response to potassium deficiency stress in tomato

Abstract

Background Potassium (K+) is an essential ion for most plants, as it is involved in the regulation of growth and development. K+ homeostasis in plant cells has evolved to facilitate plant adaptation to K+-deficiency stress. Argonaute1 (AGO1) is regulated by miR168 to modulate the small RNA regulatory pathway by RNA silencing complex (RISC) in tomatoes. However, the role of miR168-mediated regulation of AGO1 in the context of K+ deficiency stress in tomatoes has not been elucidated yet. Results SlmiR168 and its target gene SlAGO1A were differentially expressed among low-K+-tolerant JZ34 and low-K+-sensitive JZ18 tomato plants. Transgenic tomato plants constitutively expressing pri-SlmiR168a showed stronger root system growth, better leaves development, and higher K+ contents in roots under K+-deficiency stress than those of the transgenic tomato lines expressing rSlAGO1A (SlmiR168-resistant) and the wild type (WT). Deep sequencing analysis showed that 62 known microRNAs (miRNAs) were up-regulated in 35S:rSlAGO1 compared with WT tomatoes. The same miRNAs were down-regulated in 35S:SlmiR168a compared with WT plants. The integrated analysis found 12 miRNA/mRNA pairs from the 62 miRNAs, including the root growth and cytokinin (CTK)/abscisic acid (ABA) pathways. Conclusions The regulation mediated by SlmiR168 of SlAGO1A contributes to the plant development under low-K+ stress. Moreover, this regulation mechanism may influence downstream miRNA pathways in response to low-K+ stress through the CTK/ABA and root growth modulation pathways.

CRISPR‐Cas9 mediated OsMIR168a knockout reveals its pleiotropy in rice

“…A protoplast cell‐based reporter assay further confirmed that these OsAGO1 family genes including OsAGO1c are targeted for mRNA cleavage by OsMIR168a . Our conclusion is consistent with previous studies in other plant species (Iki et al ., 2018; Li et al ., 2012; Liu et al ., 2020b; Xian et al ., 2014) and a recent study in rice (Wang et al ., 2021a). The phenotypes of the mature plants and seeds of the OsMIR168a rice mutants are also similar with the transgenic rice lines that carried the MIM168a construct (Wang et al ., 2021a).…”

“…It regulates plant growth and development (Vaucheret et al ., 2004; Wang et al ., 2021a; Xian et al ., 2014; Zhou et al ., 2020). It has also been found to participate in plant response to abiotic stress (Li et al ., 2012; Liu et al ., 2020b) and biotic stress such as virus (Iki et al ., 2018; Varallyay et al ., 2010) and fungus (Li et al ., 2014; Wang et al ., 2021a). MIR168’s broader roles in plants can be explained by the fact that it targets Argonaute 1 (AGO1) which encodes the core component of the RISC (Mallory et al ., 2008; Vaucheret, 2008; Voinnet, 2009).…”

Integrated Analysis of miRNAs Associated With Sugarcane Responses to Low-Potassium Stress

“…The roots are the primary source of K + uptake in plants ( Liu et al, 2020 ), but only a small proportion of the potassium present within the soil is in the form of available K + , and as such, K + deficiency has become a major global threat to sustainable agricultural planting efforts ( Römheld and Kirkby, 2010 ; Wang and Wu, 2015 ; Ye Z. et al, 2020 ). It is therefore vital that the genetics and regulation of plant low-K + stress responses be studied in detail to facilitate the development of more K + -efficient crop cultivars.…”

“…Argonaute1 (AGO1) is an RNA splicing mediator that is suppressed by miR-168 ( Qi et al, 2005 ; Xian et al, 2014 ). When exposed to low-K + stress, tomato ( Solanum lycopersicum ) plants exhibit the upregulation of SlmiR-168a and corresponding AGO1 downregulation that alters miRNA responses and bolsters K + deficiency tolerance ( Liu et al, 2020 ).…”

Correction to: miR168 targets Argonaute1A mediated miRNAs regulation pathways in response to potassium deficiency stress in tomato

“…In the original publication [ 1 ] there was an incorrect reference citation. In this correction article the incorrect and correct information are listed.…”