Mir363-3p attenuates post-stroke depressive-like behaviors in middle-aged female rats

Panta, Aditya; Pandey, Sivani; Duncan, Irma N.; Duhamel, Shaelynn; Sohrabji, Farida

doi:10.1016/j.bbi.2019.01.003

Cited by 26 publications

(27 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This test was performed as described in our previous study (Panta et al, 2019 ). Briefly, a three-chambered Plexiglass box was used where each chamber had a dimension of 40″ × 13″ and all chambers had open access to each other.…”

Section: Methodsmentioning

confidence: 99%

“…This test was performed as described in our previous study (Panta et al, 2019 ). This test consisted of three phases: habituation, familiarization, and test phase.…”

Section: Methodsmentioning

confidence: 99%

“…This test consisted of three phases: habituation, familiarization, and test phase. In the habituation phase, the rat was placed in a 16″ × 16″ open field and allowed to freely explore the arena for 10 min each day for 2 days as described in (Panta et al, 2019 ). On the third day (familiarization phase), the animal was again placed in the open-field apparatus, which now contained two identical objects (A + A) placed diagonally from each other.…”

Section: Methodsmentioning

confidence: 99%

“…In our stroke studies, mir363-3p mimics reduced both caspase-3 expression and activity in the ischemic brain (Selvamani and Sohrabji, 2017 ). Subsequently, we reported that mir363-3p treatment significantly reduced the depressive phenotype that develops in the chronic phase (30–90 days) after stroke (Panta et al, 2019 ). In this study, we show that mir363-3p treatment also improves long term cognitive impairment induced by stroke.…”

Section: Introductionmentioning

confidence: 96%

“…Our previous studies showed that in the chronic phase (1–3 months later) after MCAo, middle-aged female rats exhibited depressive-like symptoms in the form of anhedonia, increased “despair,” and decreased social interaction (Panta et al, 2019 ). This was accompanied by transiently elevated levels of inflammatory cytokines and lower circulating levels of Brain-derived neurotrophic factor, and attrition of the projection pathway between midbrain neurons of the ventral tegmental area/substantia nigra to the striatum (Panta et al, 2019 ). However, non-spatial memory as assessed by the Novel Object Recognition Task (NORT) was not impaired after MCAo at 3 months.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Mir363-3p Treatment Attenuates Long-Term Cognitive Deficits Precipitated by an Ischemic Stroke in Middle-Aged Female Rats

Panta

Montgomery

Nicolas

et al. 2020

Front. Aging Neurosci.

Self Cite

View full text Add to dashboard Cite

Cognitive impairment and memory loss are commonly seen after stroke and a third of patients will develop signs of dementia a year after stroke. Despite a large number of studies on the beneficial effects of neuroprotectants, few studies have examined the effects of these compounds/interventions on long-term cognitive impairment. Our previous work showed that the microRNA mir363-3p reduced infarct volume and sensory-motor impairment in the acute stage of stroke in middle-aged females but not males. Thus, the present study determined the impact of mir363-3p treatment on stroke-induced cognitive impairment in middle-aged females. Sprague–Dawley female rats (12 months of age) were subjected to middle cerebral artery occlusion (MCAo; or sham surgery) and injected (iv) with mir363-3p mimic (MCAo + mir363-3p) or scrambled oligos (MCAo + scrambled) 4 h later. Sensory-motor performance was assessed in the acute phase (2–5 days after stroke), while all other behaviors were tested 6 months after MCAo (18 months of age). Cognitive function was assessed by the novel object recognition test (declarative memory) and the Barnes maze (spatial memory). The MCAo + scrambled group showed reduced preference for a novel object after the stroke and poor learning in the spatial memory task. In contrast, mir363-3p treated animals were similar to either their baseline performance or to the sham group. Histological analysis showed significant deterioration of specific white matter tracts due to stroke, which was attenuated in mir363-3p treated animals. The present data builds on our previous finding to show that a neuroprotectant can abrogate the long-term effects of stroke.

show abstract

Section: Methodsmentioning

confidence: 99%

“…This test was performed as described in our previous study (Panta et al, 2019 ). This test consisted of three phases: habituation, familiarization, and test phase.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations