Miraxanthin-V, Liriodenin and Chitranone are Hepcidin Antagonist In silico for Iron Deficiency Anemia

Yotriana, Safirah .; Suselo, Yuliana Heri; Muthmainah, Muthmainah; Indarto, Dono

doi:10.1088/1757-899x/333/1/012079

Cited by 3 publications

(2 citation statements)

References 2 publications

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“…Indicaxanthin play a role as an erythropoietin agonist which will increase the expression of matriptase-2. Miraxanthin V and Boeravinone F can neutralize hepcidin and inhibit hepcidin-ferroportin binding [4][5][6]. In silico, M. jalapa has also been developed for cancer and stroke therapy [7,8].…”

Section: Introductionmentioning

confidence: 99%

Alkaloid Fraction of Mirabilis Jalapa Leaves has Higher Betaxanthin Levels than Ethanol Extract and is Potentially Developed for Anemia Treatment

Suselo¹,

Indarto²,

Wasita³

et al. 2022

Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)

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Mirabilis jalapa is a medicinal plant, which is widely distributed in some tropical countries including in Indonesia. Indicaxanthin, Miraxanthin and Boeravinone F active compounds, which are the family member of Betaxanthins in the M. jalapa plant, are able to inhibit hepcidin and to activate matriptase-2 for increase of iron absorption in silico. Meanwhile, Betalains compound belongs to alkaloid compound, which is the precursor for Betaxanthin and Betacyanin synthesis. Therefore, this study aimed to identify Betaxanthin, Betacyanin and Betalain compounds in extract and fraction of M. jalapa leaves. Dried M. jalapa leaves were purchased from Merapi Farma, Yogyakarta. Extraction and fractionation of M. jalapa leaves were carried out using maceration and physical methods respectively. Alkaloid compounds in ethanol extract and alkaloid fraction were identified using a thin layer chromatography with piperine as the standard compound. Betaxanthin, Betacyanin and Betalain levels were measured using the UV-Vis spectrophotometry. From M. jalapa simplicia, it yielded only 7.7% ethanol extract while we got 41.33% alkaloid fraction. Alkaloid compounds were only detected in M. jalapa leaves fraction instead of the ethanol extract. Betaxanthin levels in alkaloid fraction were 23 times higher than that of the ethanol extract. Higher Betaxanthin levels were also observed in ethanol extract, compared to Betacyanin and Betalain levels. The alkaloid fraction of M. jalapa leaves is a better source of Betaxanthin compound and its derivatives than the ethanol extract. Further investigation is needed to determine phytochemicals compounds in the alkaloid fraction of M. jalapa leaves and their role in iron metabolism.

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Section: Introductionmentioning

confidence: 99%

Alkaloid Fraction of Mirabilis Jalapa Leaves has Higher Betaxanthin Levels than Ethanol Extract and is Potentially Developed for Anemia Treatment

Suselo¹,

Indarto²,

Wasita³

et al. 2022

Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)

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“…In previous study, we found several phytochemicals in silico using molecular docking approach that play a role in iron metabolism. Indicaxanthin, Miraxanthin, and Boeravinone F, the member of Betaxanthin group found in Mirabilis jalapa have potentially developed as antagonist hepcidin and erythropoietin agonist [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

The Cytotoxicity Effect of Ethanol Extract and Alkaloid Fraction of Mirabilis jalapa Leaves in Hepatocarcinoma Cell Line

Suselo¹,

Indarto²,

Wasita³

et al. 2022

Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)

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Mirabilis jalapa is a dry tropical plant which is well grown in Indonesia. Betaxanthin active compound is present in the M. jalapa plant, which plays a role in iron metabolism. Cytotoxicity activity is one important assay for drug development. Therefore, this study aimed to determine whether ethanol extract and alkaloid fraction of M. jalapa leaves had a cytotoxic effect or not in hepatocarcinoma (HepG2) cell line. M. jalapa plants were cultivated in Merapi Farma, Yogyakarta. Extraction and fractionation of M. jalapa leaves were performed in the Pharmacy Laboratory, Surakarta. HepG2 cell line was subcultured in a complete culture media. Confluent cells were then treated with extract and fraction of M. jalapa leaves for 24 h. To determine the toxic dose of extract and fraction of M. jalapa leaves, we used tetrazolium bromide (MTT) and Resazurin assays, which was indicated as 50% inhibitory concentration (IC 50 ). In the MTT assay, IC 50 of ethanol extract was 51.69 ppm, higher than the IC 50 of alkaloid fraction (47.79 ppm). In comparison with the Resazurin assay, the IC 50 both ethanol and alkaloid fraction were higher than that of the MTT assay (135.87 and 149.08 ppm respectively). Furthermore, these toxic doses were still safe for HepG2 cells (>30 ppm). Ethanol extract and alkaloid fraction of M. jalapa leaves have low cytotoxic effect in HepG2 cell line. The next research is required to evaluate the effect of extract and fraction of M. jalapa leaves on iron metabolism in HepG2 cells.

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Alkaloid fraction of Mirabilis jalapa Linn. flowers has low cytotoxicity and increases iron absorption through Erythropoietin-Matriptase-2-Hepcidin pathway in iron deficiency Hepatocarcinoma cell model

Suselo

Indarto

Wasita

et al. 2023

Saudi Journal of Biological Sciences

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Miraxanthin-V, Liriodenin and Chitranone are Hepcidin Antagonist In silico for Iron Deficiency Anemia

Cited by 3 publications

References 2 publications

Alkaloid Fraction of Mirabilis Jalapa Leaves has Higher Betaxanthin Levels than Ethanol Extract and is Potentially Developed for Anemia Treatment

Alkaloid Fraction of Mirabilis Jalapa Leaves has Higher Betaxanthin Levels than Ethanol Extract and is Potentially Developed for Anemia Treatment

The Cytotoxicity Effect of Ethanol Extract and Alkaloid Fraction of Mirabilis jalapa Leaves in Hepatocarcinoma Cell Line

Alkaloid fraction of Mirabilis jalapa Linn. flowers has low cytotoxicity and increases iron absorption through Erythropoietin-Matriptase-2-Hepcidin pathway in iron deficiency Hepatocarcinoma cell model

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